Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,820,412 T→C 35.7% L32L (CTA→CTG)  norR ← DNA‑binding transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,820,4120TC35.7% 78.8 / 75.9 123L32L (CTA→CTG) norRDNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (35/44);  new base C (31/13);  total (66/57)
Fisher's exact test for biased strand distribution p-value = 7.96e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

GCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCAATCCCACGCTGCAATTC  >  minE/1820344‑1820468
                                                                    |                                                        
gCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGt                                                          <  1:1911456/69‑1 (MQ=255)
gCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGt                                                          <  1:3273442/69‑1 (MQ=255)
gCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGt                                                          <  1:2386106/69‑1 (MQ=255)
 cAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGt                                                          <  1:1787371/68‑1 (MQ=255)
  aaGCGGAATAAAGTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAg                                                        <  1:95006/69‑1 (MQ=255)
  aaGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAg                                                        <  1:3367918/69‑1 (MQ=255)
  aaGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAg                                                        <  1:231457/69‑1 (MQ=255)
  aaGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAg                                                        <  1:2185734/69‑1 (MQ=255)
  aaGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAg                                                        <  1:69915/69‑1 (MQ=255)
  aaGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAg                                                        <  1:2553956/69‑1 (MQ=255)
  aaGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAg                                                        <  1:3090543/69‑1 (MQ=255)
  aaGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAg                                                        <  1:1289356/69‑1 (MQ=255)
  aaGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAg                                                        <  1:2665974/69‑1 (MQ=255)
  aaGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAg                                                        <  1:2723328/69‑1 (MQ=255)
  aaGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAg                                                        <  1:2756085/69‑1 (MQ=255)
  aaGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAg                                                        <  1:2133374/69‑1 (MQ=255)
  aaGCGGAATAAACTGCCCCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAg                                                        <  1:1031304/69‑1 (MQ=255)
      ggAATAAACTGCCGCGAGTCTTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGCAGCgtg                                                    <  1:3243840/69‑1 (MQ=255)
      ggAATAAACTGCCGCGAGTCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGCAGCgtg                                                    <  1:1558776/69‑1 (MQ=255)
      ggAATAAACTGCCGCGAGTCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGCAGCgtg                                                    <  1:3348043/69‑1 (MQ=255)
      ggAATAAACTGCCGCGAGTCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGCAGCgtg                                                    <  1:3132397/69‑1 (MQ=255)
      ggAATAAACTGCCGCGAGTCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGCAGCgtg                                                    <  1:424184/69‑1 (MQ=255)
      ggAATAAACTGCCGCGAGTCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGCAGCgtg                                                    <  1:2142236/69‑1 (MQ=255)
      ggAATAAACTGCCGCGAGTCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGCAGCgtg                                                    <  1:2949571/69‑1 (MQ=255)
      ggAATAAACTGCCGCGAGTCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGCAGCgtg                                                    <  1:611817/69‑1 (MQ=255)
      ggAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtg                                                    <  1:2141310/69‑1 (MQ=255)
      ggAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtg                                                    <  1:2994015/69‑1 (MQ=255)
      ggAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtg                                                    <  1:2915529/69‑1 (MQ=255)
      ggAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtg                                                    <  1:2102567/69‑1 (MQ=255)
      ggAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtg                                                    <  1:502561/69‑1 (MQ=255)
      ggAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtg                                                    <  1:127029/69‑1 (MQ=255)
      ggAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtg                                                    <  1:174466/69‑1 (MQ=255)
      ggAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtg                                                    <  1:1251673/69‑1 (MQ=255)
      ggAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtg                                                    <  1:1071466/69‑1 (MQ=255)
      ggAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtg                                                    <  1:1680533/69‑1 (MQ=255)
      ggAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGAACTCCAGCACCTGACGTAGCgtg                                                    <  1:1675325/69‑1 (MQ=255)
      gcaaTAAACTGCCGCGAGTCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACCCAGCgtg                                                    <  1:3021423/67‑1 (MQ=255)
       gAATAAACTGCCGCGAGTCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGCAGCgtg                                                    <  1:1417049/68‑1 (MQ=255)
          tAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtggtg                                                 >  1:1730586/1‑68 (MQ=255)
          tAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtggtg                                                 >  1:1106134/1‑68 (MQ=255)
          tAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtggtg                                                 >  1:2323564/1‑68 (MQ=255)
          tAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtggtg                                                 >  1:70914/1‑68 (MQ=255)
          tAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtggtg                                                 >  1:2920719/1‑68 (MQ=255)
          tAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtggtg                                                 >  1:1422281/1‑68 (MQ=255)
          tAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtggtg                                                 >  1:1369996/1‑68 (MQ=255)
          tAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtggtg                                                 >  1:2592570/1‑68 (MQ=255)
                      aaTCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGaa                                    <  1:2453736/69‑1 (MQ=255)
                      aaTCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGaa                                    <  1:2932941/69‑1 (MQ=255)
                      aaTCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGaa                                    <  1:2656949/69‑1 (MQ=255)
                      aaTCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGaa                                    <  1:208913/69‑1 (MQ=255)
                      aaTCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGaa                                    <  1:1521592/69‑1 (MQ=255)
                      aaTCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGaa                                    <  1:795709/69‑1 (MQ=255)
                      aaTCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGaa                                    <  1:3403170/69‑1 (MQ=255)
                      aaTCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGaa                                    <  1:694084/69‑1 (MQ=255)
                                        cAGACGCATCGCACTCCAGCACCTGAGGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTg                          >  1:2773258/1‑61 (MQ=255)
                                        cAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCaa                  >  1:1395009/1‑69 (MQ=255)
                                        cAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCaa                  >  1:2611070/1‑69 (MQ=255)
                                        cAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCaa                  >  1:880167/1‑69 (MQ=255)
                                        cAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCaa                  >  1:954156/1‑69 (MQ=255)
                                        cAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCaa                  >  1:3001195/1‑69 (MQ=255)
                                        cAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCaa                  >  1:3416984/1‑69 (MQ=255)
                                        cAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCaa                  >  1:2144328/1‑69 (MQ=255)
                                        cAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCaa                  >  1:634509/1‑69 (MQ=255)
                                                       ccAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCAATCCCACGCTGCAAt    >  1:1066624/1‑68 (MQ=255)
                                                       ccAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCAATCCCACGCTGCAAt    >  1:2893300/1‑68 (MQ=255)
                                                       ccAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCAATCCCACGCTGCAAt    >  1:2897406/1‑68 (MQ=255)
                                                        cAGCACCTGACGTAGCGTGGTGATCAGGCGCTGaa                                    >  1:1879316/1‑35 (MQ=255)
                                                        cAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGa                               >  1:1916114/1‑40 (MQ=255)
                                                        cAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCAATCCCACGCTGCAATTc  >  1:958864/1‑69 (MQ=255)
                                                        cAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCAATCCCACGCTGCAATTc  >  1:1156968/1‑69 (MQ=255)
                                                        cAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCAATCCCACGCTGCAATTc  >  1:2232236/1‑69 (MQ=255)
                                                        cAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCAATCCCACGCTGCAATTc  >  1:2647714/1‑69 (MQ=255)
                                                        cAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCAATCCCACGCTGCAATTc  >  1:2349047/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATc                >  1:1738931/1‑55 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAAtt   >  1:3311368/1‑68 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:2145020/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:2205580/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:1909721/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:622273/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:1710566/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:1545762/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:1540529/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:807118/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:1409319/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:1383008/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:403516/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:364901/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:2343767/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:3312472/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:2369610/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:24278/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:251773/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:3032900/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:2762687/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:2792868/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:2869670/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:2981641/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:2926308/1‑69 (MQ=255)
                                                        cAGCACCTGACGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAACCCCACGCTGCAATTc  >  1:287588/1‑69 (MQ=255)
                                                        cAGCACCTGAAGCAGCGTGGTGATCAGGCGTTGAAAACGATCCTGATGACCAATCCCACGCTGCAATTc  >  1:893622/1‑69 (MQ=255)
                                                                    |                                                        
GCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGTGATCAGGCGCTGAAAACGATCCTGGTGACCAATCCCACGCTGCAATTC  >  minE/1820344‑1820468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑