| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,829,862 | T→G | 35.8% | S146R (AGT→AGG) ‡ | pphB → | serine/threonine‑specific protein phosphatase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,829,862 | 0 | T | G | 35.8% | 32.4 / 42.3 | 53 | S146R (AGT→AGG) ‡ | pphB | serine/threonine‑specific protein phosphatase 2 |
| Reads supporting (aligned to +/- strand): ref base T (18/16); new base G (14/5); total (32/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
ATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAA > minE/1829798‑1829927 | atatTATTGAAATCACTAACGACACCATAAAATATGTCATCGCGCATGCAGATTATCCGGGGAAGGAat > 1:1131611/1‑69 (MQ=255)atatTATTGAAATCACTAACGACACCATAAAATATGTCATCGCACATGCAGATTATCCGGGGAAGGAat > 1:2569050/1‑69 (MQ=255)atatTATTGAAATCACTAACGACACCATAAAATATGTCATCGCACATGCAGATTATCCGGGGAAGGAat > 1:2642739/1‑69 (MQ=255)atatTATTGAAATCACTAACGACACCATAAAATATGTCATCGCACATGCAGATTATCCGGGGAAGGAat > 1:492035/1‑69 (MQ=255)atatTATTGAAATCACTAACGACACCATAAAATATGTCATCGCACATGCAGATTATCCGGGGAAGGAat > 1:455349/1‑69 (MQ=255)atatTATTGAAATCACTAACGACACCATAAAATATGTCATCGCACATGCAGATTATCCGGGGAAGGAat > 1:38391/1‑69 (MQ=255)atatTATTGAAATCACTAACGACACCATAAAATATGTCATCGCACATGCAGATTATCCGGGGAAGGAat > 1:2889281/1‑69 (MQ=255)atatTATTGAAATCACTAACGACACCATAAAATATGTCATCGCACATGCAGATTATCCGGGGAAGGAat > 1:3007294/1‑69 (MQ=255)atatTATTGAAATCACTAACGACACCATAAAATATGTCATCGCACATGCAGATTATCCGGGGAAGGAat > 1:3078514/1‑69 (MQ=255) aTCACTAACGACAACATAAAATATGCCATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTgg > 1:1689629/1‑68 (MQ=255) aTCACTAACGACAACATAAAATATGCCATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTgg > 1:2535826/1‑68 (MQ=255) aTCACTAACGACAACATAAAATATGCCATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTgg > 1:2730288/1‑68 (MQ=255) aTCACTAACGACAACATAAAATATGCCATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTgg > 1:2863092/1‑68 (MQ=255) aTCACTAACGACAACATAAAATATGCCATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTgg > 1:3181492/1‑68 (MQ=255) aTCACTAACGACAACATAAAATATGCCATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTgg > 1:3354747/1‑68 (MQ=255) aTCACTAACGACAACATAAAATATGCCATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTgg > 1:1210369/1‑68 (MQ=255) aTCACTAACGACAACATAAAATATGCCATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTgg > 1:1000253/1‑68 (MQ=255) aTCACTAACGACAACATAAAATATGCCATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTg > 1:2169174/1‑67 (MQ=255) cACTAACGACAACATAAAATATGCCATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTgg > 1:3162262/1‑66 (MQ=255) cACTAACGACAACATAAAATATGCCATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTgg > 1:3059921/1‑66 (MQ=255) cACTAACGACAACATAAAATATGCCATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTgg > 1:2485230/1‑66 (MQ=255) tAACGACAACATAAAATATGCCATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGa < 1:1245858/69‑1 (MQ=255) tAACGACAACATAAAATATGCCATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGa < 1:61972/69‑1 (MQ=255) tAACGACAACATAAAATATGCCATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGa < 1:1096971/69‑1 (MQ=255) ccATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTa < 1:1024594/68‑1 (MQ=255) ccATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTa < 1:1949555/68‑1 (MQ=255) ccATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTa < 1:1945561/68‑1 (MQ=255) ccATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTa < 1:1873970/68‑1 (MQ=255) ccATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTa < 1:1844049/68‑1 (MQ=255) caTGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGcc > 1:1147105/1‑68 (MQ=255) caTGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCt > 1:980497/1‑69 (MQ=255) caTGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCt > 1:1325913/1‑69 (MQ=255) caTGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCt > 1:3262612/1‑69 (MQ=255) aTGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTg < 1:944500/69‑1 (MQ=255) ttATCCGGGGAAGGAGTATCAGTTTGGCAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCg > 1:67493/1‑69 (MQ=255) ttATCCGGGGAAGGAGTATCAGTTTGGCAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCg > 1:1768110/1‑69 (MQ=255) ttATCCGGGGAAGGAGTATCAGTTTGGCAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCg > 1:307941/1‑69 (MQ=255) ttATCCGGGGAAGGAGTATCAGTTTGGCAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCg > 1:1756966/1‑69 (MQ=255) ttATCCGGGGAAGGAGTATCAGTTTGGCAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCg > 1:1632986/1‑69 (MQ=255) tATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTgg < 1:3238235/57‑1 (MQ=255) tATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTgg < 1:1268467/57‑1 (MQ=255) tATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTgg < 1:3016252/57‑1 (MQ=255) tATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTgg < 1:3012315/57‑1 (MQ=255) tATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTgg < 1:2890198/57‑1 (MQ=255) tATCCGGGGAGTGAATATCTATTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTgg < 1:1107266/57‑1 (MQ=255) ccGGGGAAGGAGTATCAGTTTGGCAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCgtgt < 1:1395852/68‑1 (MQ=255) ccGGGGAAGGAGTATCAGTTTGGCAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCgtgt < 1:3393192/68‑1 (MQ=255) ccGGGGAAGGAGTATCAGTTTGGCAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCgtgt < 1:2413416/68‑1 (MQ=255) ccGGGGAAGGAGTATCAGTTTGGCAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCgtgt < 1:1937453/68‑1 (MQ=255) ccGGGGAAGGAGTATCAGTTTGGCAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCgtgt < 1:989294/68‑1 (MQ=255) cGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCgtg > 1:3302957/1‑66 (MQ=255) cGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCgtg > 1:1780682/1‑66 (MQ=255) gAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGaa < 1:685486/69‑1 (MQ=255) | ATATTATTGAAATCACTAACGACAACATAAAATATGCCATCGCACATGCAGATTATCCGGGGAGTGAATATCTCTTTGGTAAAGAAATAGCGGAGAGCGAATTACTCTGGCCTGTTGATCGTGTGCAGAA > minE/1829798‑1829927 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |