Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,782,942	C→T	53.3%	P151P (CCG→CCA)	grpE ←	heat shock protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,782,942	0	C	T	53.3%	5.7 / 64.4	75	P151P (CCG→CCA)	grpE	heat shock protein
Reads supporting (aligned to +/- strand):  ref base C (8/27);  new base T (30/10);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 8.15e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01

ATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTCAACGCCAAACTTACGCACA  >  minE/1782877‑1782996                                                                  |                                                       aTGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTCggg                                                     >  1:1022207/1‑69 (MQ=255) aTGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTCggg                                                     >  1:2644753/1‑69 (MQ=255) aTGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTCggg                                                     >  1:765694/1‑69 (MQ=255) aTGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTCggg                                                     >  1:1230978/1‑69 (MQ=255) aTGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTCggg                                                     >  1:64692/1‑69 (MQ=255) aTGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTCggg                                                     >  1:1372250/1‑69 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGGGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:2375160/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:3265341/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:1054085/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:3258812/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:1929010/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:3155768/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:2868948/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:2091577/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:275178/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:2219817/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:2361611/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:2395654/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:2427140/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:2640290/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:2619587/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:1655368/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:405505/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:577343/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:1611472/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:953436/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:589401/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:1511043/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:627102/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:1281420/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:919609/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:693927/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:1188669/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:793293/1‑68 (MQ=255)        gTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:803154/1‑68 (MQ=255)        ctACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTTGGGTCCAGt                                               >  1:1241082/2‑68 (MQ=255)           cGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGa                                           >  1:2594317/1‑69 (MQ=255)           cGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGa                                           >  1:1874042/1‑69 (MQ=255)                                caGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGAt                           <  1:272677/64‑1 (MQ=255)                                caGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGAt                           <  1:3089770/64‑1 (MQ=255)                                caGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGAt                           <  1:2606477/64‑1 (MQ=255)                                 aGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCTATCACTTc                     <  1:3030663/69‑1 (MQ=255)                                 aGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTc                     <  1:2899788/69‑1 (MQ=255)                                 aGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTc                     <  1:194555/69‑1 (MQ=255)                                 aGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTc                     <  1:1808069/69‑1 (MQ=255)                                 aGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTc                     <  1:1966487/69‑1 (MQ=255)                                 aGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTc                     <  1:2938373/69‑1 (MQ=255)                                 aGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTc                     <  1:2903915/69‑1 (MQ=255)                                 aGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTc                     <  1:466816/69‑1 (MQ=255)                                 aGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTc                     <  1:1358589/69‑1 (MQ=255)                                 aGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTc                     <  1:2745046/69‑1 (MQ=255)                                 aGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTc                     <  1:2288081/69‑1 (MQ=255)                                 aGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTc                     <  1:65681/69‑1 (MQ=255)                                 aGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTc                     <  1:66957/69‑1 (MQ=255)                                 aGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTc                     <  1:1270847/69‑1 (MQ=255)                                 aGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTc                     <  1:2468124/69‑1 (MQ=255)                                    ttCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTc                     <  1:3205675/66‑1 (MQ=255)                                    ttCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTc                     <  1:818511/66‑1 (MQ=255)                                    ttCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTc                     <  1:420067/66‑1 (MQ=255)                                    ttCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTc                     <  1:2596755/66‑1 (MQ=255)                                              gCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTCAACGCCAAACTTa        <  1:1155867/69‑1 (MQ=255)                                              gCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTCAACGCCAAACTTa        <  1:824095/69‑1 (MQ=255)                                                   ggCCTGATGCACATTTGGGTCCAGTGGGACGTTGGTTTCGGCGATCACTTCAACGCCAAACTTACGcac   <  1:3125463/69‑1 (MQ=255)                                                   ggCCTGATGCACATTTGGGTCCAGTGGGACGTTGGTTTCGGCGATCACTTCAACGCCAAACTTACGcac   <  1:1212332/69‑1 (MQ=255)                                                   ggCCTGATGCACATTTGGGTCCAGTGGGACGTTGGTTTCGGCGATCACTTCAACGCCAAACTTACGcac   <  1:2452701/69‑1 (MQ=255)                                                   ggCCTGATGCACATTTGGGTCCAGTGGGACGTTGGTTTCGGCGATCACTTCAACGCCAAACTTACGcac   <  1:971052/69‑1 (MQ=255)                                                   ggCCTGATGCACATTTGGGTCCAGTGGGACGTTGGTTTCGGCGATCACTTCAACGCCAAACTTACGcac   <  1:2172327/69‑1 (MQ=255)                                                   ggCCTGATGCACATTTGGGTCCAGTGGGACGTTGGTTTCGGCGATCACTTCAACGCCAAACTTACGcac   <  1:1522442/69‑1 (MQ=255)                                                   ggCCTGATGCACATTTGGGTCCAGTGGGACGTTGGTTTCGGCGATCACTTCAACGCCAAACTTACGcac   <  1:582142/69‑1 (MQ=255)                                                   ggCCTGATGCACATTTGGGTCCAGTGGGACGTTGGTTTCGGCGATCACTTCAACGCCAAACTTACGcac   <  1:1616274/69‑1 (MQ=255)                                                    gCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTCAACGCCAAACTTACGcaca  <  1:1182629/69‑1 (MQ=255)                                                    gCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTCAACGCCAAACTTACGcaca  <  1:2035377/69‑1 (MQ=255)                                                    gCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTCAACGCCAAACTTACGcaca  <  1:3002610/69‑1 (MQ=255)                                                    gCCTGATGAACATTTGGGTCCAGTGGGACGTTGGTTTCGGCGATCACTTCAACGCCAAACTTACGcac   <  1:838971/68‑1 (MQ=255)                                                      cTGATGCACATTTGGGTCCAGTGGGACGTTGGTTTCGGCGATCACTTCAACGCCAAACTTACGcac   <  1:1187919/66‑1 (MQ=255)                                                                  cGGGTCCAGTGGGATGTTAGTTTCGGCGATCACTTCAACGCCAAACt          <  1:1073049/47‑1 (MQ=255)                                                                  cGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTCAACGCCAAACt          <  1:972126/47‑1 (MQ=255)                                                                  cGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTCAACGCCAAACt          <  1:1477334/47‑1 (MQ=255)                                                                  cGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTCAACGCCAAACt          <  1:450236/47‑1 (MQ=255)                                                                  cGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTCAACGCCAAACt          <  1:3322048/47‑1 (MQ=255)                                                                  cGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTCAACGCCAAACt          <  1:1718974/47‑1 (MQ=255)                                                                  cGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTCAACGCCAAACt          <  1:2924612/47‑1 (MQ=255)                                                                  cGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTCAACGCCAAACt          <  1:2430783/47‑1 (MQ=255)                                                                  cGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTCAACGCCAAACt          <  1:2450929/47‑1 (MQ=255)                                                                  cGGCTCCAGTGGGACGTTAGTTTCGGCGATCACTTCAACGCCAAACt          <  1:166112/47‑1 (MQ=255)                                                                  |                                                       ATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTCCACCATTGCGATGGCCTGATGCACATTCGGGTCCAGTGGGACGTTAGTTTCGGCGATCACTTCAACGCCAAACTTACGCACA  >  minE/1782877‑1782996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑