| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,781,765 | T→C | 34.4% | T72T (ACT→ACC) | yfjD → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,781,765 | 0 | T | C | 34.4% | 61.1 / 49.2 | 87 | T72T (ACT→ACC) | yfjD | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (36/21); new base C (4/26); total (40/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCG > minE/1781699‑1781832 | cGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACttt > 1:2964842/1‑69 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGTCTTTTGTCGt > 1:3410559/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTc > 1:3086236/1‑66 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCg > 1:2747033/1‑67 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:3237664/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:2098523/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:2371700/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:2374221/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:239761/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:1560504/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:2786024/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:2055274/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:1976946/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:653992/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:730417/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt > 1:773059/1‑68 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:1883298/1‑69 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:1921077/1‑69 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa < 1:1895713/69‑1 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa < 1:1051496/69‑1 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:2134023/1‑69 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa < 1:2319367/69‑1 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:3359722/1‑69 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:42187/1‑69 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa < 1:489925/69‑1 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa < 1:493486/69‑1 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:887564/1‑69 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:1386945/1‑69 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa < 1:1417593/69‑1 (MQ=255) aTTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa > 1:1347952/1‑69 (MQ=255) attttgGGATGCGTTTGTACGGCGATGCGGGCGTGGCAAATGCGACTGGTGTGCTGACTTTTGTCGTAc > 1:1753910/4‑69 (MQ=255) tttGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGa > 1:185610/1‑69 (MQ=255) tttGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGa > 1:3132694/1‑69 (MQ=255) tttGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGa > 1:1592228/1‑69 (MQ=255) tttGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGa > 1:2217939/1‑69 (MQ=255) tttGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGa > 1:2201517/1‑69 (MQ=255) tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg < 1:382211/69‑1 (MQ=255) tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg < 1:3331871/69‑1 (MQ=255) tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg < 1:1259206/69‑1 (MQ=255) tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg < 1:535957/69‑1 (MQ=255) tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg < 1:2016217/69‑1 (MQ=255) tGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg < 1:1740080/69‑1 (MQ=255) tGTACGGCGATGCGGGCGTGGCAATTGAGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg < 1:2555262/69‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1171893/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1816740/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:48408/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:3373867/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:980345/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:926919/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:3303441/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:3272812/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1462760/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2077059/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1002544/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1543873/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2940175/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2912985/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2796797/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2796098/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1548315/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2759978/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1051553/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1569957/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2729237/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:705083/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:1727498/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:708917/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:170288/66‑1 (MQ=255) atggCGATGCTGGCGTGGCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTa < 1:2324799/66‑1 (MQ=255) cGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTAt < 1:2908020/68‑1 (MQ=255) cGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTAt < 1:3166493/68‑1 (MQ=255) ggCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAAc < 1:458224/68‑1 (MQ=255) ggCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAAc < 1:1895410/68‑1 (MQ=255) ggCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAAc < 1:2189055/68‑1 (MQ=255) ggCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTAt < 1:70601/53‑1 (MQ=255) ggCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTATTGCCGAAAACCATTGc > 1:140396/1‑69 (MQ=255) ggCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTATTGCCGAAAACCATTGc > 1:1980711/1‑69 (MQ=255) ggCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTATTGCCGAAAACCATTGc > 1:1728477/1‑69 (MQ=255) ggCAATTGCGACTGGCGTGCTGACCTTCGTAGTGCTGGTTTTTGCTGAAGTATTGCCGAAAACCATTGc > 1:3147088/1‑69 (MQ=255) gCGACTGGTGTGCTGACTTTTGTCTTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTg > 1:1721356/1‑69 (MQ=255) gCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTg > 1:1620782/1‑69 (MQ=255) tGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAAc < 1:3401566/51‑1 (MQ=255) tgCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAAc < 1:790615/46‑1 (MQ=255) cTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTa > 1:177341/1‑59 (MQ=255) cTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGaaaaa > 1:1465614/1‑69 (MQ=255) cTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGaaaaa > 1:2975779/1‑69 (MQ=255) cTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGaaaaa > 1:3386557/1‑69 (MQ=255) cTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGaaaaa > 1:918640/1‑69 (MQ=255) ttttGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCg < 1:1197884/68‑1 (MQ=255) ttttGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCg < 1:2703448/68‑1 (MQ=255) ttttGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCg < 1:370971/68‑1 (MQ=255) | CGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTACCCGGAAAAAGTCG > minE/1781699‑1781832 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |