Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,781,711 T→C 15.1% V54V (GTT→GTC yfjD → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,781,7110TC15.1% 157.0 / 14.5 92V54V (GTT→GTCyfjDpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (52/26);  new base C (6/8);  total (58/34)
Fisher's exact test for biased strand distribution p-value = 1.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01

Only 100 of 101 total aligned reads displayed.

CTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTAC  >  minE/1781643‑1781775
                                                                    |                                                                
cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGTCACTAttgtt                                                                  <  1:2981319/69‑1 (MQ=255)
cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTAttgtt                                                                  <  1:142868/69‑1 (MQ=255)
cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTAttgtt                                                                  <  1:1908303/69‑1 (MQ=255)
cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTAttgtt                                                                  <  1:2844175/69‑1 (MQ=255)
cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTAttgtt                                                                  <  1:3250214/69‑1 (MQ=255)
cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTAttgtt                                                                  <  1:1426249/69‑1 (MQ=255)
cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTAttgtt                                                                  <  1:2577819/69‑1 (MQ=255)
cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTAttgtt                                                                  <  1:795239/69‑1 (MQ=255)
    tAAGCCTGGTGTTAATCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCggg                                                               >  1:2574209/1‑68 (MQ=255)
    tAAGCCTGGTGTTAATCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGa                                                              >  1:562729/1‑69 (MQ=255)
    tAAGCCTGGTGTTAATCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGa                                                              >  1:1910621/1‑69 (MQ=255)
    tAAGCCTGGTGTTAATCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGa                                                              >  1:1077078/1‑69 (MQ=255)
    tAAGCCTGGTGTTAATCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGa                                                              >  1:358299/1‑69 (MQ=255)
    tAAGCCTGGTGTTAATCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGa                                                              >  1:2081009/1‑69 (MQ=255)
     aaGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGAt                                                             >  1:2650515/1‑69 (MQ=255)
     aaGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGAt                                                             >  1:1487992/1‑69 (MQ=255)
     aaGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGAt                                                             >  1:206445/1‑69 (MQ=255)
               ttAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTAc                                                   >  1:2387500/1‑69 (MQ=255)
               ttAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTAc                                                   >  1:1378778/1‑69 (MQ=255)
                   tCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc                                                <  1:3033260/68‑1 (MQ=255)
                   tCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc                                                <  1:675052/68‑1 (MQ=255)
                   tCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc                                                <  1:194329/68‑1 (MQ=255)
                   tCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc                                                <  1:1895089/68‑1 (MQ=255)
                   tCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc                                                <  1:2835426/68‑1 (MQ=255)
                   tCGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc                                                <  1:635200/68‑1 (MQ=255)
                    cGGCAATAATCTGGTCAATATCCTTGCTTCCGCACTCGGCACTATTGTCGGGATGCGCTTGTATGGc                                                <  1:3029205/67‑1 (MQ=255)
                     ggCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGAt                                             >  1:2041821/1‑69 (MQ=255)
                           aaCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGc                                       >  1:1583131/1‑69 (MQ=255)
                            aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                        >  1:2963043/1‑67 (MQ=255)
                            aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                        <  1:2883012/67‑1 (MQ=255)
                            aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                        <  1:2846702/67‑1 (MQ=255)
                            aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                        <  1:187804/67‑1 (MQ=255)
                            aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                        <  1:3083514/67‑1 (MQ=255)
                            aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                        <  1:419064/67‑1 (MQ=255)
                            aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                        >  1:2692847/1‑67 (MQ=255)
                            aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                        >  1:505960/1‑67 (MQ=255)
                            aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                        <  1:2233189/67‑1 (MQ=255)
                            aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                        <  1:552721/67‑1 (MQ=255)
                            aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                        >  1:12834/1‑67 (MQ=255)
                            aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                        <  1:2505300/67‑1 (MQ=255)
                            aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg                                        <  1:1066511/67‑1 (MQ=255)
                                ggTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGc                                  <  1:2593498/69‑1 (MQ=255)
                                ggTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGc                                  <  1:2381755/69‑1 (MQ=255)
                                   cAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAt                               <  1:2935050/69‑1 (MQ=255)
                                   cAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAt                               <  1:763862/69‑1 (MQ=255)
                                   cAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAt                               <  1:1239142/69‑1 (MQ=255)
                                     atatTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAt                               <  1:2522312/67‑1 (MQ=255)
                                      tatTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAtt                              >  1:2963841/1‑67 (MQ=255)
                                      tatTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTg                             >  1:2494517/1‑68 (MQ=255)
                                      tatTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATCGc                            >  1:539002/1‑69 (MQ=255)
                                       atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCg                                      >  1:2816003/1‑58 (MQ=255)
                                       atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGc                            >  1:2399167/1‑68 (MQ=255)
                                       atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGc                            >  1:2820023/1‑68 (MQ=255)
                                       atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGc                            >  1:2087856/1‑68 (MQ=255)
                                       atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCg                           >  1:187557/1‑69 (MQ=255)
                                       atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCg                           >  1:477606/1‑69 (MQ=255)
                                       atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCg                           >  1:2841937/1‑69 (MQ=255)
                                       atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCg                           >  1:3341696/1‑69 (MQ=255)
                                       atTCTTGCCTCCGCGCTCGGCACTATTGGTGGGATGCGttt                                                       >  1:2824633/1‑41 (MQ=255)
                                        ttCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGa                          >  1:1280030/1‑69 (MQ=255)
                                        ttCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGa                          >  1:3246407/1‑69 (MQ=255)
                                                    cgcTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCt                <  1:1527102/67‑1 (MQ=255)
                                                    cgcTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCt                <  1:2904288/67‑1 (MQ=255)
                                                    cgcTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCt                <  1:556798/67‑1 (MQ=255)
                                                    cgcTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCt                <  1:39859/67‑1 (MQ=255)
                                                    cgcTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCt                <  1:568684/67‑1 (MQ=255)
                                                        cGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACttt          >  1:2964842/1‑69 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGc                            >  1:1420443/1‑44 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCg                           >  1:1897545/1‑45 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACt                        >  1:1402707/1‑48 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGTCTTTTGTCGt    >  1:3410559/1‑68 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTc      >  1:3086236/1‑66 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCg     >  1:2747033/1‑67 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt    >  1:239761/1‑68 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt    >  1:730417/1‑68 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt    >  1:2374221/1‑68 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt    >  1:2371700/1‑68 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt    >  1:773059/1‑68 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt    >  1:2098523/1‑68 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt    >  1:2786024/1‑68 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt    >  1:2055274/1‑68 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt    >  1:1560504/1‑68 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt    >  1:1976946/1‑68 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt    >  1:3237664/1‑68 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt    >  1:653992/1‑68 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa   >  1:1347952/1‑69 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa   >  1:1386945/1‑69 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa   >  1:887564/1‑69 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa   <  1:1417593/69‑1 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa   <  1:493486/69‑1 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa   <  1:489925/69‑1 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa   >  1:42187/1‑69 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa   >  1:3359722/1‑69 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa   >  1:1883298/1‑69 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa   <  1:1895713/69‑1 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa   >  1:1921077/1‑69 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa   >  1:2134023/1‑69 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa   <  1:1051496/69‑1 (MQ=255)
                                                               aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa   <  1:2319367/69‑1 (MQ=255)
                                                                attttgGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAATGCGACTGGTGTGCTGACTTTTGTCGTAc  >  1:1753910/4‑69 (MQ=255)
                                                                    |                                                                
CTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTAC  >  minE/1781643‑1781775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: