| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,830,549 | C→G | 57.8% | L127L (CTG→CTC) | ygbI ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,830,549 | 0 | C | G | 57.8% | 39.0 / 93.4 | 90 | L127L (CTG→CTC) | ygbI | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (28/10); new base G (20/32); total (48/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCATCCA > minE/1830481‑1830607 | tgAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCAcagtc > 1:1328707/1‑69 (MQ=255)tgAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCAcagtc > 1:1162703/1‑69 (MQ=255) taatTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCAcagtcagt < 1:2339182/68‑1 (MQ=255) taatTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCAcagtcagt < 1:3342900/68‑1 (MQ=255) taatTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCAcagtcagt < 1:2854415/68‑1 (MQ=255) taatTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCAcagtcagt < 1:1934094/68‑1 (MQ=255) tgtgCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTc < 1:1568662/69‑1 (MQ=255) gtgCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTcc < 1:1470244/69‑1 (MQ=255) gtgCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTcc < 1:3127902/69‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCAtgt < 1:2070439/68‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCAtgt < 1:2481601/68‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCAtgt < 1:2571043/68‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCAtgt < 1:2423443/68‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCAtgt < 1:1981067/68‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCAtgt < 1:2383126/68‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCAtgt < 1:2636586/68‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCAtgt < 1:2131038/68‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCAtgt < 1:1800320/68‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCAtgt < 1:2269570/68‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCAtgt < 1:407232/68‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCAtgt < 1:562092/68‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCAtgt < 1:583813/68‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCAtgt < 1:800953/68‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCAtgt < 1:3243689/68‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCAtgt < 1:940037/68‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCAtgt < 1:962571/68‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCAtgt < 1:1093662/68‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCAtgtg < 1:997508/69‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCAtgtg < 1:237981/69‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCAtgtg < 1:922773/69‑1 (MQ=255) aTTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCAtgtg < 1:2148164/69‑1 (MQ=255) ttACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCAtgtg < 1:2839428/68‑1 (MQ=255) aCTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGa > 1:858379/1‑67 (MQ=255) aCTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGa > 1:1044390/1‑67 (MQ=255) aCTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGa > 1:2270210/1‑67 (MQ=255) aCTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGa > 1:985416/1‑67 (MQ=255) aCTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGa > 1:2633376/1‑67 (MQ=255) aCTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGa > 1:62768/1‑67 (MQ=255) aCTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGa > 1:2864448/1‑67 (MQ=255) aCTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGa > 1:331916/1‑67 (MQ=255) aCTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGa > 1:129023/1‑67 (MQ=255) aCTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGa > 1:1510162/1‑67 (MQ=255) aCTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGa > 1:1921332/1‑67 (MQ=255) aCTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGa > 1:2208481/1‑67 (MQ=255) aCTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGa > 1:1940684/1‑67 (MQ=255) aCTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGa > 1:2057701/1‑67 (MQ=255) aCTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGa > 1:1250176/1‑67 (MQ=255) aTAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTg < 1:390025/69‑1 (MQ=255) aTAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTg < 1:2206125/69‑1 (MQ=255) aTAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTg < 1:828222/69‑1 (MQ=255) aTAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTg < 1:3090915/69‑1 (MQ=255) aTAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTg < 1:1508819/69‑1 (MQ=255) aTAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTg < 1:1457322/69‑1 (MQ=255) aTAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTg < 1:2750711/69‑1 (MQ=255) aTAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTg < 1:977110/69‑1 (MQ=255) aTAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTg < 1:2695364/69‑1 (MQ=255) aTAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTg < 1:378320/69‑1 (MQ=255) aTAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTg < 1:1147116/69‑1 (MQ=255) aTAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTg < 1:2094056/69‑1 (MQ=255) aTAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTg < 1:1420424/69‑1 (MQ=255) tAACGAAGTCGTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTg < 1:2077556/68‑1 (MQ=255) aaCGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTgg > 1:1865076/1‑68 (MQ=255) aaCGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTgg > 1:2488163/1‑68 (MQ=255) aaCGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGt > 1:666752/1‑69 (MQ=255) aaCGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGt > 1:49881/1‑69 (MQ=255) aaCGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGt > 1:878650/1‑69 (MQ=255) aaCGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGt > 1:883826/1‑69 (MQ=255) aaCGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGt > 1:1924814/1‑69 (MQ=255) aaCGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGt > 1:929666/1‑69 (MQ=255) aaCGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGt > 1:1048544/1‑69 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCga > 1:961298/1‑68 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCGc > 1:787005/1‑69 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCGc > 1:1364505/1‑69 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCGc > 1:1324251/1‑69 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCGc > 1:3423471/1‑69 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCGc > 1:380608/1‑69 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCGc > 1:2366124/1‑69 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCGc > 1:237317/1‑69 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCGc > 1:2174044/1‑69 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCGc > 1:2400759/1‑69 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCGc > 1:2426018/1‑69 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCGc > 1:205491/1‑69 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCGc > 1:2748468/1‑69 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCGc > 1:2750659/1‑69 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCGc > 1:1825498/1‑69 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCGc > 1:1736090/1‑69 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCGc > 1:3188365/1‑69 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCGc > 1:1576200/1‑69 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCGc > 1:3430954/1‑69 (MQ=255) gTTAGTAACCACTGTGAGTGGCTCCATGTGAGTCAGATGCTGTGCAATCGCTAACGTGGTCGTTCCCGc > 1:1461198/1‑69 (MQ=255) tgtgACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCa > 1:922828/1‑67 (MQ=255) tgtgACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCa > 1:664110/1‑67 (MQ=255) tgtgACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCa > 1:1419728/1‑67 (MQ=255) tgtgACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCa > 1:1027947/1‑67 (MQ=255) gACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCATCCa < 1:821871/68‑1 (MQ=255) gACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCATCCa < 1:2460809/68‑1 (MQ=255) | TGAATAATTGTGCAATTACTGTTGTCGAGCAAGTAGTCCGCAATAACGAAATCGTTTGTGACCACAGTCAGTGACTCCATGTGAATCAGATGCTGTGCTATCGCTAACGTGGTCGTTCCCGCATCCA > minE/1830481‑1830607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |