| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,827,240 | T→G | 55.2% | A161A (GCT→GCG) | mutS → | methyl‑directed mismatch repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,827,240 | 0 | T | G | 55.2% | 21.5 / 54.9 | 58 | A161A (GCT→GCG) | mutS | methyl‑directed mismatch repair protein |
| Reads supporting (aligned to +/- strand): ref base T (13/13); new base G (18/14); total (31/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
ACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTA > minE/1827173‑1827296 | aCAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTg > 1:132312/1‑69 (MQ=255)aCAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTg > 1:2267202/1‑69 (MQ=255)aCAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTg > 1:192207/1‑69 (MQ=255) ggCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGAt < 1:128779/68‑1 (MQ=255) ggCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGAt < 1:2093320/68‑1 (MQ=255) ggCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGAt < 1:2056583/68‑1 (MQ=255) ggCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGAt < 1:2219390/68‑1 (MQ=255) tACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATggc > 1:2706250/1‑68 (MQ=255) tACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATggc > 1:16355/1‑68 (MQ=255) cgACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAg < 1:2764869/69‑1 (MQ=255) cgACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAg < 1:2022611/69‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGCGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:1318287/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGCACCGGCGGACCGCGaa > 1:429991/2‑55 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGGGAAACGATGGCGGCAGa > 1:3154256/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAg > 1:840002/2‑68 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:2739237/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:2700958/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:2647188/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:308852/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:826976/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:3018624/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:3033091/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:2313989/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:3324490/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:3359621/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:3397973/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:618943/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:75983/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:808233/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:252880/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:1197305/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:1102612/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:1579637/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:1625169/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:1728403/68‑1 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:2115066/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:2159713/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:2174639/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:910358/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:2585861/2‑69 (MQ=255) aaCGCTGGATATCAGTTCCGGTCGTTTTCGCATGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa > 1:3009112/2‑69 (MQ=255) aaCGCTGGATATCACTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGAAACGATGGCGGCAGa < 1:3267556/68‑1 (MQ=255) aaCGCTCGATATCAGTTCCGGTCGTTTTCGCCTGAGCGAACCGGCGGACCGCGaaa > 1:438637/2‑56 (MQ=255) cTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACt > 1:2164915/1‑68 (MQ=255) cTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACt > 1:930819/1‑68 (MQ=255) cTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACt > 1:2312400/1‑68 (MQ=255) cTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACt > 1:1370106/1‑68 (MQ=255) ttCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTaa < 1:1908359/69‑1 (MQ=255) ttCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTaa < 1:412604/69‑1 (MQ=255) ttCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTaa < 1:658397/69‑1 (MQ=255) ccGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATc > 1:1991866/1‑69 (MQ=255) cGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGc > 1:625788/1‑68 (MQ=255) ttCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAAc < 1:98919/69‑1 (MQ=255) ttCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAAc < 1:2587818/69‑1 (MQ=255) ttCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAAc < 1:2254780/69‑1 (MQ=255) ttCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAAc > 1:1347957/1‑69 (MQ=255) aGCGAACCGGCTGACCGCGAAACGATGGCGCCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTa < 1:294703/68‑1 (MQ=255) gCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGt > 1:1796693/1‑66 (MQ=255) | ACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTA > minE/1827173‑1827296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |