| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,787,118 | C→G | 100% | E111D (GAG→GAC) | yfjG ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,787,118 | 0 | C | G | 85.8% | 40.9 / 0.6 | 21 | E111D (GAG→GAC) | yfjG | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/3); new base G (0/18); total (0/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGACCAAAGGCGAGTTCAATCAACTTATTGGTAAACTCAAAGTCGAGATGAAACTCGATACGACACGCCTCCTGGCTCAGCGGCGTAAACTTCCATCCACCAATCAATTTCTTGAACGGCCCATCC > minE/1787050‑1787175 | cgACCAAAGGCGAGTTCAATCAACTTATTGGTAAACTCAAAGTCGAGATGAAACTCGATACGACACGcc < 1:2429204/69‑1 (MQ=255) cTCAAAGTCGAGATGAAACTCGATACGACACGCCTCCTGGCTCAGCGGCGTAAACTtc < 1:888123/58‑1 (MQ=255) cTCAAAGTCGAGATGAAACTCGATACGACACGCCTCCTGGCTCAGCGGCGTAAACTtc < 1:1644570/58‑1 (MQ=255) cTCAAAGTCGAGATGAAACTCGATACGACACGCCTCCTGGCTCAGCGGCGTAAACTtc < 1:2072236/58‑1 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:2407821/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:968087/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:563758/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:511273/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:504180/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:474017/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:3159116/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:1002874/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:2184895/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:2018156/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:1887331/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:1862747/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:1756669/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:1656048/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:1504343/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:1416566/68‑2 (MQ=255) tACGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:1366421/68‑2 (MQ=255) aCGGCACGCGTCCTGGCTCAACGGCGTAAACTTCCAACCGCCGATCAATTTCTTAAACGGTCCATca < 1:572727/67‑2 (MQ=255) cctcctGGCTCAGCGGCGTAAACTTCCATCCACCAATCAATTTCTTg > 1:1112811/1‑47 (MQ=255) cctcctGGCTCAGCGGCGTAAACTTCCATCCACCAATCAATTTCTTg > 1:2033980/1‑47 (MQ=255) cctcctGGCTCAGCGGCGTAAACTTCCATCCACCAATCAATTTCTTg > 1:1311347/1‑47 (MQ=255) cctcctGGCTCAGCGGCGTAAACTTCCATCCACCAATCAATTTCTTg > 1:883079/1‑47 (MQ=255) cctcctGGCTCAGCGGCGTAAACTTCCATCCACCAATCAATTTCTTg > 1:656800/1‑47 (MQ=255) cctcctGGCTCAGCGGCGTAAACTTCCATCCACCAATCAATTTCTTg > 1:572071/1‑47 (MQ=255) cctcctGGCTCAGCGGCGTAAACTTCCATCCACCAATCAATTTCTTg > 1:153240/1‑47 (MQ=255) cctcctGGCTCAGCGGCGTAAACTTCCATCCACCAATCAATTTCTTg > 1:3233047/1‑47 (MQ=255) cctcctGGCTCAGCGGCGTAAACTTCCATCCACCAATCAATTTCTTg > 1:2973959/1‑47 (MQ=255) cctcctGGCTCAGCGGCGTAAACTTCCATCCACCAATCAATTTCTTg > 1:2689884/1‑47 (MQ=255) | CGACCAAAGGCGAGTTCAATCAACTTATTGGTAAACTCAAAGTCGAGATGAAACTCGATACGACACGCCTCCTGGCTCAGCGGCGTAAACTTCCATCCACCAATCAATTTCTTGAACGGCCCATCC > minE/1787050‑1787175 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |