| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,787,112 | A→G | 100% | C113C (TGT→TGC) | yfjG ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,787,112 | 0 | A | G | 86.4% | 48.4 / 0.1 | 29 | C113C (TGT→TGC) | yfjG | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/4); new base G (0/25); total (0/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACACGCGACCAAAGGCGAGTTCAATCAACTTATTGGTAAACTCAAAGTCGAGATGAAACTCGATACGACACGCCTCCTGGCTCAGCGGCGTAAACTTCCATCCACCAATCAATTTCTTGAACGGCCCATCC > minE/1787045‑1787175 | acacGCGACCAAAGGCGAGTTCAATCAACTTATTGGTAAACTCAAAGTCGAGATGAAACTCGATACGa < 1:955914/68‑1 (MQ=255)acacGCGACCAAAGGCGAGTTCAATCAACTTATTGGTAAACTCAAAGTCGAGATGAAACTCGATACGa < 1:2010653/68‑1 (MQ=255)acacGCGACCAAAGGCGAGTTCAATCAACTTATTGGTAAACTCAAAGTCGAGATGAAACTCGATACGa < 1:1474766/68‑1 (MQ=255)acacGCGACCAAAGGCGAGTTCAATCAACTTATTGGTAAACTCAAAGTCGAGATGAAACTCGATACGa < 1:1585770/68‑1 (MQ=255)acacGCGACCAAAGGCGAGTTCAATCAACTTATTGGTAAACTCAAAGTCGAGATGAAACTCGATACGa < 1:366819/68‑1 (MQ=255) cacGCGACCAAAGGCGAGTTCAATCAACTTATTGGTAAACTCAAAGTCGAGATGAAACTCGATACGa < 1:1976063/67‑1 (MQ=255) cgACCAAAGGCGAGTTCAATCAACTTATTGGTAAACTCAAAGTCGAGATGAAACTCGATACGACACGcc < 1:2429204/69‑1 (MQ=255) gACCAAAGGCGAGTTCAATCAACTTATTGGTAAACTCAAAGTCGAGATGAAACTCAATACGGCACGcgt < 1:606037/69‑3 (MQ=255) gACCAAAGGCGAGTTCAATCAACTTATTGGTAAACTCAAAGTCGAGATGAAACTCAATACGGCACGcgt < 1:2030783/69‑3 (MQ=255) gACCAAAGGCGAGTTCAATCAACTTATTGGTAAACTCAAAGTCGAGATGAAACTCAATACGGCACGcgt < 1:253911/69‑3 (MQ=255) gACCAAAGGCGAGTTCAATCAACTTATTGGTAAACTCAAAGTCGAGATGAAACTCAATACGGCACGcgt < 1:527929/69‑3 (MQ=255) gACCAAAGGCGAGTTCAATCAACTTATTGGTAAACTCAAAGTCGAGATGAAACTCAATACGGCACGcgt < 1:1452091/69‑3 (MQ=255) gACCAAAGGCGAGTTCAATCAACTTATTGGTAAACTCAAAGTCGAGATGAAACTCAATACGGCACGcgt < 1:901899/69‑3 (MQ=255) tcaatcaaCTTATTGGTAAACTCAAAGTCGAGATGAAACTCAATACGGCACGcgt < 1:1723902/55‑3 (MQ=255) aaCTTATTGGTAAACTCAAAGTCGAGATGAAACTCGATACGa < 1:1285046/42‑1 (MQ=255) cTCAAAGTCGAGATGAAACTCGATACGACACGCCTCCTGGCTCAGCGGCGTAAACTtc < 1:2072236/58‑1 (MQ=255) cTCAAAGTCGAGATGAAACTCGATACGACACGCCTCCTGGCTCAGCGGCGTAAACTtc < 1:888123/58‑1 (MQ=255) cTCAAAGTCGAGATGAAACTCGATACGACACGCCTCCTGGCTCAGCGGCGTAAACTtc < 1:1644570/58‑1 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:504180/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:968087/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:1366421/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:1416566/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:1504343/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:563758/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:1656048/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:511273/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:2018156/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:474017/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:1756669/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:3159116/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:1862747/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:1887331/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:2407821/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:2184895/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:1002874/68‑2 (MQ=255) aCGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:572727/67‑2 (MQ=255) | ACACGCGACCAAAGGCGAGTTCAATCAACTTATTGGTAAACTCAAAGTCGAGATGAAACTCGATACGACACGCCTCCTGGCTCAGCGGCGTAAACTTCCATCCACCAATCAATTTCTTGAACGGCCCATCC > minE/1787045‑1787175 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |