| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,780,767 | T→C | 41.3% | I65I (ATT→ATC) | ypjD → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,780,767 | 0 | T | C | 41.3% | 12.1 / 31.6 | 46 | I65I (ATT→ATC) | ypjD | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (13/14); new base C (15/4); total (28/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.44e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTGGCGGCGCATGGCTATTATTTCTGCGGTCATTGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAAC > minE/1780704‑1780833 | tGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTGGCGGCGCATGGCTATTATTTCTGCGGTCATCgcgc < 1:567339/68‑1 (MQ=255)tGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTGGCGGCGCATGGCTATTATTTCTGCGGTCATCgcgc < 1:2190790/68‑1 (MQ=255)tGTTCCCGGGCTGCTGCAAAAAAACGGCGGCTGGCGGCGCATGGCTATTATTTCTGCGGTCATCgcgc < 1:496903/68‑1 (MQ=255) gcAAAAAAACGGCGGCTGGCGGCGCATGGCTATTATTTCTGCGGTCATTGCGCTGGTCTGCCACGCa < 1:2145208/67‑1 (MQ=255) gcAAAAAAACGGCGGCTGGCGGCGCATGGCTATTATTTCTGCGGTCATTGCGCTGGTCTGCCACGCa < 1:2052175/67‑1 (MQ=255) gcAAAAAAACGGCGGCTGGCGGCGCATGGCTATTATTTCTGCGGTCATTGCGCTGGTCTGCCACGCa < 1:1438243/67‑1 (MQ=255) aaaaaaCGGCGGCTGGCGGCGCATGGCTATTATTTCTGCGGTCATTGCGCTGGTCTGCCACGCAATCGc < 1:3015904/69‑1 (MQ=255) aaaaaaCGGCGGCTGGCGGCGCATGGCTATTATTTCTGCGGTCATTGCGCTGGTCTGCCACGCAATCGc < 1:2145767/69‑1 (MQ=255) aaaaaaCGGCGGCTGGCGGCGCATGGCTATTATTTCTGCGGTCATTGCGCTGGTCTGCCACGCAATCGc < 1:1825790/69‑1 (MQ=255) aaaaaaCGGCGGCTGGCGGCGCATGGCTATTATTTCTGCGGTCATTGCGCTGGTCTGCCACGCAATCGc < 1:713844/69‑1 (MQ=255) aaaaaCGGCGGCTGGCGGCGCATGGCTATTATTTCTGCGGTCATTGCGCTGGTCTGCCACGCAATCGc < 1:2195848/68‑1 (MQ=255) cggcggCTGGCGGCGCATGGCTATTATTTCTGCGGTCATTGCGCTGGTCTGCCACGCAATCGc < 1:743170/63‑1 (MQ=255) cggcggCTGGCGGCGCATGGCTATTATTTCTGCGGTCATCgcgc < 1:2367841/44‑1 (MQ=255) gCTATTATTTCTGCGGTCATTGCGCTGGTCTGCCCCGCAATCGCTCTGGAAGCCCGCATCCTGCCCGAc > 1:2731316/1‑69 (MQ=255) gCTATTATTTCTGCGGTCATTGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGa > 1:3360703/1‑68 (MQ=255) gCTATTATTTCTGCGGTCATTGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGAc > 1:2160081/1‑69 (MQ=255) attattTCTGCGGTCATTGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGt < 1:2801466/69‑1 (MQ=255) attattTCTGCGGTCATTGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGt < 1:202706/69‑1 (MQ=255) attattTCTGCGGTCATTGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGt < 1:1928514/69‑1 (MQ=255) attTCTGCGGTCATTGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGa > 1:1578553/1‑68 (MQ=255) attTCTGCGGTCATTGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGAt > 1:851681/1‑69 (MQ=255) attTCTGCGGTCATTGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGAt > 1:1355433/1‑69 (MQ=255) attTCTGCGGTCATTGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGAt > 1:1191100/1‑69 (MQ=255) attTCTGCGGTCATTGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGAt > 1:2638163/1‑69 (MQ=255) tCTGCGGTCATTGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAg > 1:630616/1‑68 (MQ=255) gTCATTGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAg > 1:2148472/1‑62 (MQ=255) gTCATTGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACaa > 1:797961/1‑69 (MQ=255) gTCATTGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACaa > 1:2608765/1‑69 (MQ=255) tCATTGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACaaa < 1:1294418/69‑1 (MQ=255) tCATTGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACaa < 1:1547548/68‑1 (MQ=255) tCATCGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCGGCATCCTGCCCGACGGTGATAGCGGACaaa > 1:85066/1‑69 (MQ=255) tCATCGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACaaa > 1:996315/1‑69 (MQ=255) tCATCGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACaaa > 1:2983295/1‑69 (MQ=255) tCATCGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACaaa > 1:992136/1‑69 (MQ=255) tCATCGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACaaa > 1:864341/1‑69 (MQ=255) tCATCGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACaaa > 1:2407096/1‑69 (MQ=255) tCATCGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACaaa > 1:1291655/1‑69 (MQ=255) tCATCGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACaaa > 1:2363003/1‑69 (MQ=255) tCATCGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACaaa > 1:2283536/1‑69 (MQ=255) tCATCGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACaaa > 1:2185059/1‑69 (MQ=255) tCATCGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACaaa > 1:2182705/1‑69 (MQ=255) tCATCGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACaaa > 1:2140617/1‑69 (MQ=255) tCATCGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACaaa > 1:2055594/1‑69 (MQ=255) tCATCGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACaaa > 1:1879576/1‑69 (MQ=255) tCATCGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACaaa > 1:1419126/1‑69 (MQ=255) cATTGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACaaaa > 1:991370/1‑69 (MQ=255) tGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCt > 1:386946/1‑42 (MQ=255) tGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAAc > 1:1238808/1‑67 (MQ=255) tGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAAc > 1:250873/1‑67 (MQ=255) tGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAAc > 1:940014/1‑67 (MQ=255) tGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAAc > 1:875934/1‑67 (MQ=255) tGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAAc > 1:1534847/1‑67 (MQ=255) tGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAAc > 1:3374997/1‑67 (MQ=255) tGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAAc > 1:2452378/1‑67 (MQ=255) | TGTTCCCGGTCTGCTGCAAAAAAACGGCGGCTGGCGGCGCATGGCTATTATTTCTGCGGTCATTGCGCTGGTCTGCCACGCAATCGCTCTGGAAGCCCGCATCCTGCCCGACGGTGATAGCGGACAAAAC > minE/1780704‑1780833 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |