| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,802,088 | T→C | 48.4% | pseudogene (312/882 nt) | ygaY → | ECK2675:JW5428:b2681; predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,802,088 | 0 | T | C | 48.4% | ‑2.9 / 56.6 | 64 | pseudogene (312/882 nt) | ygaY | ECK2675:JW5428:b2681; predicted transporter |
| Reads supporting (aligned to +/- strand): ref base T (14/19); new base C (15/16); total (29/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
TGGTCTGCCACAAATGAAATCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATAAAATTCTGCGTACCCGCGCGTTGCTGGGCTGCCTGACCTTTGCCAATTTCAG > minE/1802022‑1802156 | tGGTCTGCCACAAATGAAATCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTAt > 1:2568462/1‑69 (MQ=255)tGGTCTGCCACAAATGAAATCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTAt > 1:2883794/1‑69 (MQ=255) cTGCCACAAATGAAATCAGAAACCGACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGtt < 1:96618/68‑1 (MQ=255) cTGCCACAAATGAAATCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGtt < 1:111371/68‑1 (MQ=255) cTGCCACAAATGAAATCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGtt < 1:61405/68‑1 (MQ=255) caAATGAAATCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGc > 1:684074/1‑69 (MQ=255) caAATGAAATCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGc > 1:2901888/1‑69 (MQ=255) caAATGAAATCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGc > 1:1807790/1‑69 (MQ=255) gAAATCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATaa < 1:465103/69‑1 (MQ=255) gAAATCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATaa < 1:2458014/69‑1 (MQ=255) gAAATCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATaa < 1:1881254/69‑1 (MQ=255) gAAATCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATaa < 1:3361188/69‑1 (MQ=255) gAAATCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATaa < 1:425745/69‑1 (MQ=255) gAAATCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATaa < 1:157932/69‑1 (MQ=255) gAAATCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATaa < 1:799380/69‑1 (MQ=255) gAAATCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATaa < 1:1367618/69‑1 (MQ=255) aTCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATAAAAt < 1:1151616/69‑1 (MQ=255) aTCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATAAAAt < 1:594284/69‑1 (MQ=255) aTCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATAAAAt < 1:778500/69‑1 (MQ=255) aTCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATAAAAt < 1:235189/69‑1 (MQ=255) aTCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATAAAAt < 1:1438539/69‑1 (MQ=255) aTCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATAAAAt < 1:1445640/69‑1 (MQ=255) tCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATAAAAt < 1:3430170/68‑1 (MQ=255) cACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGCATGTTTATCAGCAATAAGATCCTGCGCAcc > 1:967647/1‑69 (MQ=255) cACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGCATGTTTATCAGCAATAAGATCCTGCGCAcc > 1:839852/1‑69 (MQ=255) cACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGCATGTTTATCAGCAATAAGATCCTGCGCAcc > 1:810909/1‑69 (MQ=255) cACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGCATGTTTATCAGCAATAAGATCCTGCGCAcc > 1:633429/1‑69 (MQ=255) cACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGCATGTTTATCAGCAATAAGATCCTGCGCAcc > 1:155404/1‑69 (MQ=255) cACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGCATGTTTATCAGCAATAAGATCCTGCGCAcc > 1:1758292/1‑69 (MQ=255) cACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGCATGTTTATCAGCAATAAGATCCTGCGCAcc > 1:392780/1‑69 (MQ=255) cACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGCATGTTTATCAGCAATAAGATCCTGCGCAcc > 1:1818858/1‑69 (MQ=255) cACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGCATGTTTATCAGCAATAAGATCCTGCGCAcc > 1:3268214/1‑69 (MQ=255) cACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGCATGTTTATCAGCAATAAGATCCTGCGCAcc > 1:3267990/1‑69 (MQ=255) cACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGCATGTTTATCAGCAATAAGATCCTGCGCAcc > 1:2990327/1‑69 (MQ=255) cACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGCATGTTTATCAGCAATAAGATCCTGCGCAcc > 1:1877747/1‑69 (MQ=255) cACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGCATGTTTATCAGCAATAAGATCCTGCGCAcc > 1:194237/1‑69 (MQ=255) cACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGCATGTTTATCAGCAATAAGATCCTGCGCAcc > 1:2505715/1‑69 (MQ=255) cACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGCATGTTTATCAGCAATAAGATCCTGCGCAc > 1:681787/1‑68 (MQ=255) tCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGtt < 1:2393522/40‑1 (MQ=255) aCCCATAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATAAAATTCTGCGTACCCGCGCGTTGc > 1:1891050/1‑69 (MQ=255) aCCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATAAAATTCTGCGt > 1:931964/1‑56 (MQ=255) aCCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATAAAATTCTGCGTACCcg > 1:703745/1‑61 (MQ=255) aCCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATAAAATTCTGCGTACCCGCGCGTTGc > 1:2938213/1‑69 (MQ=255) aCCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATAAAATTCTGCGTACCCGCGCGTTGc > 1:1776810/1‑69 (MQ=255) aCCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATAAAATTCTGCGTACCCGCGCGTTGc > 1:1235625/1‑69 (MQ=255) tgttgGGTTCCGTTTTCAGTATGTTTATCAGCGATAAAATTCTGCGTACCCGCGCGtt > 1:823902/1‑58 (MQ=255) tgttgGGTTCCGTTTTCAGTATGTTTATCAGCGATAAAATTCTGCGTACCCGCGCGtt > 1:495075/1‑58 (MQ=255) ccGTTTTCAGTATGTTTATCAGCGATAAAATTCTGAGTACCCGCGCGTTGCTGGGCTGCCTGACCttt > 1:2389815/1‑68 (MQ=255) ttttCAGCATGTTTATCAGCAATAAGATCCTGCGCACCCGCGCGTTGCTGGGCTGCCTGACCTTTGCCa < 1:2238308/69‑1 (MQ=255) ttttCAGCATGTTTATCAGCAATAAGATCCTGCGCACCCGCGCGTTGCTGGGCTGCCTGACCTTTGCCa < 1:2378866/69‑1 (MQ=255) ttttCAGCATGTTTATCAGCAATAAGATCCTGCGCACCCGCGCGTTGCTGGGCTGCCTGACCTTTGCCa < 1:96609/69‑1 (MQ=255) ttttCAGCATGTTTATCAGCAATAAGATCCTGCGCACCCGCGCGTTGCTGGGCTGCCTGACCTTTGCCa < 1:2318159/69‑1 (MQ=255) ttttCAGCATGTTTATCAGCAATAAGATCCTGCGCACCCGCGCGTTGCTGGGCTGCCTGACCTTTGCCa < 1:633522/69‑1 (MQ=255) ttttCAGCATGTTTATCAGCAATAAGATCCTGCGCACCCGCGCGTTGCTGGGCTGCCTGACCTTTGCCa < 1:2092755/69‑1 (MQ=255) ttttCAGCATGTTTATCAGCAATAAGATCCTGCGCACCCGCGCGTTGCTGGGCTGCCTGACCTTTGCCa < 1:1678090/69‑1 (MQ=255) ttttCAGCATGTTTATCAGCAATAAGATCCTGCGCACCCGCGCGTTGCTGGGCTGCCTGACCTTTGCCa < 1:516103/69‑1 (MQ=255) ttttCAGCATGTTTATCAGCAATAAGATCCTGCGCACCCGCGCGTTGCTGGGCTGCCTGACCTTTGCCa < 1:2499285/69‑1 (MQ=255) ttttCAGCATGTTTATCAGCAATAAGATCCTGCGCACCCGCGCGTTGCTGGGCTGCCTGACCTTTGCCa < 1:427932/69‑1 (MQ=255) ttttCAGCATGTTTATCAGCAATAAGATCCTGCGCACCCGCGCGTTGCTGGGCTGCCTGACCTTTGCCa < 1:2507393/69‑1 (MQ=255) ttttCAGCATGTTTATCAGCAATAAGATCCTGCGCACCCGCGCGTTGCTGGGCTGCCTGACCTTTGCCa < 1:2549184/69‑1 (MQ=255) ttttCAGCATGTTTATCAGCAATAAGATCCTGCGCACCCGCGCGTTGCTGGGCTGCCTGACCTTTGCCa < 1:3261902/69‑1 (MQ=255) ttttCAGCATGTTTATCAGCAATAAGATCCTGCGCACCCGCGCGTTGCTGGGCTGCCTGACCTTTGCCa < 1:2604011/69‑1 (MQ=255) tttCAGCATGTTTATCAGCAATAAGATCCTGCGCACCCGCGCGTTGCTGGGCTGCCTGACCTTTGCCa < 1:1383905/68‑1 (MQ=255) cAGCATGTTTATCAGCAATAAGATCCTGCGCACCCGCGCGTTGCTGGGCTGCCTGACCTTTGCCa < 1:2500797/65‑1 (MQ=255) tATGTTTATCAGCGATAAAATTCTGCGTACCCGCGCGTTGCTGGGCTGCCTGACCTTTGCCAATTTCAg < 1:2409653/69‑1 (MQ=255) | TGGTCTGCCACAAATGAAATCAGAAACCCACCTCAACTACCCACAGTTGTTGGGTTCCGTTTTCAGTATGTTTATCAGCGATAAAATTCTGCGTACCCGCGCGTTGCTGGGCTGCCTGACCTTTGCCAATTTCAG > minE/1802022‑1802156 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |