| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,784,663 | C→T | 46.1% | R61R (CGC→CGT) | recN → | recombination and repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,784,663 | 0 | C | T | 46.1% | 2.2 / 29.5 | 39 | R61R (CGC→CGT) | recN | recombination and repair protein |
| Reads supporting (aligned to +/- strand): ref base C (8/13); new base T (12/6); total (20/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTG > minE/1784595‑1784716 | aTAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTcgc > 1:3408462/1‑69 (MQ=255) tAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTcgcg > 1:3189758/1‑69 (MQ=255) cTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGcc > 1:1210108/1‑69 (MQ=255) tGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTAGCGGCGCTGCTCGCGCTGACCTGTGCGCCCGt > 1:2236047/1‑69 (MQ=255) tGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGt > 1:3115030/1‑69 (MQ=255) tGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGt > 1:3191433/1‑69 (MQ=255) tGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGt > 1:787604/1‑69 (MQ=255) tGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGt > 1:1204141/1‑69 (MQ=255) tGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGt > 1:2842824/1‑69 (MQ=255) ctcGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGtttt > 1:1350142/1‑69 (MQ=255) tggtCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTtct < 1:2687242/67‑1 (MQ=255) tggtCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTtct < 1:700271/67‑1 (MQ=255) tggtCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTtct < 1:1254086/67‑1 (MQ=255) tggtCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTtct < 1:599181/67‑1 (MQ=255) gcTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGa < 1:270239/68‑1 (MQ=255) gcTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGa < 1:2334057/68‑1 (MQ=255) gcTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGa < 1:3106304/68‑1 (MQ=255) gcTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGa < 1:1447034/68‑1 (MQ=255) gcTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGa < 1:1117874/68‑1 (MQ=255) gcTGAAGCAGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGa < 1:979544/68‑1 (MQ=255) aaGCCGACATGGTGCGTACCGGCGCTGCTCGTGCCGACCTGTGCGCCCGTTTTTCTCTg < 1:2061531/59‑1 (MQ=255) aaGCCGACATGGTGCGTACCGGCGCTGCTCGTGCCGACCTGTGCGCCCGTTTTTCTCTg < 1:558919/59‑1 (MQ=255) aaGCCGACATGGTGCGTACCGGCGCTGCTCGTGCCGACCTGTGCGCCCGTTTTTCTCTg < 1:1930049/59‑1 (MQ=255) aaGCCGACATGGTGCGTACCGGCGCTGCTCGTGCCGACCTGTGCGCCCGTTTTTCTCTg < 1:809984/59‑1 (MQ=255) aaGCCGACATGGTGCGTACCGGCGCTCCTCGTGCCGACCTGTGCGCCCGTTTTTCTCTg < 1:991031/59‑1 (MQ=255) tGCGTACCGGCGCTGCTCGTGCCGACCTGTGCGCCCGTTTTTCTCTg < 1:534887/47‑1 (MQ=39) gCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTgc < 1:2357288/68‑1 (MQ=255) gCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTgc < 1:1190964/68‑1 (MQ=255) cGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTgc < 1:2282749/67‑1 (MQ=255) tACCGGCGCTGCTCGTGCCGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGc > 1:1986173/1‑57 (MQ=255) tACCGGCGCTGCTCGTGCCGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCCCTgcgtt > 1:2630208/1‑66 (MQ=255) tACCGGCGCTGCTCGTGCCGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCCCTgcgtt > 1:1973951/1‑66 (MQ=255) tACCGGCGCTGCTCGTGCCGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCCCTgcgtt > 1:1157619/1‑66 (MQ=255) tACCGGCGCTGCTCGTGCCGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCCCTgcgt > 1:1395840/1‑66 (MQ=255) tACCGGCGCTGCTCGTGCCGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCCCTGCgttg > 1:3034394/1‑69 (MQ=255) tACCGGCGCTGCTCGTGCCGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCCCTGCgttg > 1:298527/1‑69 (MQ=255) tACCGGCGCTGCTCGTGCCGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCCCTGCgttg > 1:3425969/1‑69 (MQ=255) tACCGGCGCTGCTCGTGCCGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCCCTGCgttg > 1:401723/1‑69 (MQ=255) tACCGGCGCTGCTCGTGCCGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCCCTGCgttg > 1:503065/1‑69 (MQ=255) tACCGGCGCTGCTCGTGCCGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCCCTGCgttg > 1:1743723/1‑69 (MQ=255) tACCGGCGCTGCTCGTGCCGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCCCTGCgttg > 1:1500421/1‑69 (MQ=255) | ATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTG > minE/1784595‑1784716 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |