Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 137,466 C→A 20.5% E29D (GAG→GAT yadM ← predicted fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE137,4660CA20.5% 230.1 / 41.3 117E29D (GAG→GATyadMpredicted fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (20/72);  new base A (11/13);  total (31/86)
Fisher's exact test for biased strand distribution p-value = 3.57e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

ATCATTCGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCACTATTTTA  >  minE/137400‑137532
                                                                  |                                                                  
atcatTCGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAActc                                                                  <  1:2229505/69‑1 (MQ=255)
 tcatTCGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAActc                                                                  <  1:1599349/68‑1 (MQ=255)
  cgttCGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTa                                                                <  1:107384/67‑1 (MQ=255)
  catTCGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTa                                                                <  1:408743/69‑1 (MQ=255)
   atTCGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTa                                                                >  1:128416/1‑68 (MQ=255)
   atTCGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTAc                                                               >  1:2698261/1‑69 (MQ=255)
   atTCGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTAc                                                               >  1:429653/1‑69 (MQ=255)
   atTCGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTAc                                                               >  1:64330/1‑69 (MQ=255)
   atTCGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTAc                                                               >  1:2647122/1‑69 (MQ=255)
   atTCGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTAc                                                               >  1:1066546/1‑69 (MQ=255)
      cGTGACGTTATTGCCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTa                                                                >  1:2254913/1‑65 (MQ=255)
      cGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTa                                                                >  1:2376219/1‑65 (MQ=255)
      cGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTa                                                                >  1:1998354/1‑65 (MQ=255)
      cGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTa                                                                >  1:2745514/1‑65 (MQ=255)
      cGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTa                                                                >  1:2591186/1‑65 (MQ=255)
      cGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTa                                                                >  1:3005284/1‑65 (MQ=255)
      cGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTa                                                                >  1:3045384/1‑65 (MQ=255)
      cGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTa                                                                >  1:84810/1‑65 (MQ=255)
      cGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTa                                                                >  1:436775/1‑65 (MQ=255)
               attaCCAGTAAGTGTGATGTTGCAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGt                                                   >  1:2699380/1‑69 (MQ=255)
               attaCCAGTAAGTGTGATGTTGCAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGt                                                   >  1:1710300/1‑69 (MQ=255)
                ttaCCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAActc                                                                  <  1:2073568/53‑1 (MQ=255)
                     aGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCaaa                                             <  1:3408487/69‑1 (MQ=255)
                     aGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCaaa                                             <  1:2531649/69‑1 (MQ=255)
                     aGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCaaa                                             <  1:518944/69‑1 (MQ=255)
                     aGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCaaa                                             <  1:2701430/69‑1 (MQ=255)
                     aGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCaaa                                             <  1:1278801/69‑1 (MQ=255)
                     aGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCaaa                                             <  1:1756914/69‑1 (MQ=255)
                     aGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCaaa                                             <  1:3023352/69‑1 (MQ=255)
                     aGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCaaa                                             <  1:1345742/69‑1 (MQ=255)
                     aGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCaaa                                             <  1:3212496/69‑1 (MQ=255)
                     aGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCaaa                                             <  1:420866/69‑1 (MQ=255)
                     aGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCaaa                                             <  1:3354866/69‑1 (MQ=255)
                         aGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCg                                                    <  1:1131324/58‑1 (MQ=255)
                                       ggTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCa                                               <  1:908750/49‑1 (MQ=255)
                                       ggTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCa                                               <  1:1995748/49‑1 (MQ=255)
                                       ggTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCa                                               <  1:3093264/49‑1 (MQ=255)
                                       ggTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCa                                               <  1:2065052/49‑1 (MQ=255)
                                       ggTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCa                                               <  1:35634/49‑1 (MQ=255)
                                       ggTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGataataa                           <  1:695231/69‑1 (MQ=255)
                                       ggTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGataataa                           <  1:2505415/69‑1 (MQ=255)
                                       ggTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGataataa                           <  1:2509429/69‑1 (MQ=255)
                                       ggTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGataataa                           <  1:349147/69‑1 (MQ=255)
                                       ggTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGataataa                           <  1:2886700/69‑1 (MQ=255)
                                       ggTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGataataa                           <  1:2962078/69‑1 (MQ=255)
                                       ggTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGataataa                           <  1:129521/69‑1 (MQ=255)
                                       ggTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGataataa                           <  1:1683116/69‑1 (MQ=255)
                                       ggTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGataataa                           <  1:1972045/69‑1 (MQ=255)
                                        gTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGataataat                          <  1:2397726/69‑1 (MQ=255)
                                         ttgttgCTTTCACGGTAGCGGTAAAATCGACATTAACATCCGTTGCAAATACCGAAGGCGataacaata                         <  1:1619772/69‑1 (MQ=255)
                                         ttgttgCTTTCACGGTAGCGGTAAAATCGACATTAACATCCGTTGCAAATACCGAAGGCGataacaata                         <  1:937315/69‑1 (MQ=255)
                                         ttgttgCTTTCACGGTAGCGGTAAAATCGACATTAACATCCGTTGCAAATACCGAAGGCGataacaata                         <  1:1080708/69‑1 (MQ=255)
                                         ttgttgCTTTCACGGTAGCGGTAAAATCGACATTAACATCCGTTGCAAATACCGAAGGCGataacaata                         <  1:1202167/69‑1 (MQ=255)
                                         ttgttgCTTTCACGGTAGCGGTAAAATCGACATTAACATCCGTTGCAAATACCGAAGGCGataacaata                         <  1:1205987/69‑1 (MQ=255)
                                         ttgttgCTTTCACGGTAGCGGTAAAATCGACATTAACATCCGTTGCAAATACCGAAGGCGataacaata                         <  1:1394993/69‑1 (MQ=255)
                                         ttgttgCTTTCACGGTAGCGGTAAAATCGACATTAACATCCGTTGCAAATACCGAAGGCGataacaata                         <  1:1453248/69‑1 (MQ=255)
                                         ttgttgCTTTCACGGTAGCGGTAAAATCGACATTAACATCCGTTGCAAATACCGAAGGCGataacaata                         <  1:1615784/69‑1 (MQ=255)
                                         ttgttgCTTTCACGGTAGCGGTAAAATCGACATTAACATCCGTTGCAAATACCGAAGGCGataacaata                         <  1:203971/69‑1 (MQ=255)
                                         ttgttgCTTTCACGGTAGCGGTAAAATCGACATTAACATCCGTTGCAAATACCGAAGGCGataacaata                         <  1:2622919/69‑1 (MQ=255)
                                          tGTTGCTTTCACGGTAGCGGTAAAATCGACATTAACATCCGTTGCAAATACCGAAGGCGataacaatag                        >  1:1509424/1‑68 (MQ=255)
                                          tGTTGCTTTCACGGTAGCGGTAAAATCGACATTAACATCCGTTGCAAATACCGAAGGCGataacaatag                        >  1:154005/1‑68 (MQ=255)
                                          tGTTGCTTTCACGGTAGCGGTAAAATCGACATTAACATCCGTTGCAAATACCGAAGGCGataacaatag                        >  1:1965081/1‑68 (MQ=255)
                                          tGTTGCTTTCACGGTAGCGGTAAAATCGACATTAACATCCGTTGCAAATACCGAAGGCGataacaatag                        >  1:2081026/1‑68 (MQ=255)
                                          tGTTGCTTTCACGGTAGCGGTAAAATCGACATTAACATCCGTTGCAAATACCGAAGGCGataacaatag                        >  1:3147849/1‑68 (MQ=255)
                                          tGTTGCTTTCACGGTAGCGGTAAAATCGACATTAACATCCGTTGCAAATACCGAAGGCGataacaatag                        >  1:36002/1‑68 (MQ=255)
                                          tGTTGCTTTCACGGTAGCGGTAAAATCGACATTAACATCCGTTGCAAATACCGAAGGCGataacaatag                        >  1:57643/1‑68 (MQ=255)
                                          tGTTGCTTTCACGGTAGCGGTAAAATCGACATTAACATCCGTTGCAAATACCGAAGGCGataacaatag                        >  1:2355059/1‑68 (MQ=255)
                                          tGTTGCTTTCACGGTAGCGGTAAAATCGACATTAACATCCGTTGCAAATACCGAAGGCGataacaatag                        >  1:17339/1‑68 (MQ=255)
                                           gTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATAtt                       >  1:1193080/1‑69 (MQ=255)
                                              gCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATAtt                       <  1:2031412/66‑1 (MQ=255)
                                              gCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATAtt                       <  1:1458436/66‑1 (MQ=255)
                                              gCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATAtt                       <  1:1294528/66‑1 (MQ=255)
                                              gCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATAtt                       <  1:2965373/66‑1 (MQ=255)
                                              gCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATAtt                       <  1:1790956/66‑1 (MQ=255)
                                              gCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATAtt                       <  1:1203936/66‑1 (MQ=255)
                                                 ttGACAGTGGCTGTAAATTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATc                 <  1:2727718/69‑1 (MQ=255)
                                                 ttGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATc                 <  1:1050993/69‑1 (MQ=255)
                                                 ttGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATc                 <  1:620649/69‑1 (MQ=255)
                                                 ttGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATc                 <  1:2582793/69‑1 (MQ=255)
                                                 ttGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATc                 <  1:2016070/69‑1 (MQ=255)
                                                    aCAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAg               <  1:1770254/68‑1 (MQ=255)
                                                    aCAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAg               <  1:3274234/68‑1 (MQ=255)
                                                     cAGTGGCTGTAAACTCTTCATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGt              <  1:1249801/68‑1 (MQ=255)
                                                     cAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGt              <  1:354407/68‑1 (MQ=255)
                                                     cAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGt              <  1:3258783/68‑1 (MQ=255)
                                                     cAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGt              <  1:3139786/68‑1 (MQ=255)
                                                     cAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGt              <  1:2252145/68‑1 (MQ=255)
                                                     cAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGt              <  1:301913/68‑1 (MQ=255)
                                                     cAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGt              <  1:762881/68‑1 (MQ=255)
                                                     cAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGt              <  1:2478274/68‑1 (MQ=255)
                                                         ggCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCAc         <  1:2979760/69‑1 (MQ=255)
                                                         ggCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCAc         <  1:2443371/69‑1 (MQ=255)
                                                         ggCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCAc         <  1:777085/69‑1 (MQ=255)
                                                         ggCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCAc         <  1:686867/69‑1 (MQ=255)
                                                         ggCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCAc         <  1:2809841/69‑1 (MQ=255)
                                                         ggCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCAc         <  1:1810045/69‑1 (MQ=255)
                                                         ggCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCAc         <  1:543667/69‑1 (MQ=255)
                                                         ggCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCAc         <  1:2136840/69‑1 (MQ=255)
                                                         ggCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGGGATAATAATATTCCCATCAGTATCAc         <  1:2746434/69‑1 (MQ=255)
                                                                aaCTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCACTATTTTa  <  1:2301480/69‑1 (MQ=255)
                                                                  |                                                                  
ATCATTCGTGACGTTATTACCAGTAAGTGTGATGTTACAGGTTGTCGCTTTGACAGTGGCTGTAAACTCTACATTAATATCCGTTGCAAATACTGAGGGTGATAATAATATTCCCATCAGTATCACTATTTTA  >  minE/137400‑137532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: