| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,800,617 | G→A | 44.4% | G107G (GGG→GGA) | proX → | glycine betaine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,800,617 | 0 | G | A | 44.4% | 11.7 / 39.5 | 54 | G107G (GGG→GGA) | proX | glycine betaine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (21/9); new base A (6/18); total (27/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01 | |||||||||||
CGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAAC > minE/1800556‑1800664 | cGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGAGTATTTg < 1:449007/69‑1 (MQ=255)cGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGAGTATTTg < 1:2699879/69‑1 (MQ=255)cGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGAGTATTTg < 1:2184261/69‑1 (MQ=255)cGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGAGTATTTg < 1:3159213/69‑1 (MQ=255)cGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGAGTATTTg < 1:2057369/69‑1 (MQ=255)cGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGAGTATTTg < 1:1918121/69‑1 (MQ=255)cGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGAGTATTTg < 1:3376781/69‑1 (MQ=255)cGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGAGTATTTg < 1:1848543/69‑1 (MQ=255)cGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGAGTATTTg < 1:430900/69‑1 (MQ=255)cGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGAGTATTTg < 1:1702652/69‑1 (MQ=255)cGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGAGTATTTg < 1:157810/69‑1 (MQ=255)cGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGAGTATTTg < 1:710335/69‑1 (MQ=255)cGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGAGTATTTg < 1:1435783/69‑1 (MQ=255)cGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGAGTATTTg < 1:1426715/69‑1 (MQ=255)cGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGAGTATTTg < 1:1392423/69‑1 (MQ=255)cGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGAGTATTTg < 1:1388989/69‑1 (MQ=255)cGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGAGTATTTg < 1:1198135/69‑1 (MQ=255)cGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGAGTATTTg < 1:1252581/69‑1 (MQ=255) cATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgc > 1:1120574/1‑69 (MQ=255) cATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgc > 1:1443017/1‑69 (MQ=255) cATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgc > 1:1060707/1‑69 (MQ=255) cATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgc > 1:2177993/1‑69 (MQ=255) cATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgc > 1:2171085/1‑69 (MQ=255) cATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgc > 1:829952/1‑69 (MQ=255) cATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgc > 1:1779817/1‑69 (MQ=255) cATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgc > 1:795579/1‑69 (MQ=255) cATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgc > 1:639617/1‑69 (MQ=255) acaacaTGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGtt < 1:3330142/59‑1 (MQ=255) acaTGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgcgc > 1:3219398/1‑64 (MQ=255) acaTGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgcgc > 1:825303/1‑64 (MQ=255) acaTGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgcgc > 1:2778344/1‑64 (MQ=255) acaTGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgcgc > 1:933959/1‑64 (MQ=255) acaTGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgcgc > 1:1510933/1‑64 (MQ=255) acaTGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgcgc > 1:1633733/1‑64 (MQ=255) acaTGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGgcgc > 1:1958276/1‑64 (MQ=255) caTGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTa < 1:1140405/49‑1 (MQ=255) caTGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTa < 1:1399744/49‑1 (MQ=255) caTGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTa < 1:72497/49‑1 (MQ=255) caTGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTa < 1:2825332/49‑1 (MQ=255) caTGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTa < 1:3013631/49‑1 (MQ=255) caTGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTa < 1:2116546/49‑1 (MQ=255) caTGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTa < 1:1785900/49‑1 (MQ=255) caTGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTa < 1:3384776/49‑1 (MQ=255) ggCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCgg > 1:1849323/1‑46 (MQ=255) ggCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCgg > 1:2159578/1‑46 (MQ=255) ggCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCgg > 1:2375329/1‑46 (MQ=255) ggCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCgg > 1:2686363/1‑46 (MQ=255) ggCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCgg > 1:2552583/1‑46 (MQ=255) tAAGAAATTTTATCGTGAAGGAGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAAc > 1:2407320/1‑69 (MQ=255) tAAGAAATTTTATCGTGAAGGAGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAAc > 1:1981721/1‑69 (MQ=255) tAAGAAATTTTATCGTGAAGGAGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAAc > 1:1603316/1‑69 (MQ=255) tAAGAAATTTTATCGTGAAGGAGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAAc > 1:2745288/1‑69 (MQ=255) tAAGAAATTTTATCGTGAAGGAGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAAc > 1:138219/1‑69 (MQ=255) tAAGAAATTTTATCGTGAAGGAGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAAc > 1:1331914/1‑69 (MQ=255) | CGCCACTGCATGACAACATGTACGAAGCTGCCGGTGGCGATAAGAAATTTTATCGTGAAGGGGTATTTGTTAACGGCGCGGCACAGGGTTACCTGATCGATAAGAAAAC > minE/1800556‑1800664 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |