| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,820,972 | A→G | 14.0% | A93A (GCA→GCG) | norV → | flavorubredoxin oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,820,972 | 0 | A | G | 14.0% | 160.7 / 12.5 | 92 | A93A (GCA→GCG) | norV | flavorubredoxin oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (50/29); new base G (7/6); total (57/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
TATCGATTACATCGTGATTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCGATAAATG > minE/1820906‑1821027 | tatCGATTACATCGTGATTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGcaca < 1:1080810/69‑1 (MQ=255)tatCGATTACATCGTGATTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGcaca < 1:277184/69‑1 (MQ=255)tatCGATTACATCGTGATTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGcaca < 1:2674512/69‑1 (MQ=255)tatCGATTACATCGTGATTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGcaca < 1:2214527/69‑1 (MQ=255) atCGATTACATCGTGATTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGcaca < 1:1925645/68‑1 (MQ=255) atCGATTACATCGTGATTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGcaca < 1:1915022/68‑1 (MQ=255) cGATTACATCGTGATTAACCATGCAGAAGAGGACCACGCTGTGGCGCTGACCGAACTGATGGCACAAAt > 1:1186340/1‑69 (MQ=255) cGATTACATCGTGATTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAAt > 1:2077958/1‑69 (MQ=255) cGATTACATCGTGATTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAAt > 1:1768653/1‑69 (MQ=255) gATTACATCGTGATTACCCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAAtt > 1:532299/1‑69 (MQ=255) gATTACATCGTGATTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAAtt > 1:1959833/1‑69 (MQ=255) gATTACATCGTGATTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAAtt > 1:1892150/1‑69 (MQ=255) gATTACATCGTGATTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAAtt > 1:3034729/1‑69 (MQ=255) tACATCGTGATTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTccc > 1:3343386/1‑69 (MQ=255) tACATCGTGATTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTccc > 1:666652/1‑69 (MQ=255) aTCGTGATTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGa < 1:940991/68‑1 (MQ=255) aTTAACCATGCTGAAGAGGACCACGCCGGGGCGCTGACCGAACTGATGGCGCAAATTCCCGATACGcc > 1:636028/1‑68 (MQ=255) aTTAACCATGCTGAAGAGGACCACGCCGGGGCGCTGACCGAACTGATGGCGCAAATTCCCGATACGCCg > 1:2388422/1‑69 (MQ=255) aTTAACCATGCTGAAGAGGACCACGCCGGGGCGCTGACCGAACTGATGGCGCAAATTCCCGATACGCCg > 1:1607372/1‑69 (MQ=255) aTTAACCATGCTGAAGAGGACCACGCCGGGGCGCTGACCGAACTGATGGCGCAAATTCCCGATACGCCg > 1:3100442/1‑69 (MQ=255) aTTAACCATGCTGAAGAGGACCACGCCGGGGCGCTGACCGAACTGATGGCGCAAATTCCCGATACGCCg > 1:2819752/1‑69 (MQ=255) aTTAACCATGCTGAAGAGGACCACGCCGGGGCGCTGACCGAACTGATGGCGCAAATTCCCGATACGCCg > 1:3234135/1‑69 (MQ=255) aTTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCg < 1:2235644/69‑1 (MQ=255) aTTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCg < 1:595418/69‑1 (MQ=255) aTTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCg < 1:1332025/69‑1 (MQ=255) aTTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCg < 1:1359635/69‑1 (MQ=255) ttAACCATGCTGAAGAGGACCACGCCGGGGCGCTGACCGAACTGATGGCGCAAATTCCCGATACGCCGa > 1:489294/1‑69 (MQ=255) tAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATa < 1:612273/62‑1 (MQ=255) tAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATa < 1:2471268/62‑1 (MQ=255) tAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATa > 1:2707402/1‑62 (MQ=255) tAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATa < 1:2269968/62‑1 (MQ=255) tAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATa < 1:3286399/62‑1 (MQ=255) tAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATa > 1:1964582/1‑62 (MQ=255) tAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATa < 1:3351666/62‑1 (MQ=255) tAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATa > 1:1762628/1‑62 (MQ=255) tAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATa > 1:72360/1‑62 (MQ=255) tAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATa < 1:931412/62‑1 (MQ=255) tAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATa > 1:949515/1‑62 (MQ=255) tAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATa > 1:1382730/1‑62 (MQ=255) tAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATa > 1:1373531/1‑62 (MQ=255) tAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATa > 1:324613/1‑62 (MQ=255) tAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATa < 1:3279601/62‑1 (MQ=255) tAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATa > 1:1179745/1‑62 (MQ=255) cAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCg < 1:2604154/59‑1 (MQ=255) cAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTg > 1:451224/1‑67 (MQ=255) cAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTg > 1:3302793/1‑67 (MQ=255) cAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTg > 1:46890/1‑67 (MQ=255) agagGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCg > 1:1136855/1‑47 (MQ=255) agagGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTg > 1:1187129/1‑63 (MQ=255) agagGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTg > 1:626641/1‑63 (MQ=255) agagGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTg > 1:1114818/1‑63 (MQ=255) agagGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTg > 1:797001/1‑63 (MQ=255) agagGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTg > 1:2845913/1‑63 (MQ=255) agagGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTg > 1:1785983/1‑63 (MQ=255) agagGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTg > 1:1880233/1‑63 (MQ=255) agagGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTg > 1:2966777/1‑63 (MQ=255) agagGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCAGATACGCCGATCTACt > 1:2262643/1‑62 (MQ=255) gACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGcc > 1:1016753/1‑66 (MQ=255) gACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAAc > 1:2874267/1‑69 (MQ=255) gACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAAc > 1:1648730/1‑69 (MQ=255) gACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAAc > 1:3356933/1‑69 (MQ=255) gACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAAc > 1:1923783/1‑69 (MQ=255) gACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAAc > 1:2753869/1‑69 (MQ=255) ccggggCGCTGACCGAACTGATGGCGCAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCg < 1:2900319/67‑1 (MQ=255) ccggggCGCTGACCGAACTGATGGCGCAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCg < 1:1304778/67‑1 (MQ=255) ccggggCGCTGACCGAACTGATGGCGCAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCg < 1:952931/67‑1 (MQ=255) ccggggCGATGACCGAACTGATGGCGCAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCg < 1:2495246/67‑1 (MQ=255) tGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGa < 1:880709/69‑1 (MQ=255) tGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGa < 1:587673/69‑1 (MQ=255) tGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGa < 1:1154841/69‑1 (MQ=255) tGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGa < 1:1376270/69‑1 (MQ=255) tGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGa < 1:3017027/69‑1 (MQ=255) tGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGa < 1:1407157/69‑1 (MQ=255) tGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGa < 1:1479976/69‑1 (MQ=255) tGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGa < 1:1608049/69‑1 (MQ=255) tGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGa < 1:2637200/69‑1 (MQ=255) cggggCGCTGACCGAACTGATGGCGCAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCg < 1:2581355/67‑1 (MQ=255) ggggCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGa < 1:258211/68‑1 (MQ=255) cgcTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTc > 1:210680/1‑67 (MQ=255) cgcTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCg > 1:2393920/1‑68 (MQ=255) cgcTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCg > 1:445810/1‑68 (MQ=255) cgcTGACCGAACTGATGGCACAAATTCCAGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCg > 1:3096067/1‑68 (MQ=255) cTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCGATa < 1:1708988/69‑1 (MQ=255) cTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCGATa > 1:1683861/1‑69 (MQ=255) cTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCGATa < 1:1549375/69‑1 (MQ=255) cTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCGATa > 1:948575/1‑69 (MQ=255) cTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCGATa < 1:3047041/69‑1 (MQ=255) cTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCGATa > 1:2272683/1‑69 (MQ=255) cTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCGATa > 1:241078/1‑69 (MQ=255) cTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCGATa < 1:1099639/69‑1 (MQ=255) cTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCGATa > 1:484597/1‑69 (MQ=255) cTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCGATa > 1:270272/1‑69 (MQ=255) cTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCGATa > 1:2796662/1‑69 (MQ=255) cTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCGATa < 1:412551/69‑1 (MQ=255) cTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCGATa < 1:392508/69‑1 (MQ=255) cTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCGATa > 1:3044390/1‑69 (MQ=255) tGACCGAACTGATGGCGCAAATTCCCGATACGCCGATCTACTg < 1:3414057/43‑1 (MQ=255) gATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCGATAAATg > 1:961784/1‑62 (MQ=255) | TATCGATTACATCGTGATTAACCATGCAGAAGAGGACCACGCTGGGGCGCTGACCGAACTGATGGCACAAATTCCCGATACGCCGATCTACTGTACAGCCAACGCTATCGACTCGATAAATG > minE/1820906‑1821027 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |