| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,807,079 | T→C | 35.1% | T433T (ACT→ACC) | emrB → | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,807,079 | 0 | T | C | 35.1% | 33.2 / 29.7 | 54 | T433T (ACT→ACC) | emrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (29/6); new base C (15/4); total (44/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGGCACGTCGATAACCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGTTCAACCCGAATGCCCAGGCGATGTACAGTCAACTGGAAGGGCTTGGG > minE/1807015‑1807142 | tCGGTACGTCCATAACGACGACGATGTGGACTAACCGCGAGTCGATGCACCATGCTCAGTTGACCGaat > 1:1685279/1‑67 (MQ=255)tCGGTACGTCCATAACGACGACGATGTGGACTAACCGCGAGTCGATGCACCATGCTCAGTTGACCGaat > 1:2034562/1‑67 (MQ=255)tCGGTACGTCCATAACGACGACGATGTGGACTAACCGCGAGTCGATGCACCATGCTCAGTTGACCGaat > 1:3080239/1‑67 (MQ=255)tCGGTACGTCCATAACGACGACGATGTGGACTAACCGCGAGTCGATGCACCATGCTCAGTTGACCGaat > 1:2046910/1‑67 (MQ=255)tCGGTACGTCCATAACGACGACGATGTGGACTAACCGCGAGTCGATGCACCATGCTCAGTTGACCGaat > 1:3280266/1‑67 (MQ=255)tCGGTACGTCCATAACGACGACGATGTGGACTAACCGCGAGTCGATGCACCATGCTCAGTTGACCGaat > 1:3021388/1‑67 (MQ=255)tCGGTACGTCCATAACGACGACGATGTGGACTAACCGCGAGTCGATGCACCATGCTCAGTTGACCGaat > 1:410887/1‑67 (MQ=255)tCGGTACGTCCATAACGACGACGATGTGGACTAACCGCGAGTCGATGCACCATGCTCAGTTGACCGaat > 1:1688002/1‑67 (MQ=255)tCGGTACGTCCATAACGACGACGATGTGGACTAACCGCGAGTCGATGCACCATGCTCAGTTGACCGaat > 1:54483/1‑67 (MQ=255)tCGGTACGTCCATAACGACGACGATGTGGACTAACCGCGAGTCGATGCACCATGCTCAGTTGACCGaat > 1:677363/1‑67 (MQ=255)tCGGTACGTCCATAACGACGACGATGTGGACTAACCGCGAGTCGATGCACCATGCTCAGTTGACCGaat > 1:1454930/1‑67 (MQ=255)tCGGTACGTCCATAACGACGACGATGTGGACTAACCGCGAGTCGATGCACCATGCTCAGTTGACCGaat > 1:1412986/1‑67 (MQ=255)tCGGTACGTCCATAACGACGACGATGTGGACTAACCGCGAGTCGATGCACCATGCTCAGTTGACCGaat > 1:1400453/1‑67 (MQ=255)tCGGTACGTCCATAACGACGACGATGTGGACTAACCGCGAGTCGATGCACCATGCTCAGTAGACCGaat > 1:182287/1‑67 (MQ=255)tCGGTACGTCCATAACGACGACGATGTGGACTAACCGCGAGTCGATGCACCATGCTAAGTTGACCGaat > 1:1736555/1‑67 (MQ=255) gATAACCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAAccc > 1:2913360/1‑69 (MQ=255) gATAACCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAAccc > 1:2509535/1‑69 (MQ=255) gATAACCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAAccc > 1:2901747/1‑69 (MQ=255) gATAACCACGACCATGTCGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAAccc > 1:1010062/1‑69 (MQ=255) caTAACGACGACGATGTGGACTAACCGCGAGTCGATGCACCATGCTCAGTTGACCGAATCGGTGAAccc < 1:341012/68‑1 (MQ=255) caTAACGACGACGATGTGGACTAACCGCGAGTCGATGCACCATGCTCAGTTGACCGAATCGGTGAAccc < 1:2080330/68‑1 (MQ=255) caTAACGACGACGATGTGGACTAACCGCGAGTCGATGCACCATGCTCAGTTGACCGAATCGGTGAAccc < 1:315477/68‑1 (MQ=255) caTAACGACGACGATGTGGACTAACCGCGAGTCGATGCACCATGCTCAGTTGACCGAATCGGTGAAccc < 1:3338679/68‑1 (MQ=255) aaCCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTa > 1:2609675/1‑61 (MQ=255) aaCCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAAccc > 1:2764320/1‑66 (MQ=255) aaCCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCg > 1:2480902/1‑67 (MQ=255) aaCCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGt > 1:896833/1‑68 (MQ=255) aaCCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGt > 1:2987470/1‑68 (MQ=255) aaCCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGt > 1:519192/1‑68 (MQ=255) aaCCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGt > 1:766624/1‑68 (MQ=255) aaCCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGt > 1:853984/1‑68 (MQ=255) aaCCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGt > 1:2533764/1‑68 (MQ=255) aaCCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGt > 1:1336392/1‑68 (MQ=255) aaCCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGt > 1:2050279/1‑68 (MQ=255) aaCCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGt > 1:1979293/1‑68 (MQ=255) aaCCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGt > 1:1939249/1‑68 (MQ=255) aaCCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGt > 1:1657737/1‑68 (MQ=255) aaCCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGt > 1:1836883/1‑68 (MQ=255) acaaCGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGTTc > 1:2720635/4‑69 (MQ=255) aCCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGTTc > 1:1234871/1‑69 (MQ=255) aCCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGTTc > 1:1476846/1‑69 (MQ=255) aCCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGTTc > 1:1895334/1‑69 (MQ=255) cgAGTGGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGTTCAACCCGAATGCCCAGGCGATGTa < 1:396752/69‑1 (MQ=255) cgAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGTTCAACCCGAATGCCCAGGCGATGTa < 1:120036/69‑1 (MQ=255) cgAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGTTCAACCCGAATGCCCAGGCGATGTa < 1:1521472/69‑1 (MQ=255) cgAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGTTCAACCCGAATGCCCAGGCGATGTa < 1:1785210/69‑1 (MQ=255) cgAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGTTCAACCCGAATGCCCAGGCGATGTa < 1:3205706/69‑1 (MQ=255) cgAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGTTCAACCCGAATGCCCAGGCGATGTa < 1:2111912/69‑1 (MQ=255) cgcAGTTGACTGAGTCGGTAAACCCGTTCAACCCGAATGCCCAGGCGATGTACAGTCAACTGGAAGGGc > 1:246213/1‑69 (MQ=255) cgcAGTTGACTGAGTCGGTAAACCCGTTCAACCCGAATGCCCAGGCGATGTACAGTCAACTGGAAGGGc > 1:2739576/1‑69 (MQ=255) gTTGACTGAGTCGGTAAACCCGTTCAACCCGAATGCCCAGGCGATGTACAGTCAACTGGAAGGGCTTgg > 1:3190420/1‑69 (MQ=255) gTTGACTGAGTCGGTAAACCCGTTCAACCCGAATGCCCAGGCGATGTACAGTCAACTGGAAGGGCTTgg > 1:693489/1‑69 (MQ=255) ttGACTGAGTCGGTAAACCCGTTCAACCCGAATGCCCAGGCGATGTACAGTCAACTGGAAGGGCTTggg > 1:1917362/1‑69 (MQ=255) ttGACTGAGTCGGTAAACCCGTTCAACCCGAATGCCCAGGCGATGTACAGTCAACTGGAAGGGCTTggg > 1:3072449/1‑69 (MQ=255) | TCGGCACGTCGATAACCACGACCATGTGGACCAACCGCGAGTCGATGCACCATGCGCAGTTGACTGAGTCGGTAAACCCGTTCAACCCGAATGCCCAGGCGATGTACAGTCAACTGGAAGGGCTTGGG > minE/1807015‑1807142 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |