| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,827,216 | G→T | 67.8% | G153G (GGG→GGT) | mutS → | methyl‑directed mismatch repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,827,216 | 0 | G | T | 67.8% | 63.2 / 60.1 | 81 | G153G (GGG→GGT) | mutS | methyl‑directed mismatch repair protein |
| Reads supporting (aligned to +/- strand): ref base G (17/9); new base T (18/37); total (35/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.09e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-01 | |||||||||||
TGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATC > minE/1827152‑1827280 | tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:1373527/69‑1 (MQ=255)tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:2326274/69‑1 (MQ=255)tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:1974369/69‑1 (MQ=255)tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:1851477/69‑1 (MQ=255)tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:2693474/69‑1 (MQ=255)tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:2724242/69‑1 (MQ=255)tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:2922468/69‑1 (MQ=255)tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:3098516/69‑1 (MQ=255)tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:1580649/69‑1 (MQ=255)tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:3427345/69‑1 (MQ=255)tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:1252291/69‑1 (MQ=255)tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:1000161/69‑1 (MQ=255)tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:1073089/69‑1 (MQ=255)tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:966504/69‑1 (MQ=255)tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:116890/69‑1 (MQ=255)tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:919721/69‑1 (MQ=255)tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:775961/69‑1 (MQ=255)tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:1268693/69‑1 (MQ=255)tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:1272456/69‑1 (MQ=255)tgctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:878201/69‑1 (MQ=255) gctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:1780706/68‑1 (MQ=255) gctgGCGGCTATCTGGCAGGACAGTAAAGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:2673495/68‑1 (MQ=255) tgtcAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGaa > 1:1621223/4‑69 (MQ=255) tGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGaa > 1:3307423/1‑69 (MQ=255) tGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGaa > 1:1024623/1‑69 (MQ=255) tGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGaa > 1:2598105/1‑69 (MQ=255) tGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGaa > 1:419198/1‑69 (MQ=255) tGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGaa > 1:1693313/1‑69 (MQ=255) aCAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTg > 1:2267202/1‑69 (MQ=255) aCAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTg > 1:132312/1‑69 (MQ=255) aCAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTg > 1:192207/1‑69 (MQ=255) aaaGGTTTCGGCTACGCAACGCTGGATATCAGTTCCGGTCGtt < 1:3058490/43‑1 (MQ=38) ggCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGAt < 1:2056583/68‑1 (MQ=255) ggCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGAt < 1:2093320/68‑1 (MQ=255) ggCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGAt < 1:128779/68‑1 (MQ=255) ggCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGAt < 1:2219390/68‑1 (MQ=255) tACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATggc > 1:16355/1‑68 (MQ=255) tACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATggc > 1:2706250/1‑68 (MQ=255) tACGCGACGCTGCATATCAGTTCCGGGCGTTTTCCCCTGAGCGAAc > 1:2614531/1‑46 (MQ=39) cgACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAg < 1:2022611/69‑1 (MQ=255) cgACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAg < 1:2764869/69‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGCGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:1318287/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGCACCGGCGGACCGCGaa > 1:429991/2‑55 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGGGAAACGATGGCGGCAGa > 1:3154256/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAg > 1:840002/2‑68 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:910358/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:1625169/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:1579637/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:3324490/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:1197305/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:3359621/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:3397973/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:826976/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:618943/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:1102612/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:808233/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:75983/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:2647188/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:2115066/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:2159713/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:2174639/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:2313989/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:252880/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:2585861/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:308852/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:1728403/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:2700958/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:2739237/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:3018624/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:3033091/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCATGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:3009112/2‑69 (MQ=255) aaCGCTGGATATCACTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:3267556/68‑1 (MQ=255) aaCGCTCGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGaaa > 1:438637/2‑56 (MQ=255) cTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACt > 1:1370106/1‑68 (MQ=255) cTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACt > 1:2312400/1‑68 (MQ=255) cTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACt > 1:2164915/1‑68 (MQ=255) cTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACt > 1:930819/1‑68 (MQ=255) ttCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTaa < 1:412604/69‑1 (MQ=255) ttCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTaa < 1:1908359/69‑1 (MQ=255) ttCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTaa < 1:658397/69‑1 (MQ=255) ccGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATc > 1:1991866/1‑69 (MQ=255) | TGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATC > minE/1827152‑1827280 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |