| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,781,690 | C→T | 18.6% | A47A (GCC→GCT) | yfjD → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,781,690 | 0 | C | T | 18.6% | 124.3 / 17.1 | 80 | A47A (GCC→GCT) | yfjD | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (26/39); new base T (7/8); total (33/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGA > minE/1781636‑1781751 | aGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCAc < 1:3330570/69‑1 (MQ=255)aGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCAc < 1:1926195/69‑1 (MQ=255)aGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCAc < 1:1233766/69‑1 (MQ=255)aGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCAc < 1:73585/69‑1 (MQ=255)aGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCAc < 1:2515442/69‑1 (MQ=255)aGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCAc < 1:2527170/69‑1 (MQ=255)aGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCAc < 1:1447130/69‑1 (MQ=255) gACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCAc < 1:3415152/68‑1 (MQ=255) gACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCAc < 1:1915364/68‑1 (MQ=255) gACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCAc < 1:62715/68‑1 (MQ=255) gACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCAc < 1:790044/68‑1 (MQ=255) gACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACt < 1:1425186/69‑1 (MQ=255) gACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACt < 1:141617/69‑1 (MQ=255) gACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACt < 1:1568876/69‑1 (MQ=255) gACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACt < 1:720225/69‑1 (MQ=255) gACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACt < 1:1150781/69‑1 (MQ=255) cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGTCACTAttgtt < 1:2981319/69‑1 (MQ=255) cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTAttgtt < 1:142868/69‑1 (MQ=255) cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTAttgtt < 1:2577819/69‑1 (MQ=255) cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTAttgtt < 1:3250214/69‑1 (MQ=255) cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTAttgtt < 1:795239/69‑1 (MQ=255) cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTAttgtt < 1:1908303/69‑1 (MQ=255) cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTAttgtt < 1:2844175/69‑1 (MQ=255) cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTAttgtt < 1:1426249/69‑1 (MQ=255) tAAGCCTGGTGTTAATCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGc > 1:2427475/1‑56 (MQ=37) tAAGCCTGGTGTTAATCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCggg > 1:2574209/1‑68 (MQ=255) tAAGCCTGGTGTTAATCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGa > 1:1077078/1‑69 (MQ=255) tAAGCCTGGTGTTAATCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGa > 1:358299/1‑69 (MQ=255) tAAGCCTGGTGTTAATCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGa > 1:2081009/1‑69 (MQ=255) tAAGCCTGGTGTTAATCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGa > 1:562729/1‑69 (MQ=255) tAAGCCTGGTGTTAATCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGa > 1:1910621/1‑69 (MQ=255) aaGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGAt > 1:206445/1‑69 (MQ=255) aaGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGAt > 1:1487992/1‑69 (MQ=255) aaGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGAt > 1:2650515/1‑69 (MQ=255) ttAATCGGCAATAACCTGGTCAATATTCTTGCCTc > 1:615845/1‑35 (MQ=255) ttAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTAc > 1:2387500/1‑69 (MQ=255) ttAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTAc > 1:1378778/1‑69 (MQ=255) tCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc < 1:3033260/68‑1 (MQ=255) tCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc < 1:635200/68‑1 (MQ=255) tCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc < 1:675052/68‑1 (MQ=255) tCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc < 1:964196/68‑1 (MQ=255) tCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc < 1:2835426/68‑1 (MQ=255) tCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc < 1:194329/68‑1 (MQ=255) tCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc < 1:1895089/68‑1 (MQ=255) cGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc < 1:3029205/67‑1 (MQ=255) ggCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGAt > 1:2041821/1‑69 (MQ=255) aaCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGc > 1:1583131/1‑69 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg > 1:2692847/1‑67 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg < 1:552721/67‑1 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg > 1:505960/1‑67 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg < 1:419064/67‑1 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg < 1:2233189/67‑1 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg < 1:187804/67‑1 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg > 1:12834/1‑67 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg < 1:3083514/67‑1 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg < 1:2505300/67‑1 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg > 1:2963043/1‑67 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg < 1:2883012/67‑1 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg < 1:2846702/67‑1 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg < 1:1066511/67‑1 (MQ=255) ggTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGc < 1:2381755/69‑1 (MQ=255) ggTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGc < 1:2593498/69‑1 (MQ=255) cAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAt < 1:2935050/69‑1 (MQ=255) cAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAt < 1:763862/69‑1 (MQ=255) cAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAt < 1:1239142/69‑1 (MQ=255) atatTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAt < 1:2522312/67‑1 (MQ=255) tatTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAtt > 1:2963841/1‑67 (MQ=255) tatTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTg > 1:2494517/1‑68 (MQ=255) tatTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATCGc > 1:539002/1‑69 (MQ=255) atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCg > 1:2816003/1‑58 (MQ=255) atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGc > 1:2087856/1‑68 (MQ=255) atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGc > 1:2399167/1‑68 (MQ=255) atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGc > 1:2820023/1‑68 (MQ=255) atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCg > 1:477606/1‑69 (MQ=255) atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCg > 1:3341696/1‑69 (MQ=255) atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCg > 1:187557/1‑69 (MQ=255) atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCg > 1:2841937/1‑69 (MQ=255) atTCTTGCCTCCGCGCTCGGCACTATTGGTGGGATGCGttt > 1:2824633/1‑41 (MQ=255) ttCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGa > 1:3246407/1‑69 (MQ=255) ttCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGa > 1:1280030/1‑69 (MQ=255) | AGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGA > minE/1781636‑1781751 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |