| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,781,735 | G→T | 37.6% | A62A (GCG→GCT) | yfjD → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,781,735 | 0 | G | T | 37.6% | 104.9 / 91.9 | 117 | A62A (GCG→GCT) | yfjD | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (47/26); new base T (18/26); total (65/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.07e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
Only 100 of 130 total aligned reads displayed.
AACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTAT > minE/1781670‑1781793 | aaCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGc > 1:1583131/1‑69 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg > 1:505960/1‑67 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg < 1:2505300/67‑1 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg < 1:552721/67‑1 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg > 1:2692847/1‑67 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg < 1:419064/67‑1 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg < 1:3083514/67‑1 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg < 1:2883012/67‑1 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg > 1:12834/1‑67 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg < 1:2233189/67‑1 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg < 1:1066511/67‑1 (MQ=255) aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCggg > 1:2963043/1‑67 (MQ=255) ggTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGc < 1:2593498/69‑1 (MQ=255) ggTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGc < 1:2381755/69‑1 (MQ=255) cAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAt < 1:1239142/69‑1 (MQ=255) cAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAt < 1:763862/69‑1 (MQ=255) tatTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAtt > 1:2963841/1‑67 (MQ=255) tatTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTg > 1:2494517/1‑68 (MQ=255) tatTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATCGc > 1:539002/1‑69 (MQ=255) atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCg > 1:2816003/1‑58 (MQ=255) atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGc > 1:2820023/1‑68 (MQ=255) atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGc > 1:2087856/1‑68 (MQ=255) atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGc > 1:2399167/1‑68 (MQ=255) atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCg > 1:3341696/1‑69 (MQ=255) atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCg > 1:187557/1‑69 (MQ=255) atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCg > 1:477606/1‑69 (MQ=255) atTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCg > 1:2841937/1‑69 (MQ=255) ttCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGa > 1:1280030/1‑69 (MQ=255) ttCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGa > 1:3246407/1‑69 (MQ=255) cgcTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCt < 1:556798/67‑1 (MQ=255) cgcTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCt < 1:39859/67‑1 (MQ=255) cgcTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCt < 1:2904288/67‑1 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGc > 1:1420443/1‑44 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCg > 1:1897545/1‑45 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACt > 1:1402707/1‑48 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGTCTTTTGTCGt > 1:3410559/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTc > 1:3086236/1‑66 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:1560504/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:773059/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:730417/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:2786024/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:653992/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:1976946/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:2374221/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:2371700/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:2098523/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:3237664/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa < 1:1051496/69‑1 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:887564/1‑69 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:3359722/1‑69 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:1921077/1‑69 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:1347952/1‑69 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:1386945/1‑69 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa < 1:1895713/69‑1 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:1883298/1‑69 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa < 1:493486/69‑1 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa < 1:489925/69‑1 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:42187/1‑69 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa < 1:2319367/69‑1 (MQ=255) aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct > 1:598451/1‑50 (MQ=37) aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct > 1:952090/1‑50 (MQ=37) aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct > 1:3061494/1‑50 (MQ=37) aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct > 1:3139369/1‑50 (MQ=37) aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct > 1:708668/1‑50 (MQ=37) aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct > 1:1575575/1‑50 (MQ=37) aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct > 1:1469274/1‑50 (MQ=37) aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct > 1:2010940/1‑50 (MQ=37) aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct > 1:1350990/1‑50 (MQ=37) aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct > 1:1323499/1‑50 (MQ=37) aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct > 1:117257/1‑50 (MQ=37) aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct > 1:170944/1‑50 (MQ=37) aTGCGCTTGTATGGCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGAcct > 1:1644877/1‑50 (MQ=37) tttGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGa > 1:2217939/1‑69 (MQ=255) tttGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGa > 1:2201517/1‑69 (MQ=255) tttGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGa > 1:1592228/1‑69 (MQ=255) tttGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGa > 1:185610/1‑69 (MQ=255) tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg < 1:3331871/69‑1 (MQ=255) tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg < 1:2016217/69‑1 (MQ=255) tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg < 1:1740080/69‑1 (MQ=255) tGTACGGCGATGCGGGCGTGGCAATTGAGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg < 1:2555262/69‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1543873/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:705083/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1462760/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1002544/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1171893/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1051553/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:926919/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2940175/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1548315/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1727498/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1816740/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:3373867/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2077059/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:3303441/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2729237/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2759978/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2796098/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2912985/66‑1 (MQ=255) cGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTAt < 1:3166493/68‑1 (MQ=255) cGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTAt < 1:2908020/68‑1 (MQ=255) | AACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTAT > minE/1781670‑1781793 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |