| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,826,057 | G→A | 40.4% | C89C (TGC→TGT) | hydN ← | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,826,057 | 0 | G | A | 40.4% | 42.9 / 64.7 | 94 | C89C (TGC→TGT) | hydN | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Reads supporting (aligned to +/- strand): ref base G (8/48); new base A (30/8); total (38/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.04e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.70e-01 | |||||||||||
GGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCG > minE/1825995‑1826112 | ggACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGt < 1:2382082/69‑1 (MQ=255)ggACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGt < 1:632330/69‑1 (MQ=255)ggACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGt < 1:1977568/69‑1 (MQ=255) gcaccaccACTTCCATCGCACCATACGGGCAAGCCACAACGCAGG‑TTTGCAACCAATACAACGTTCCTg < 1:1109903/67‑1 (MQ=255) caccaccACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTg < 1:1839089/68‑1 (MQ=255) caccaccACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTg < 1:1941835/68‑1 (MQ=255) caccaccACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTg < 1:2751091/68‑1 (MQ=255) caccaccACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTg < 1:3131434/68‑1 (MQ=255) caccaccACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTg < 1:882258/68‑1 (MQ=255) accaccACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTg < 1:1059809/68‑1 (MQ=255) accaccACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTg < 1:3296520/68‑1 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCATGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:2559448/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTTCAtga > 1:230923/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:2799605/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:2702831/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:190024/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:3274109/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:3289169/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:2365953/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:2543939/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:3360680/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:3360794/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:3382175/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:3386123/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:1131313/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:898990/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:1214330/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:889124/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:970680/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:1375421/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:1424302/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:1443962/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:788914/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:633181/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:1576877/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:450933/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:1055175/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtga > 1:1667714/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtg > 1:1877275/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtg > 1:134386/1‑67 (MQ=255) ccacTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATACAACGTTCCTGCAtg > 1:1185998/1‑67 (MQ=255) tCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACaa < 1:390899/69‑1 (MQ=255) gCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAc < 1:2605121/69‑1 (MQ=255) gCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAc < 1:299686/69‑1 (MQ=255) gCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAc < 1:446504/69‑1 (MQ=255) gCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAc < 1:975914/69‑1 (MQ=255) gCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAc > 1:1065827/1‑69 (MQ=255) gCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAc < 1:1721303/69‑1 (MQ=255) aCCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAccc < 1:2778122/69‑1 (MQ=255) aCCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAccc < 1:3246611/69‑1 (MQ=255) aCCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAccc < 1:1817931/69‑1 (MQ=255) aCCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAccc < 1:1757459/69‑1 (MQ=255) aCCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAccc < 1:300272/69‑1 (MQ=255) aCCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAccc < 1:2049547/69‑1 (MQ=255) aCCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAccc < 1:16251/69‑1 (MQ=255) aCCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAccc < 1:2117138/69‑1 (MQ=255) aCCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAccc < 1:835651/69‑1 (MQ=255) aCCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAccc < 1:2641200/69‑1 (MQ=255) aCCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAccc < 1:2477304/69‑1 (MQ=255) aCCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAAccc < 1:2527369/69‑1 (MQ=255) cGGGCAAGCCACAACGCAGGTTTTGCAACCAATGc > 1:2554371/1‑35 (MQ=255) cGGGCAAGCCACAACGCAGGTTTTGCAACCAATGc > 1:456629/1‑35 (MQ=255) cGGGCAAGCCACAACGCAGGTTTTGCAACCAATGc > 1:1481610/1‑35 (MQ=255) cGGGCAAGCCACAACGCAGGTTTTGCAACCAATGc > 1:1286755/1‑35 (MQ=255) cGGGCAAGCCACAACGCAGGTTTTGCAACCAATGc > 1:3017850/1‑35 (MQ=255) cGGGCAAGCCACAACGCAGGTTTTGCAACCAATGc > 1:2961787/1‑35 (MQ=255) ggCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCAc < 1:3263127/69‑1 (MQ=255) ggCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCAc < 1:1212950/69‑1 (MQ=255) ggCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCAc < 1:1814438/69‑1 (MQ=255) ggCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCAc < 1:2035715/69‑1 (MQ=255) ggCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCAc < 1:2206176/69‑1 (MQ=255) ggCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCAc < 1:2711276/69‑1 (MQ=255) gCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACg < 1:1898404/69‑1 (MQ=255) gCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACg < 1:905467/69‑1 (MQ=255) gCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACg < 1:1861107/69‑1 (MQ=255) gCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACg < 1:857251/69‑1 (MQ=255) gCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACg < 1:1743820/69‑1 (MQ=255) gCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACg < 1:2062045/69‑1 (MQ=255) gCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACg < 1:1567527/69‑1 (MQ=255) aGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACg < 1:1060631/66‑1 (MQ=255) aGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCt < 1:460535/69‑1 (MQ=255) aGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCt < 1:1860925/69‑1 (MQ=255) gCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCt < 1:3208499/68‑1 (MQ=255) ttttGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCg < 1:1497202/69‑1 (MQ=255) ttttGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCg < 1:1649673/69‑1 (MQ=255) ttttGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCg < 1:79053/69‑1 (MQ=255) ttttGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCg < 1:3172412/69‑1 (MQ=255) ttttGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCg < 1:269936/69‑1 (MQ=255) ttttGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCg < 1:3280406/69‑1 (MQ=255) ttttGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCg < 1:921795/69‑1 (MQ=255) tttGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACg < 1:836063/51‑1 (MQ=255) tttGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGa < 1:358941/56‑1 (MQ=255) tttGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGa < 1:2284376/56‑1 (MQ=255) tttGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTc > 1:1253373/1‑67 (MQ=255) | GGACGTACCACCACTTCCATCGCACCATACGGGCAAGCCACAACGCAGGTTTTGCAACCAATGCAACGTTCCTGCATCACATGAACAAACCCTTTATCACGGCTGATAGCACCATTCG > minE/1825995‑1826112 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |