| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,799,423 | C→T | 45.9% | F83F (TTC→TTT) | proW → | glycine betaine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,799,423 | 0 | C | T | 45.9% | 11.9 / 59.4 | 74 | F83F (TTC→TTT) | proW | glycine betaine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (17/23); new base T (13/21); total (30/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01 | |||||||||||
CCCGCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAATTGCTGCTGGG > minE/1799360‑1799482 | cccGCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCTTTCAg < 1:1328872/67‑1 (MQ=255)cccGCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCTTTCAg < 1:401891/67‑1 (MQ=255)cccGCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCTTTCAg < 1:2686485/67‑1 (MQ=255)cccGCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCTTTCAg < 1:48207/67‑1 (MQ=255)cccGCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCTTTCAg < 1:506369/67‑1 (MQ=255)cccGCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCTTTCAg < 1:1500843/67‑1 (MQ=255)cccGCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCTTTCAg < 1:2010604/67‑1 (MQ=255)cccGCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCTTTCAg < 1:2410659/67‑1 (MQ=255)cccGCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCTTTCAg < 1:778833/67‑1 (MQ=255)cccGCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCTTTCAg < 1:3210719/67‑1 (MQ=255)cccGCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCTTTCAg < 1:844553/67‑1 (MQ=255)cccGCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCTTTCAg < 1:2447540/67‑1 (MQ=255)cccGCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCTTTCAg < 1:84751/67‑1 (MQ=255)cccGCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCTTTCAg < 1:1053212/67‑1 (MQ=255)cccGCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCTTTCAg < 1:18247/67‑1 (MQ=255) cTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGt > 1:2362269/1‑68 (MQ=255) cTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGt > 1:2036069/1‑68 (MQ=255) cTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGt > 1:1175239/1‑68 (MQ=255) cTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGTg > 1:2587917/1‑69 (MQ=255) cTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGTg > 1:2341496/1‑69 (MQ=255) cTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGTg > 1:81080/1‑69 (MQ=255) cTCGACAGTTGCGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGTg > 1:1399301/1‑69 (MQ=255) gTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCTTTCAg < 1:71253/51‑1 (MQ=255) tGAAGGGATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATta < 1:92125/69‑1 (MQ=255) gAAGGGATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATtat > 1:2008206/1‑69 (MQ=255) gAAGGGATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATta < 1:445343/68‑1 (MQ=255) gAAGGGATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATta > 1:2355491/1‑68 (MQ=255) gggATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATtata < 1:2160746/67‑1 (MQ=255) gggATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATtata < 1:1360746/67‑1 (MQ=255) gggATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATtata < 1:2570272/67‑1 (MQ=255) gggTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGtt < 1:2145836/69‑1 (MQ=255) gggTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGtt < 1:1753158/69‑1 (MQ=255) gggTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGtt < 1:850141/69‑1 (MQ=255) gggTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCATCAACGGtt < 1:366456/69‑1 (MQ=255) cGTTACCCATTTTCGTCCCGTCTTTCAGGGCGTGCGCGTTCCGGTTGATTACATCCTCAACGGTTTCCa < 1:2840038/69‑1 (MQ=255) cGTTACCCATTTTCGTCCCGTCTTTCAGGGCGTGCGCGTTCCGGTTGATTACATCCTCAACGGTTTCCa < 1:3426235/69‑1 (MQ=255) cGTTACCCATTTTCGTCCCGTCTTTCAGGGCGTGCGCGTTCCGGTTGATTACATCCTCAACGGTTTCCa < 1:2070328/69‑1 (MQ=255) cGTTACCCATTTTCGTCCCGTCTTTCAGGGCGTGCGCGTTCCGGTTGATTACATCCTCAACGGTTTCCa < 1:1361933/69‑1 (MQ=255) cGTTACCCATTTTCGTCCCGTCTTTCAGGGCGTGCGCGTTCCGGTTGATTACATCCTCAACGGTTTCCa < 1:1231882/69‑1 (MQ=255) ttACCCATTTTCGTCCCGTCTTTCAGGGCGTGCGCGTTCCGGTTGATTACATCTTCAACGGTTTCCAGc > 1:256411/1‑69 (MQ=255) ttACCCATTTTCGTCCCGTCTTTCAGGGCGTGCGCGTTCCGGTTGATTACATCCTCAACGGTTTCCAg > 1:3154430/1‑68 (MQ=255) ttACCCATTTTCGTCCCGTCTTTCAGGGCGTGCGCGTTCCGGTTGATTACATCCTCAACGGTTTCCAGc > 1:381918/1‑69 (MQ=255) ttACCCATTTTCGTCCCGTCTTTCAGGGCGTGCGCGTTCCGGTTGATTACATCCTCAACGGTTTCCAGc > 1:1072470/1‑69 (MQ=255) ttACCCATTTTCGTCCCGTCTTTCAGGGCGTGCGCGTTCCGGTTGATTACATCCTCAACGGTTTCCAGc > 1:1267579/1‑69 (MQ=255) ttACCCATTTTCGTCCCGTCTTTCAGGGCGTGCGCGTTCCGGTTGATTACATCCTCAACGGTTTCCAGc > 1:1477595/1‑69 (MQ=255) ttACCCATTTTCGTCCCGTCTTTCAGGGCGTGCGCGTTCCGGTTGATTACATCCTCAACGGTTTCCAGc > 1:1726204/1‑69 (MQ=255) ttACCCATTTTCGTCCCGTCTTTCAGGGCGTGCGCGTTCCGGTTGATTACATCCTCAACGGTTTCCAGc > 1:1773373/1‑69 (MQ=255) ttACCCATTTTCGTCCCGTCTTTCAGGGCGTGCGCGTTCCGGTTGATTACATCCTCAACGGTTTCCAGc > 1:3267939/1‑69 (MQ=255) ttACCCATTTTCGTCCCGTCTTTCAGGGCGTGCGCGTTCCGGTTGATTACATCCTCAACGGTTTCCAGc > 1:1784708/1‑69 (MQ=255) ttACCCATTTTCGTCCCGTCTTTCAGGGCGTGCGCGTTCCGGTTGATTACATCCTCAACGGTTTCCAGc > 1:3130476/1‑69 (MQ=255) ttACCCATTTTCGTCCCGTCTTTCAGGGCGTGCGCGTTCCGGTTGATTACATCCTCAACGGTTTCCAGc > 1:2966784/1‑69 (MQ=255) ttACCCATTTTCGTCCCGTCTTTCAGGGCGTGCGCGTTCCGGTTGATTACATCCTCAACGGTTTCCAGc > 1:197805/1‑69 (MQ=255) ccATTTCCGTCCCGTCTTCCAGGGGGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAAt < 1:3155063/68‑1 (MQ=255) ccATTTCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAAt < 1:2151737/68‑1 (MQ=255) ccATTTCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAAt < 1:1294745/68‑1 (MQ=255) ccATTTCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAAt < 1:1295739/68‑1 (MQ=255) ccATTTCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAAt < 1:431994/68‑1 (MQ=255) ccATTTCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAAt < 1:1657711/68‑1 (MQ=255) ccATTTCCGTCCCGTCTTCCAGGGCGTGCGCGGTCCGGTTGATTATATCCTCAACGGTTTCCAGCAAt < 1:3260701/68‑1 (MQ=255) tCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAATtgctgc > 1:2643039/1‑69 (MQ=255) tCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAATtgctgc > 1:1229880/1‑69 (MQ=255) tCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAATtgctgc > 1:2711696/1‑69 (MQ=255) tCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAATtgctgc > 1:1736385/1‑69 (MQ=255) tCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAATtgctgc > 1:178044/1‑69 (MQ=255) tCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAATtgctgc > 1:3258793/1‑69 (MQ=255) tCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAATtgctg > 1:3022893/1‑68 (MQ=255) tCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAATtgctcc > 1:2624381/1‑67 (MQ=255) tcccgtcTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAATTGCTGCTggg < 1:743315/69‑1 (MQ=255) tcccgtcTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAATTGCTGCTggg < 1:1192398/69‑1 (MQ=255) tcccgtcTTCCAGGGCGTGCGCGGTCCGGTTGATTATATCCTCAACGGTTTCCAGCAATTGCTGCTggg < 1:3062834/69‑1 (MQ=255) cccgtcTTCCAGGGCTTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGc < 1:601407/55‑1 (MQ=255) cccgtcTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGc < 1:453319/55‑1 (MQ=255) cccgtcTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGc < 1:1605890/55‑1 (MQ=255) tCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGtt < 1:906136/42‑1 (MQ=255) | CCCGCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCCAGGGCGTGCGCGTTCCGGTTGATTATATCCTCAACGGTTTCCAGCAATTGCTGCTGGG > minE/1799360‑1799482 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |