| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 133,667 | G→A | 22.8% | H225H (CAC→CAT) | panB ← | 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 133,667 | 0 | G | A | 22.8% | 99.0 / 21.2 | 74 | H225H (CAC→CAT) | panB | 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (35/22); new base A (11/6); total (46/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01 | |||||||||||
ATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCC > minE/133601‑133735 | ataCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAAtgtg < 1:172023/69‑1 (MQ=255)ataCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAAtgtg < 1:2387989/69‑1 (MQ=255)ataCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAAtgtg < 1:3376012/69‑1 (MQ=255)ataCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAAtgtg < 1:1072764/69‑1 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGGGAATTTAGGAATGTGa > 1:2310827/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAAtgtgc > 1:2038735/1‑68 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAAtgtg > 1:3424369/1‑68 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAAtgt < 1:2799401/67‑1 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAAtgt < 1:2572229/67‑1 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:228127/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2279654/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2087705/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2402522/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2420758/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2454259/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2698521/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2478893/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2569973/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:265410/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1016487/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2710593/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2885652/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2899960/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:294779/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:3019070/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:421463/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:587515/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:670158/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:685255/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:77487/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1046338/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1486506/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1438063/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1415267/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1755591/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1405073/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1372713/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1138996/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1936770/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2010848/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1122291/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2052771/1‑69 (MQ=255) taCTGCCGCACAGCCACGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:377213/1‑69 (MQ=255) cacaGCCGCGCGTATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:831421/69‑1 (MQ=255) cacaGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:1025143/69‑1 (MQ=255) cacaGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:2838098/69‑1 (MQ=255) cacaGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:1083309/69‑1 (MQ=255) cacaGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:1459753/69‑1 (MQ=255) cacaGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:159999/69‑1 (MQ=255) cacaGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:2625244/69‑1 (MQ=255) cacaGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:1836560/69‑1 (MQ=255) acaGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTa < 1:2558379/69‑1 (MQ=255) gcgGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCaaa < 1:626881/69‑1 (MQ=255) gcgGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCaaa < 1:191674/69‑1 (MQ=255) cgGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCaaa < 1:2073569/68‑1 (MQ=255) gATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCaaa < 1:1396016/66‑1 (MQ=255) aTGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAAtgtg < 1:2880742/47‑1 (MQ=255) tCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:327985/53‑1 (MQ=255) tCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:600506/53‑1 (MQ=255) tCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:2032742/53‑1 (MQ=255) tCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:1680878/53‑1 (MQ=255) tCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:381288/53‑1 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGATTTTAGGAATATGACCGCCGGTAATGCCGAACGCGTCGTGc > 1:3106266/1‑68 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGAATTTAGGAATATGACCGCCGGTAAt > 1:2822524/1‑52 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGAATTTAGGAATATGACCGCCGGTAATGCCGAACGCGTCGTGc > 1:37291/1‑68 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGAATTTAGGAATATGACCGCCGGTAATGCCGAACGCGTCGTGc > 1:2251736/1‑68 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGAATTTAGGAATATGACCGCCGGTAATGCCGAACGCGTCGTGc > 1:2313557/1‑68 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGAATTTAGGAATATGACCGCCGGTAATGCCGAACGCGTCGTGc > 1:3235588/1‑68 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGAATTTAGGAATATGACCGCCGGTAATGCCGAACGCGTCGTGc > 1:3185346/1‑68 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGAATTTAGGAATATGACCGCCGGTAATGCCGAACGCGTCGTGc > 1:1005951/1‑68 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGAATTTAGGAATATGACCGCCGGTAATGCCGAACGCGTCGTGc > 1:1179660/1‑68 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGAATTTAGGAATATGACCGCCGGTAATGCCGAACGCGTCGTGc > 1:1905814/1‑68 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGAATTTAGGAATATGACCGCCGGTAATGCCGAACGCGTCGTGc > 1:2628821/1‑68 (MQ=255) gCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:3249705/41‑1 (MQ=255) aaTTTTTGGCGAATTTAGGAATATGACCGCCGGTAAtg < 1:1061491/38‑2 (MQ=38) aaTTTTTGGCGAATTTAGGAATATGACCGCCGGTAAtg < 1:905682/38‑2 (MQ=38) aaTTTTTGGCGAATTTAGGAATATGACCGCCGGTAAtg < 1:368518/38‑2 (MQ=38) aaTTTTTGGCGAATTTAGGAATATGACCGCCGGTAAtg < 1:110853/38‑2 (MQ=38) aaTTTTTGGCGAATTTAGGAATATGACCGCCGGTAAtg < 1:2970030/38‑2 (MQ=38) aaTTTTTGGCGAATTTAGGAATATGACCGCCGGTAAtg < 1:1943256/38‑2 (MQ=38) ttAGGAATGTGAGCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTg < 1:78467/69‑1 (MQ=255) ttAGGAATGTGACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTg < 1:2320297/69‑1 (MQ=255) ttAGGAATGTGACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTg < 1:2053693/69‑1 (MQ=255) gtgACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCgcc < 1:404126/69‑1 (MQ=255) gtgACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCgcc < 1:3394497/69‑1 (MQ=255) gtgACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCgcc < 1:3055031/69‑1 (MQ=255) gtgACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCgcc < 1:2762769/69‑1 (MQ=255) gtgACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCgcc < 1:840129/69‑1 (MQ=255) | ATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCAAAGGCGTCGTGCATCACGAGGATCTGCCCGTCAGTGACGTTGCCTGCGCC > minE/133601‑133735 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |