| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,779,576 | C→T | 55.7% | S302S (TCG→TCA) | ffh ← | Signal Recognition Particle (SRP) component with 4.5S RNA (ffs) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,779,576 | 0 | C | T | 55.7% | 15.4 / 62.8 | 79 | S302S (TCG→TCA) | ffh | Signal Recognition Particle (SRP) component with 4.5S RNA (ffs) |
| Reads supporting (aligned to +/- strand): ref base C (10/25); new base T (27/17); total (37/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.13e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01 | |||||||||||
AGCTTGCTGGCTAATTTCTCTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGC > minE/1779514‑1779633 | aGCTTGCTGGCTAATTTCTCTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAg < 1:1084503/68‑1 (MQ=255) cTTGCTGGCTAATTTCTCTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTAc > 1:1771532/1‑69 (MQ=255) ggCTAATTTCTCTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGcc < 1:718467/69‑1 (MQ=255) ggCTAATTTCTCTGCCTGCACGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGcc < 1:568842/69‑1 (MQ=255) ggCTAATTTCTCTGCCTGCACGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGcc < 1:2921366/69‑1 (MQ=255) ggCTAATTTCTCTGCCTGCACGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGcc < 1:1186813/69‑1 (MQ=255) ggCTAATTTCTCTGCCTGCACGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGcc < 1:3319283/69‑1 (MQ=255) tctcTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCg > 1:1119392/1‑68 (MQ=255) tctcTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCg > 1:606736/1‑68 (MQ=255) tctcTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCg > 1:1088139/1‑68 (MQ=255) tctcTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCg > 1:2879908/1‑68 (MQ=255) tctcTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCg > 1:1052680/1‑68 (MQ=255) tctcTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCg > 1:1838912/1‑68 (MQ=255) tctcTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCg > 1:1065896/1‑68 (MQ=255) cctgcGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGaa < 1:2620374/66‑1 (MQ=255) cctgcGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGaa < 1:948028/66‑1 (MQ=255) cctgcGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGaa < 1:2100838/66‑1 (MQ=255) tgcGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATacgc < 1:399430/69‑1 (MQ=255) tgcGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATacgc < 1:3260916/69‑1 (MQ=255) gcgcgGTCAACCTTGCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATacggga > 1:212494/1‑68 (MQ=255) gcgcgGTCAACCTTGCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATacggga > 1:275705/1‑68 (MQ=255) gcgcgGTCAACCTTGCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATacggga > 1:2816514/1‑68 (MQ=255) gcgcgGTCAACCTTGCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATacggga > 1:2670090/1‑68 (MQ=255) gcgcgGTCAACCTTGCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATacggga > 1:2902263/1‑68 (MQ=255) gcgcgGTCAACCTTGCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATacggga > 1:2298664/1‑68 (MQ=255) gcgcgGTCAACCTTGCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATacggga > 1:2960011/1‑68 (MQ=255) gcgcgGTCAACCTTGCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATacggga > 1:3063775/1‑68 (MQ=255) gcgcgGTCAACCTTGCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATacggga > 1:2234551/1‑68 (MQ=255) gcgcgGTCAACCTTGCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATacggga > 1:838424/1‑68 (MQ=255) gcgcgGTCAACCTTGCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATacggga > 1:2157221/1‑68 (MQ=255) gcgcgGTCAACCTTGCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATacggga > 1:140780/1‑68 (MQ=255) gcgcgGTCAACCTTGCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATacggga > 1:1898010/1‑68 (MQ=255) gcgcgGTCAACCTTGCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATacggga > 1:1860394/1‑68 (MQ=255) gcgcgGTCAACCTTGCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATacggga > 1:1525873/1‑68 (MQ=255) gcgcgGTCAACCTTGCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATacggga > 1:875172/1‑68 (MQ=255) gcgcgGTCAACCTTGCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATacggga > 1:116071/1‑68 (MQ=255) gcgcgGTCAACCTTGCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATacggga > 1:1106342/1‑68 (MQ=255) gcgcgGTCAACCTTGCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATacggg > 1:755325/1‑65 (MQ=255) gCTTTCGATATCTTCGATCAGTGACAGTACGTCGc > 1:176525/1‑35 (MQ=255) gCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATACGGGAGGCAATACGGTCCgg > 1:697971/1‑69 (MQ=255) gCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATACGGGAGGCAATACGGTCCgg > 1:735357/1‑69 (MQ=255) gCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATACGGGAGGCAATACGGTCCgg > 1:2922752/1‑69 (MQ=255) gCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATACGGGAGGCAATACGGTCCgg > 1:1259791/1‑69 (MQ=255) gCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATACGGGAGGCAATACGGTCCgg > 1:1303950/1‑69 (MQ=255) gCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATACGGGAGGCAATACGGTCCgg > 1:2486217/1‑69 (MQ=255) gCTTTCGATATCTTCGATCAGTGACAGTACGTCGCCCATGCCGAGAATACGGGAGGCAATACGGTCCg > 1:1570805/1‑68 (MQ=255) gATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCTATGCGGTCCGGATgg < 1:1072393/67‑1 (MQ=255) gATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATgg < 1:1179813/67‑1 (MQ=255) gATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATgg < 1:1954055/67‑1 (MQ=255) gATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATgg < 1:205862/67‑1 (MQ=255) gATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATgg < 1:3074017/67‑1 (MQ=255) gATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATgg < 1:31589/67‑1 (MQ=255) gATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATgg < 1:3161034/67‑1 (MQ=255) gATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATgg < 1:3205580/67‑1 (MQ=255) gATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGa < 1:809821/68‑1 (MQ=255) gATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGa < 1:3398208/68‑1 (MQ=255) gATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGa < 1:2291146/68‑1 (MQ=255) gATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGa < 1:1290014/68‑1 (MQ=255) gATATCTTCGATCAGCGACAGTACGGCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATgg < 1:464721/67‑1 (MQ=255) atatCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATgg < 1:173907/66‑1 (MQ=255) ttCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGc > 1:2568135/1‑67 (MQ=255) ttCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGc > 1:2829810/1‑67 (MQ=255) tCGATCAGTGACAGTACGTCGCCCATGCCGAGAATac < 1:1571855/37‑1 (MQ=255) tCGATCAGTGACAGTACGTCGCCCATGCCGAGAATac < 1:963945/37‑1 (MQ=255) tCGATCAGTGACAGTACGTCGCCCATGCCGAGAATac < 1:1089552/37‑1 (MQ=255) tCGATCAGTGACAGTACGTCGCCCATGCCGAGAATac < 1:820480/37‑1 (MQ=255) tCGATCAGTGACAGTACGTCGCCCATGCCGAGAATac < 1:774846/37‑1 (MQ=255) tCGATCAGTGACAGTACGTCGCCCATGCCGAGAATac < 1:666778/37‑1 (MQ=255) tCGATCAGTGACAGTACGTCGCCCATGCCGAGAATac < 1:624387/37‑1 (MQ=255) tCGATCAGTGACAGTACGTCGCCCATGCCGAGAATac < 1:463847/37‑1 (MQ=255) tCGATCAGTGACAGTACGTCGCCCATGCCGAGAATac < 1:328889/37‑1 (MQ=255) tCGATCAGTGACAGTACGTCGCCCATGCCGAGAATac < 1:2828252/37‑1 (MQ=255) tCGATCAGTGACAGTACGTCGCCCATGCCGAGAATac < 1:2678477/37‑1 (MQ=255) tCGATCAGTGACAGTACGTCGCCCATGCCGAGAATac < 1:2258710/37‑1 (MQ=255) tCGATCAGTGACAGTACGTCGCCCATGCCGAGAATac < 1:2198576/37‑1 (MQ=255) tCGATCAGTGACAGTACGTCGCCCATGCCGAGAATac < 1:1715908/37‑1 (MQ=255) tCGATCAGTGACAGTACGTCGCCCATGCCGAGAATac < 1:1638458/37‑1 (MQ=255) tCGATCAGTGACAGTACGTCGCCCATGCCGAGAATac < 1:1597262/37‑1 (MQ=255) gATCAGTGACAGTACGTCGCCCATGCCGAGAATac < 1:3236278/35‑1 (MQ=255) | AGCTTGCTGGCTAATTTCTCTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGC > minE/1779514‑1779633 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |