| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,801,836 | G→A | 51.5% | pseudogene (60/882 nt) | ygaY → | ECK2675:JW5428:b2681; predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,801,836 | 0 | G | A | 51.5% | ‑3.7 / 59.0 | 66 | pseudogene (60/882 nt) | ygaY | ECK2675:JW5428:b2681; predicted transporter |
| Reads supporting (aligned to +/- strand): ref base G (25/7); new base A (12/22); total (37/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.64e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
TCCTCGGTACGGCATTAACCGGTTTATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGATAAACGCGGCAAAGTGGTTGGCACTATTATG > minE/1801781‑1801893 | tCCTCGGTACGGCATTAACCGGTTTATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGc > 1:1795932/1‑69 (MQ=255)tCCTCGGTACGGCATTAACCGGTTTATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGc > 1:1792003/1‑69 (MQ=255) ccTCGGTACGGCATTAACCGGTTTATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGc > 1:2430538/1‑68 (MQ=255) cTCGGTACGGCATTAACCGGTTTATTCTCAGTCGTGGCACAAATTTTGGTTCCGCTGGCAGCGACGCTg < 1:2239142/69‑1 (MQ=255) cTCGGTACGGCATTAACCGGTTTATTCTCAGTCGTGGCACAAATTTTGGTTCCGCTGGCAGCGACGCTg < 1:2236341/69‑1 (MQ=255) cTCGGTACGGCATTAACCGGTTTATTCTCAGTCGTGGCACAAATTTTGGTTCCGCTGGCAGCGACGCTg < 1:3072587/69‑1 (MQ=255) cTCGGTACGGCATTAACCGGTTTATTCTCAGTCGTGGCACAAATTTTGGTTCCGCTGGCAGCGACGCTg < 1:1723951/69‑1 (MQ=255) cTCGGTACGGCATTAACCGGTTTATTCTCAGTCGTGGCACAAATTTTGGTTCCGCTGGCAGCGACGCTg < 1:3192217/69‑1 (MQ=255) ttAACCGGTTTATTCTCAGTCGTGGCACAAATTCTTGTTCCGCTGGCAGCGACGCTGGCTTCACCGGa > 1:92693/1‑68 (MQ=255) ttAACCGGTTTATTCTCAGTCGTGGCACAAATTCTGGTTCCGc > 1:2617511/1‑43 (MQ=255) ttAACCGGTTTATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGa > 1:1890403/1‑68 (MQ=255) ttAACCGGTTTATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGa > 1:2626726/1‑68 (MQ=255) ttAACCGGTTTATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGa > 1:2168024/1‑68 (MQ=255) ttAACCGGTTTATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGa > 1:3130967/1‑68 (MQ=255) ttAACCGGTTTATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGa > 1:3411765/1‑68 (MQ=255) ttAACCGGTTTATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGa > 1:1003479/1‑68 (MQ=255) ttAACCGGTTTATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGa > 1:474063/1‑68 (MQ=255) ttAACCGGTTTATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGa > 1:458035/1‑68 (MQ=255) ttAACCGGTTTATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGa > 1:1777268/1‑68 (MQ=255) ttAACCGGTTTATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGa > 1:3194572/1‑68 (MQ=255) ttAACCGGTTTATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGa > 1:1765622/1‑68 (MQ=255) ttAACCGGTTTATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGa > 1:1772915/1‑68 (MQ=255) ttAACCGGTTTATTATCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGa > 1:3055930/1‑68 (MQ=255) aaCCGGTTTATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGATaa > 1:2942535/1‑69 (MQ=255) aaCCGGTTTATTCACAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGATaa > 1:716373/1‑69 (MQ=255) tATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCg > 1:2212413/1‑56 (MQ=255) tATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCg > 1:3415434/1‑56 (MQ=255) tATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCg > 1:2903339/1‑56 (MQ=255) ctcAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGATAAACGCGGCAAAGt > 1:2128145/1‑69 (MQ=255) ctcAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGATAAACGCGGCAAAGt > 1:3175326/1‑69 (MQ=255) tcAGTCGTGGCACAAATTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTg > 1:3256070/1‑69 (MQ=255) tcAGTCGTGGCACAAATTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTg > 1:1185044/1‑69 (MQ=255) tcAGTCGTGGCACAAATTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTg > 1:2867646/1‑69 (MQ=255) tcAGTCGTGGCACAAATTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTg > 1:2772185/1‑69 (MQ=255) tcAGTCGTGGCACAAATTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTg > 1:2488034/1‑69 (MQ=255) tcAGTCGTGGCACAAATTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTg > 1:1663479/1‑69 (MQ=255) tcAGTCGTGGCACAAATTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTg > 1:242108/1‑69 (MQ=255) tcAGTCGTGGCACAAATTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTg > 1:1666536/1‑69 (MQ=255) tcAGTCGTGGCACAAATTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTg > 1:1944569/1‑69 (MQ=255) tcAGTCGTGGCACAAATTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTg > 1:2084965/1‑69 (MQ=255) tcAGTCGTGGCACAAATTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTg > 1:1996968/1‑69 (MQ=255) tcAGTCGTGGCAAAAATTCTGGTTCCACTGGAAGCGAc > 1:3187530/1‑38 (MQ=37) ggCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGATAAACGCGGCAAAGTGGTTGGCAc < 1:1238583/69‑1 (MQ=255) acaAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGATAAACGCGGCAAAGTGGTTGGCACtat < 1:1426788/69‑1 (MQ=255) aTTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGGGGCAAAGTGGTTGGCACCATTATg < 1:2539747/69‑1 (MQ=255) aTTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:2691172/69‑1 (MQ=255) aTTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:936019/69‑1 (MQ=255) aTTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:2528653/69‑1 (MQ=255) aTTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:1518807/69‑1 (MQ=255) aTTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:468152/69‑1 (MQ=255) aTTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:370835/69‑1 (MQ=255) aTTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:3244070/69‑1 (MQ=255) aTTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:3220015/69‑1 (MQ=255) aTTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:286221/69‑1 (MQ=255) aTTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:2411668/69‑1 (MQ=255) aTTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:1942151/69‑1 (MQ=255) aTTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:2248677/69‑1 (MQ=255) aTTCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGAACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:3366063/69‑1 (MQ=255) aTTCTGGTTCCACTGGCAGCGACGCTGGCCTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:1536295/69‑1 (MQ=255) ttCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:325080/68‑1 (MQ=255) ttCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:325271/68‑1 (MQ=255) ttCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:1636301/68‑1 (MQ=255) ttCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:1532214/68‑1 (MQ=255) ttCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:1855796/68‑1 (MQ=255) ttCTGGTTCCACTGGCAGCGACGCTGGCTTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:2434563/68‑1 (MQ=255) tCTGGTTCCACTGGCAGCGTCGCTGGCTTCACCGGACAAACGCGGCAAAGTGGTTGGCACCATTATg < 1:2069092/67‑1 (MQ=255) | TCCTCGGTACGGCATTAACCGGTTTATTCTCAGTCGTGGCACAAATTCTGGTTCCGCTGGCAGCGACGCTGGCTTCACCGGATAAACGCGGCAAAGTGGTTGGCACTATTATG > minE/1801781‑1801893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |