| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,826,895 | C→G | 63.4% | R46R (CGC→CGG) | mutS → | methyl‑directed mismatch repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,826,895 | 0 | C | G | 63.4% | 45.2 / 49.4 | 60 | R46R (CGC→CGG) | mutS | methyl‑directed mismatch repair protein |
| Reads supporting (aligned to +/- strand): ref base C (17/5); new base G (24/14); total (41/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCG > minE/1826833‑1826952 | gagaTCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTc > 1:2963186/1‑68 (MQ=255) gctgTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAActgc > 1:3186286/1‑68 (MQ=255) gctgTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAActgc > 1:2164316/1‑68 (MQ=255) gTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTgg > 1:2716797/1‑68 (MQ=255) gTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGa > 1:1261863/1‑69 (MQ=255) gTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGa < 1:256605/69‑1 (MQ=255) gTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGa < 1:2443226/69‑1 (MQ=255) gTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGa > 1:1768370/1‑69 (MQ=255) ttACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGatat > 1:1369490/1‑69 (MQ=255) ttACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGata > 1:1827914/1‑68 (MQ=255) ttACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGa < 1:749723/66‑1 (MQ=255) ggTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGAc > 1:2950671/1‑68 (MQ=255) ttttATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAAcgc > 1:3103677/1‑69 (MQ=255) ttttATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAAcgc > 1:502230/1‑69 (MQ=255) ttttATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAAcgc > 1:2608307/1‑69 (MQ=255) ttttATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAAcgc > 1:497762/1‑69 (MQ=255) ttttATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAAcgc > 1:465829/1‑69 (MQ=255) ttttATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAAcgc > 1:3212183/1‑69 (MQ=255) ttttATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAAcgc > 1:1546343/1‑69 (MQ=255) ttttATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAAcgc > 1:1799245/1‑69 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAAcg > 1:1590058/1‑59 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:3155659/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:2981389/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:393185/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:371913/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:3295606/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:351999/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:3126249/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:3304938/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:26868/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:2578039/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:597146/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:1972785/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:6910/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:1793313/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:77683/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:1429877/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:1407465/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:1406901/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:1284208/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:1174529/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTc < 1:571037/68‑1 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTc < 1:582960/68‑1 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTc < 1:630676/68‑1 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTc < 1:790233/68‑1 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTc < 1:337197/68‑1 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTc < 1:2991024/68‑1 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTc < 1:2888486/68‑1 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTc < 1:2813674/68‑1 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTc < 1:2788582/68‑1 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTc < 1:2770572/68‑1 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTc > 1:2242917/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTc < 1:1952447/68‑1 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTc < 1:1885065/68‑1 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTc < 1:1645150/68‑1 (MQ=255) cTGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACAAAACGCGGTGCTTca > 1:2463666/1‑68 (MQ=255) cTGTTTTATGACGACGCAAAACGGGAGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTca > 1:40441/1‑68 (MQ=255) tGTTTTATGACGACGCAAAACGGGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTc < 1:238726/67‑1 (MQ=255) gacgCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCg < 1:1800260/69‑1 (MQ=255) gacgCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCg < 1:1090975/69‑1 (MQ=255) | GAGATCCTGCTGTTTTACCGGATGGGTGATTTTTATGAACTGTTTTATGACGACGCAAAACGCGCGTCGCAACTGCTGGATATTTCACTGACCAAACGCGGTGCTTCGGCGGGAGAGCCG > minE/1826833‑1826952 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |