| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,776,358 | G→T | 33.3% | L16L (CTG→CTT) | yfiB → | predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,776,358 | 0 | G | T | 33.3% | 30.3 / 16.6 | 30 | L16L (CTG→CTT) | yfiB | predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (10/10); new base T (5/5); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGATAACAAGGATATATCGATATGATAAAGCACCTGGTAGCACCCCTGGTTTTCACCTCACTAATACTGACTGGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCTTATGGATT > minE/1776290‑1776426 | agaTAACAAGGATATATCGATATGATAAAGCACCTGGTAGCACCCCTGGTTTTCACCTCACTAATactg < 1:2838722/69‑1 (MQ=255)agaTAACAAGGATATATCGATATGATAAAGCACCTGGTAGCACCCCTGGTTTTCACCTCACTAATactg < 1:1738447/69‑1 (MQ=255) atatCGATATGATAAAGCACCTGGTAGCACCCCTGGTTTTCACCTCACTAATACTGACTGGCTGCCAGt < 1:1105060/69‑1 (MQ=255) atatCGATATGATAAAGCACCTGGTAGCACCCCTGGTTTTCACCTCACTAATACTGACTGGCTGCCAGt < 1:2587874/69‑1 (MQ=255) atatCGATATGATAAAGCACCTGGTAGCACCCCTGGTTTTCACCTCACTAATACTGACTGGCTGCCAGt < 1:2782386/69‑1 (MQ=255) tCGATATGATAAAGCACCTGGTAGCACCCCTGGTTTTCACCTCACTAATACTGACTGGCTGCCAGTccc > 1:850751/1‑69 (MQ=255) tCGATATGATAAAGCACCTGGTAGCACCCCTGGTTTTCACCTCACTAATACTGACTGGCTGCCAGTccc > 1:2648371/1‑69 (MQ=255) tCGATATGATAAAGCACCTGGTAGCACCCCTGGTTTTCACCTCACTAATACTGACTGGCTGCCAGTccc > 1:564183/1‑69 (MQ=255) aaGCACCTGGTAGCACCCCTGGTTTTCACCTCACTAATACTGACTGGCTGCCAGTCCCCTCAGGGAAAg < 1:2415376/69‑1 (MQ=255) aaGCACCTGCTAGCACCCCTGATTTTCACCTCGCTAATACTTACTGGCTGCCAGTCCCCTCAGGGAAAg > 1:1345237/1‑69 (MQ=255) aaGCACCTGCTAGCACCCCTGATTTTCACCTCGCTAATACTTACTGGCTGCCAGTCCCCTCAGGGAAAg > 1:727844/1‑69 (MQ=255) aaGCACCTGCTAGCACCCCTGATTTTCACCTCGCTAATACTTACTGGCTGCCAGTCCCCTCAGGGAAAg > 1:1339137/1‑69 (MQ=255) gCACCTGGTAGCACCCCTGGTTTTCACCTCACTAATACTGACTGGCTGCCAGTCCCCTCAGGGAAAGtt < 1:2310587/69‑1 (MQ=255) gCACCTGGTAGCACCCCTGGTTTTCACCTCACTAATACTGACTGGCTGCCAGTCCCCTCAGGGAAAGtt < 1:1797482/69‑1 (MQ=255) gCACCTGGTAGCACCCCTGGTTTTCACCTCACTAATACTGACTGGCTGCCAGTCCCCTCAGGGAAAGtt < 1:1825689/69‑1 (MQ=255) agaaCCCCTGGTTTTCACCTCACTAATACTGACTGGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTg > 1:625271/4‑68 (MQ=255) aGCACCCCTGGTTTTCACCTCACTAATACTGACTGGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTg > 1:2783184/1‑68 (MQ=255) gTTTTCACCTCACTAATACTGACTGGCTGCCAGTCCCCTCAGGGAAAGTTTAGTCCTGAGCAAGTcgcc > 1:3210366/1‑69 (MQ=255) gTTTTCACCTCACTAATACTGACTGGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTcgcc > 1:2754357/1‑69 (MQ=255) gTTTTCACCTCACTAATACTGACTGGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTcgcc > 1:3176491/1‑69 (MQ=255) gTTTTCACCTCACTAATACTGACTGGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTcgc > 1:2811819/1‑68 (MQ=255) tttCACCTCACTAATACTGACTGGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTcgccgc < 1:2594862/69‑1 (MQ=255) tttCACCTCACTAATACTGACTGGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTcgccgc < 1:537631/69‑1 (MQ=255) ttCACCTCACTAATACTGACTGGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCt > 1:172842/1‑69 (MQ=255) tAATACTTACTGGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATc < 1:1065184/67‑1 (MQ=255) tAATACTTACTGGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATc < 1:2961487/67‑1 (MQ=255) tAATACTTACTGGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATc < 1:338385/67‑1 (MQ=255) tAATACTTACTGGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATc < 1:37543/67‑1 (MQ=255) tAATACTTACTGGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATc < 1:1753512/67‑1 (MQ=255) tAATACTTACTGGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCtt > 1:1815851/1‑69 (MQ=255) tAATACTTACTGGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCtt > 1:1445275/1‑69 (MQ=255) tAATACTGACTGGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCt < 1:389276/68‑1 (MQ=255) actgactgGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCTTATgg > 1:2308842/1‑69 (MQ=255) actgactgGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCTTATgg > 1:2181859/1‑69 (MQ=255) actgactgGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCTTATgg > 1:2466723/1‑69 (MQ=255) actgactgGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCTTATgg > 1:1366404/1‑69 (MQ=255) ctgactgGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCTTATGGa > 1:713675/1‑69 (MQ=255) gactgGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCTTATGGAtt > 1:1032050/1‑69 (MQ=255) gactgGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCTTATGGAtt < 1:1180057/69‑1 (MQ=255) gactgGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCTTATGGAtt > 1:67127/1‑69 (MQ=255) gactgGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCTTATGGAtt > 1:1304560/1‑69 (MQ=255) gactgGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCTTATGGAtt < 1:1392763/69‑1 (MQ=255) gactgGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCTTATGGAtt < 1:3037979/69‑1 (MQ=255) gactgGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCTTATGGAtt > 1:1907288/1‑69 (MQ=255) gactgGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCTTATGGAtt < 1:2953903/69‑1 (MQ=255) gactgGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCTTATGGAtt < 1:1914560/69‑1 (MQ=255) gactgGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCTTATGGAtt < 1:2070853/69‑1 (MQ=255) gactgGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCTTATGGAt > 1:1570487/1‑68 (MQ=255) | AGATAACAAGGATATATCGATATGATAAAGCACCTGGTAGCACCCCTGGTTTTCACCTCACTAATACTGACTGGCTGCCAGTCCCCTCAGGGAAAGTTTACTCCTGAGCAAGTCGCCGCTATGCAATCTTATGGATT > minE/1776290‑1776426 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |