| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,830,840 | G→A | 53.2% | V30V (GTC→GTT) | ygbI ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,830,840 | 0 | G | A | 53.2% | 8.6 / 97.7 | 111 | V30V (GTC→GTT) | ygbI | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (46/6); new base A (19/40); total (65/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.84e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
Only 100 of 118 total aligned reads displayed.
CCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTACGGGTATCAACTTTTGC > minE/1830786‑1830906 | ccGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTc > 1:2937282/1‑69 (MQ=255)ccGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTc > 1:271901/1‑69 (MQ=255)ccGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTc > 1:1751471/1‑69 (MQ=255)ccGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTc > 1:1579043/1‑69 (MQ=255)ccGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTc > 1:1455992/1‑69 (MQ=255) tGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCa > 1:1015270/1‑69 (MQ=255) tGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCa > 1:1889863/1‑69 (MQ=255) tGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCa > 1:1834197/1‑69 (MQ=255) tgtgACACATTCATTCTGTCCGTTAGTTCAGCAATACTAACAATGCCttt < 1:2947504/50‑1 (MQ=255) tgtgACACATTCATTCTGTCCGTTAGTTCAGCAATACTAACAATGCCttt < 1:27637/50‑1 (MQ=255) tgtgACACATTCATTCTGTCCGTTAGTTCAGCAATACTAACAATGCCttt < 1:3093414/50‑1 (MQ=255) tgtgACACATTCATTCTGTCCGTTAGTTCAGCAATACTAACAATGCCttt < 1:3425688/50‑1 (MQ=255) tgtgACACATTCATTCTGTCCGTTAGTTCAGCAATACTAACAATGCCttt < 1:1679123/50‑1 (MQ=255) tgtgACACATTCATTCTGTCCGTTAGTTCAGCAATACTAACAATGCCttt < 1:2990917/50‑1 (MQ=255) tgtgACACATTCATTCTGTCCGTTAGTTCAGCAATACTAACAATGCCttt < 1:646756/50‑1 (MQ=255) tgtgACACATTCATTCTGTCCGTTAGTTCAGCAATACTAACAATGCCttt < 1:1544994/50‑1 (MQ=255) tgtgACACATTCATTCTGTCCGTTAGTTCAGCAATACTAACAATGCCttt < 1:2656029/50‑1 (MQ=255) tgtgACACATTCATTCTGTCCGTTAGTTCAGCAATACTAACAATGCCttt < 1:769230/50‑1 (MQ=255) tgtgACACATTCATTCTGTCCGTTAGTTCAGCAATACTAACAATGCCttt < 1:292876/50‑1 (MQ=255) acacATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGgatga < 1:730174/69‑1 (MQ=255) acacATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGgatga < 1:2636054/69‑1 (MQ=255) acacATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGgatga < 1:919855/69‑1 (MQ=255) acacATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGgatca < 1:2361042/69‑3 (MQ=255) acacATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGgatca < 1:2356977/69‑3 (MQ=255) cacaTTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGgatga < 1:3373688/68‑1 (MQ=255) attcattcTGTCCGTTAGTTCAGCAATACTAACAATGCCttt < 1:310586/42‑1 (MQ=255) attcattcTGTCCGTTAGTTCAGCAATACTAACAATGCCt > 1:801466/1‑40 (MQ=255) attcattcTGTCCGTTAGTTCAGCAATACTAACAATGCCTTTTTCa > 1:1095864/1‑46 (MQ=255) attcattcTGTCCGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCt > 1:1804865/1‑49 (MQ=255) attcattcTGTCCGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGAttt > 1:3362585/1‑68 (MQ=255) attcattcTGTCCGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGAttt > 1:1381599/1‑68 (MQ=255) attcattcTGTCCGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTg > 1:3234339/1‑69 (MQ=255) attcattcTGTCCGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTg > 1:2483901/1‑69 (MQ=255) attcattcTGTCCGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTg > 1:2425837/1‑69 (MQ=255) attcattcTGTCCGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTg > 1:2407551/1‑69 (MQ=255) attcattcTGTCCGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTg > 1:1345995/1‑69 (MQ=255) attcattcTGTCCGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTg > 1:497098/1‑69 (MQ=255) attcattcTGTCCGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTg > 1:2308561/1‑69 (MQ=255) attcattcTGTCCGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTg > 1:2150686/1‑69 (MQ=255) attcattcTGTCCGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTg > 1:2567702/1‑69 (MQ=255) attcattcTGTCCGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTg > 1:364675/1‑69 (MQ=255) attcattcTGTCCGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTg > 1:1100600/1‑69 (MQ=255) ttcattcTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGAttt > 1:2473988/1‑67 (MQ=255) ttcattcTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTg > 1:2129393/1‑68 (MQ=255) ttcattcTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTg > 1:200995/1‑68 (MQ=255) ttcattcTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTg > 1:2365277/1‑68 (MQ=255) ttcattcTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTg > 1:3199247/1‑68 (MQ=255) ttcattcTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTg > 1:3290942/1‑68 (MQ=255) tcTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACg > 1:1910560/1‑69 (MQ=255) tcTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACg > 1:2948338/1‑69 (MQ=255) tcTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACg > 1:611490/1‑69 (MQ=255) tcTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACg > 1:1521152/1‑69 (MQ=255) tcTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACg > 1:793972/1‑69 (MQ=255) tcTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACg > 1:921068/1‑69 (MQ=255) tGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGc > 1:2117703/1‑68 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:2926015/69‑1 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:2933596/69‑1 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:1008455/69‑1 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:3269747/69‑1 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:338090/69‑1 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:671289/69‑1 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:859137/69‑1 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:905380/69‑1 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:187910/69‑1 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:2920250/69‑1 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:1003205/69‑1 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:2726337/69‑1 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:1117366/69‑1 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:1128810/69‑1 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:1157689/69‑1 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:1647382/69‑1 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:1169531/69‑1 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:2027888/69‑1 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:1511262/69‑1 (MQ=255) cGTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:1582316/69‑1 (MQ=255) gTTAGTTCAGCAATACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:183643/68‑1 (MQ=255) tACTAACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTAc < 1:234555/55‑1 (MQ=255) aCTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTACGGGTATCAACtttt > 1:109950/1‑68 (MQ=255) aCTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTACGGGTATCAACTTTTg > 1:2367888/1‑69 (MQ=255) aCTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTACGGGTATCAACTTTTg > 1:327257/1‑69 (MQ=255) aCTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTACGGGTATCAACTTTTg > 1:301949/1‑69 (MQ=255) aCTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTACGGGTATCAACTTTTg > 1:2788177/1‑69 (MQ=255) ccgACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGc > 1:94170/3‑43 (MQ=255) cTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTgg > 1:2119483/1‑42 (MQ=255) cTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGc > 1:2779871/1‑43 (MQ=255) cTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGc > 1:2036046/1‑43 (MQ=255) cTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGc > 1:1570205/1‑43 (MQ=255) cTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGc > 1:3410143/1‑43 (MQ=255) cTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTACGGGTATCAACtttt > 1:2727184/1‑67 (MQ=255) cTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTACGGGTATCAACTTTTGc > 1:1163212/1‑69 (MQ=255) cTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTACGGGTATCAACTTTTGc > 1:2662267/1‑69 (MQ=255) cTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTACGGGTATCAACTTTTGc > 1:2635722/1‑69 (MQ=255) cTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTACGGGTATCAACTTTTGc > 1:751821/1‑69 (MQ=255) cTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTACGGGTATCAACTTTTGc > 1:662114/1‑69 (MQ=255) gACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTa < 1:2468483/50‑1 (MQ=255) gACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTa < 1:1151510/50‑1 (MQ=255) gACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTa < 1:2608594/50‑1 (MQ=255) gACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTa < 1:895767/50‑1 (MQ=255) gACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTa < 1:2084241/50‑1 (MQ=255) gACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTa < 1:2011908/50‑1 (MQ=255) | CCGACGAATGGTCATATGTGACACATTCATTCTGTCCGTTAGTTCAGCAATACTGACAATGCCTTTTTCAGCTACCATCTCAAGGATGATTTGGCGACGCTCTACGGGTATCAACTTTTGC > minE/1830786‑1830906 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |