| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,790,943 | C→T | 44.9% | I263I (ATC→ATT) | gabD → | succinate‑semialdehyde dehydrogenase I, NADP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,790,943 | 0 | C | T | 44.9% | 13.9 / 53.7 | 69 | I263I (ATC→ATT) | gabD | succinate‑semialdehyde dehydrogenase I, NADP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (31/7); new base T (18/13); total (49/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.77e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01 | |||||||||||
GAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGC > minE/1790881‑1791003 | gAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCttt > 1:818765/1‑69 (MQ=255)gAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCttt > 1:1243871/1‑69 (MQ=255)gAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCttt > 1:445960/1‑69 (MQ=255)gAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCttt > 1:3374374/1‑69 (MQ=255)gAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCttt > 1:32485/1‑69 (MQ=255)gAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCttt > 1:1508329/1‑69 (MQ=255)gAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCttt > 1:3008204/1‑69 (MQ=255)gAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCttt > 1:2961302/1‑69 (MQ=255)gAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCttt > 1:2188829/1‑69 (MQ=255)gAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCttt > 1:1848769/1‑69 (MQ=255) cAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGAc > 1:1086978/1‑69 (MQ=255) cAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGAc < 1:739734/69‑1 (MQ=255) cAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGAc > 1:1358848/1‑69 (MQ=255) cAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGAc > 1:1626798/1‑69 (MQ=255) cAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGAc > 1:2044673/1‑69 (MQ=255) cAGTGCGCGAAAGACATAAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGAc < 1:2748442/69‑1 (MQ=255) aGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGAc < 1:1796792/68‑1 (MQ=255) tGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGAt > 1:2747708/1‑69 (MQ=255) tGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGAt > 1:1713926/1‑69 (MQ=255) tGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGAt > 1:226912/1‑69 (MQ=255) tGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGAt > 1:2635783/1‑69 (MQ=255) cgAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGcc < 1:2490223/68‑1 (MQ=255) gAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGcc < 1:576542/67‑1 (MQ=255) tCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGaa > 1:286710/1‑69 (MQ=255) tCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGaa > 1:829803/1‑69 (MQ=255) tCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGaa > 1:1570853/1‑69 (MQ=255) tCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGaa > 1:1160102/1‑69 (MQ=255) tCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGaa > 1:1460439/1‑69 (MQ=255) tCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGaa > 1:1180497/1‑69 (MQ=255) tCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGa > 1:845870/1‑68 (MQ=255) cGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGAc < 1:355110/41‑1 (MQ=255) tGGAGCTGGGCGGCTACGCACCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGgcg > 1:2097898/1‑69 (MQ=255) tGGAGCTGGGCGGCAACGCACCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGgcg > 1:625603/1‑69 (MQ=255) tGGAGCTGGGCGGCAACGCACCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGgcg > 1:1610090/1‑69 (MQ=255) tGGAGCTGGGCGGCAACGCACCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGgcg > 1:3042160/1‑69 (MQ=255) tGGAGCTGGGCGGCAACGCACCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGgcg > 1:806034/1‑69 (MQ=255) tGGAGCTGGGCGGCAACGCACCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGgcg > 1:2599106/1‑69 (MQ=255) tGGAGCTGGGCGGCAACGCACCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGgcg > 1:2504501/1‑69 (MQ=255) tGGAGCTGGGCGGCAACGCACCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGgcg > 1:975901/1‑69 (MQ=255) tGGAGCTGGGCGGCAACGCACCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGgcg > 1:1896726/1‑69 (MQ=255) ggAGCTGGGCGGTAACGCACCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGgcg > 1:393170/1‑68 (MQ=255) ggAGCTGGGCGGCAACGCACCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGgcgc > 1:770560/1‑69 (MQ=255) ggAGCTGGGCGGCAACGCACCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGgcgc > 1:2887420/1‑69 (MQ=255) ggAGCTGGGCGGCAACGCACCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGgcgc > 1:3118033/1‑69 (MQ=255) ggAGCTGGGCGGCAACGCACCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGgcgc > 1:3099176/1‑69 (MQ=255) ggAGCTGGGCGGCAACGCACCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGgcgc > 1:2379560/1‑69 (MQ=255) ggAGCTGGGCGGCAACGCACCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGgcgc > 1:919578/1‑69 (MQ=255) ggAGCTGGGCGGCAACGCACCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGgcg > 1:408654/1‑68 (MQ=255) ggAGCTGGGCGGCAACGCACCGTTTATTGTCTTTGACGATGCCGATCTAGATAAAGCCGTGGAAGgcgc > 1:1922696/1‑69 (MQ=255) aaCGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGaaa > 1:2921916/1‑69 (MQ=255) aaCGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGaaa > 1:3267708/1‑69 (MQ=255) aaCGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGaaa > 1:754711/1‑69 (MQ=255) aaCGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGaaa > 1:2591422/1‑69 (MQ=255) aaCGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGaaa > 1:2219268/1‑69 (MQ=255) aaCGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGaa > 1:1302270/1‑68 (MQ=255) cgcaCCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGGCGCGCTGGCCTCGAAAtt < 1:998311/69‑1 (MQ=255) cgcaCCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGGCGCGCTGGCCTCGAAAtt < 1:719934/69‑1 (MQ=255) cgcaCCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGGCGCGCTGGCCTCGAAAtt < 1:565898/69‑1 (MQ=255) cgcaCCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGGCGCGCTGGCCTCGAAAtt < 1:1392563/69‑1 (MQ=255) cgcaCCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGGCGCGCTGGCCTCGAAAtt < 1:3318204/69‑1 (MQ=255) cgcaCCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGGCGCGCTGGCCTCGAAAtt < 1:3272219/69‑1 (MQ=255) cgcaCCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGGCGCGCTGGCCTCGAAAtt < 1:307270/69‑1 (MQ=255) cgcaCCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGGCGCGCTGGCCTCGAAAtt < 1:2893443/69‑1 (MQ=255) cgcaCCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGGCGCGCTGGCCTCGAAAtt < 1:1030367/69‑1 (MQ=255) cgcaCCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGGCGCGCTGGCCTCGAAAtt < 1:2435857/69‑1 (MQ=255) cgcaCCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGGCGCGCTGGCCTCGAAAtt < 1:1924411/69‑1 (MQ=255) cgcaCCGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGGCGCCCTGGCCTCGAAAtt < 1:2152383/69‑1 (MQ=255) gcaccGTTTATTGTCTTTGACGATGCCGATCTCGATAAAGCCGTGGAAGGCGCGCTGGCCTCGAAAtt < 1:2294241/65‑1 (MQ=255) ccGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGc < 1:805299/69‑1 (MQ=255) | GAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATTCCGC > minE/1790881‑1791003 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |