| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,806,641 | T→G | 100% | A287A (GCT→GCG) | emrB → | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,806,641 | 0 | T | G | 77.4% | 61.6 / 7.8 | 35 | A287A (GCT→GCG) | emrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (3/5); new base G (1/26); total (4/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.02e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGG > minE/1806575‑1806700 | tAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTAt > 1:3343722/1‑69 (MQ=255)tAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTAt > 1:179146/1‑69 (MQ=255) ttCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCtgctgc > 1:2927155/1‑67 (MQ=255) cATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAgt < 1:888452/68‑1 (MQ=255) cATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAgt < 1:2844540/68‑1 (MQ=255) cATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAgt < 1:3115597/68‑1 (MQ=255) cATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAgt < 1:808685/68‑1 (MQ=255) cATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAgt < 1:829266/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:659010/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:351590/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:729407/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:791158/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:314547/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:3021056/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:2542426/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:2740791/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:1027944/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:1784382/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:113076/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:128157/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:1310191/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:1341728/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:1559605/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:1567260/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:2527267/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:1805481/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:1847038/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:196079/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:2019732/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:2045854/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:2154067/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:21774/68‑1 (MQ=255) ctctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGAGACGTg < 1:7652/68‑1 (MQ=255) tctACTTCGGCGCGATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTg < 1:495320/67‑1 (MQ=255) ttCGGCGCGATTGTTATGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACGTGGGCAgg > 1:349827/1‑68 (MQ=255) | TAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGG > minE/1806575‑1806700 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |