Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,831,039 A→C 44.5% intergenic (‑110/‑56) ygbI ← / → ygbJ predicted DNA‑binding transcriptional regulator/predicted dehydrogenase, with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,831,0390AC44.5% 21.1 / 158.6 144intergenic (‑110/‑56)ygbI/ygbJpredicted DNA‑binding transcriptional regulator/predicted dehydrogenase, with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base A (47/33);  new base C (51/13);  total (98/46)
Fisher's exact test for biased strand distribution p-value = 1.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

TCACATAAAATAACACACAATGTTAATTTATGTGAATCAGATCACCATACCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGG  >  minE/1830978‑1831105
                                                             |                                                                  
tCACATAAAATAACACGCAATGTTAATTTTTGTGAATCAGCTCACCATATCGTTATCTTTCCGCGCTta                                                             <  1:1022803/69‑1 (MQ=255)
tCACATAAAATAACACGCAATGTTAATTTTTGTGAATCAGCTCACCATATCGTTATCTTTCCGCGCTta                                                             <  1:355251/69‑1 (MQ=255)
         aTAACATGCAATGTTAATTTTTGTGAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTCACaa                                                    >  1:671087/1‑69 (MQ=255)
         aTAACACGCAATTTTAATTTTTGTGAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTCACaa                                                    >  1:2611760/1‑69 (MQ=255)
         aTAACACGCAATGTTAATTTTTGTGAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTcaca                                                     >  1:2578931/1‑68 (MQ=255)
         aTAACACGCAATGTTAATTTTTGTGAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTc                                                        >  1:2716316/1‑65 (MQ=255)
         aTAACACGCAATGTTAATTTTTGTGAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTCACaa                                                    >  1:2233145/1‑69 (MQ=255)
         aTAACACGCAATGTTAATTTTTGTGAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTCACaa                                                    >  1:786713/1‑69 (MQ=255)
         aTAACACGCAATGTTAATTTTTGTGAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTCACaa                                                    >  1:394123/1‑69 (MQ=255)
         aTAACACGCAATGTTAATTTTTGTGAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTCACaa                                                    >  1:2926780/1‑69 (MQ=255)
         aTAACACGCAATGTTAATTTTTGTGAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTCACaa                                                    >  1:1098141/1‑69 (MQ=255)
         aTAACACGCAATGTTAATTTTTGTGAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTCACaa                                                    >  1:3260102/1‑69 (MQ=255)
         aTAACACGCAATGTTAATTTTTGTGAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTCACaa                                                    >  1:1871477/1‑69 (MQ=255)
         aTAACACGCAATGTTAATTTTTGTGAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTCACaa                                                    >  1:1526880/1‑69 (MQ=255)
         aTAACACGCAATGTTAATTTTTGTGAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTCACaa                                                    >  1:3243821/1‑69 (MQ=255)
         aTAACACGCAATGTTAATTTTTGTGAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTCACaa                                                    >  1:3106731/1‑69 (MQ=255)
         aTAACACGCAATGTTAATTTTTGTGAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTCACaa                                                    >  1:3211454/1‑69 (MQ=255)
         aTAACACACAATGTTAATTTATGTGAATCAGATCACCATACCGTTATCTTCCAGCGCTTATATTCACaa                                                    <  1:28907/69‑1 (MQ=255)
                   aTGTTAATTTATGTGAATCAGATCACCATACCGTTATCTTCCAGCGCTTATATTCACAATATcaaacaa                                          <  1:1086275/69‑1 (MQ=255)
                      ttAATTTATGTGAATCAGATCACCATACCGTTATCTTCCAGCGCTTATATTCACAATATCAAACaaaa                                        >  1:510619/1‑68 (MQ=255)
                      ttAATTTATGTGAATCAGATCACCATACCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAat                                       >  1:1719606/1‑69 (MQ=255)
                      ttAATTTATGTGAATCAGATCACCATACCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAat                                       <  1:1660879/69‑1 (MQ=255)
                      ttAATTTATGTGAATCAGATCACCATACCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAat                                       <  1:2878562/69‑1 (MQ=255)
                      ttAATTTATGTGAATCAGATCACCATACCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAat                                       <  1:1950782/69‑1 (MQ=255)
                      ttAATTTATGTGAATCAGATCACCATACCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAat                                       <  1:2484158/69‑1 (MQ=255)
                          tttATGTGAATCAGATCACCATACCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATc                                    <  1:2731427/68‑1 (MQ=255)
                          tttATGTGAATCAGATCACCATACCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATc                                    <  1:2408791/68‑1 (MQ=255)
                          tttATGTGAATCAGATCACCATACCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATc                                    <  1:1413835/68‑1 (MQ=255)
                          tttATGTGAATCAGATCACCATACCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATc                                    <  1:2051324/68‑1 (MQ=255)
                          tttATGTGAATCAGATCACCATACCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATc                                    <  1:1190144/68‑1 (MQ=255)
                          tttATGTGAATCAGATCACCATACCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATc                                    <  1:2127457/68‑1 (MQ=255)
                           ttttgtgAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCa                                   <  1:2390032/65‑1 (MQ=255)
                           ttttgtgAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCa                                   <  1:1971586/65‑1 (MQ=255)
                           ttttgtgAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCa                                   <  1:2203001/65‑1 (MQ=255)
                           ttttgtgAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCa                                   <  1:2951066/65‑1 (MQ=255)
                           ttttgtgAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCa                                   <  1:3012459/65‑1 (MQ=255)
                           ttttgtgAATCAGCTCACCATATCGTTATCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCa                                   <  1:1500003/65‑1 (MQ=255)
                                          cACCATACCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAg                     >  1:2923825/1‑67 (MQ=255)
                                          cACCATACCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAg                     >  1:2157790/1‑67 (MQ=255)
                                          cACCATACCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAg                     >  1:2836933/1‑67 (MQ=255)
                                          cACCATACCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAg                     >  1:2960712/1‑67 (MQ=255)
                                                aCCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAg             <  1:2230997/69‑1 (MQ=255)
                                                aCCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAg             <  1:2254648/69‑1 (MQ=255)
                                                aCCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAg             <  1:2373285/69‑1 (MQ=255)
                                                aCCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAg             <  1:484685/69‑1 (MQ=255)
                                                 ccGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAat                                       >  1:2079388/1‑42 (MQ=255)
                                                 ccGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAg             >  1:280161/1‑68 (MQ=255)
                                                 ccGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAg             >  1:3341260/1‑68 (MQ=255)
                                                 ccGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAg             >  1:341168/1‑68 (MQ=255)
                                                 ccGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAg             >  1:256822/1‑68 (MQ=255)
                                                 ccGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAg             >  1:1147332/1‑68 (MQ=255)
                                                 ccGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAg             >  1:1413907/1‑68 (MQ=255)
                                                 ccGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAg             >  1:1372247/1‑68 (MQ=255)
                                                 ccGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAg             >  1:2170759/1‑68 (MQ=255)
                                                 cagTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAg             >  1:782069/3‑68 (MQ=255)
                                                    ttATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATg          >  1:2000797/1‑68 (MQ=255)
                                                     tATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaa        <  1:2696041/69‑1 (MQ=255)
                                                     tATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaa        <  1:585218/69‑1 (MQ=255)
                                                     tATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaa        <  1:3410014/69‑1 (MQ=255)
                                                     tATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaa        <  1:3413786/69‑1 (MQ=255)
                                                      aTCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCACTTa                               >  1:3058046/1‑45 (MQ=38)
                                                      aTCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGa            >  1:2254534/1‑64 (MQ=255)
                                                      aTCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:1311185/1‑69 (MQ=255)
                                                      aTCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:1688439/1‑69 (MQ=255)
                                                      aTCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:1476261/1‑69 (MQ=255)
                                                      aTCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:1581602/1‑69 (MQ=255)
                                                      aTCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:449762/1‑69 (MQ=255)
                                                      aTCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:1231684/1‑69 (MQ=255)
                                                      aTCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:682511/1‑69 (MQ=255)
                                                      aTCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:933086/1‑69 (MQ=255)
                                                      aTCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:958591/1‑69 (MQ=255)
                                                      aTCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:2507601/1‑69 (MQ=255)
                                                      aTCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:2441234/1‑69 (MQ=255)
                                                      aTCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:2377483/1‑69 (MQ=255)
                                                      aTCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:279164/1‑69 (MQ=255)
                                                      aTCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:1792964/1‑69 (MQ=255)
                                                      aTCTTTCCGCGCTTATATTCACAACATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:2203540/1‑69 (MQ=255)
                                                      aTCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:2282404/1‑69 (MQ=255)
                                                      aTCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:848608/1‑69 (MQ=255)
                                                      aTCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:1089646/1‑69 (MQ=255)
                                                      aTCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:631766/1‑69 (MQ=255)
                                                      aTCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:526355/1‑69 (MQ=255)
                                                      aTCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:903843/1‑69 (MQ=255)
                                                      aTCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:2340132/1‑69 (MQ=255)
                                                      aTCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:123392/1‑69 (MQ=255)
                                                      aTCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:2837784/1‑69 (MQ=255)
                                                      aTCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:1779261/1‑69 (MQ=255)
                                                      aTCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:3316121/1‑69 (MQ=255)
                                                      aTCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:3284355/1‑69 (MQ=255)
                                                      aTCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:2602310/1‑69 (MQ=255)
                                                      aTCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:1005983/1‑69 (MQ=255)
                                                      aTCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:324362/1‑69 (MQ=255)
                                                      aTCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:3069736/1‑69 (MQ=255)
                                                      aTCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaaa       >  1:1719952/1‑69 (MQ=255)
                                                      aTCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaa        >  1:2521247/1‑68 (MQ=255)
                                                      aTCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGaa        <  1:1058178/68‑1 (MQ=255)
                                                         ttCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAAc     >  1:3165219/1‑68 (MQ=255)
                                                             aGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACggg  <  1:2681257/67‑1 (MQ=255)
                                                             aGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACggg  <  1:465044/67‑1 (MQ=255)
                                                             aGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACggg  <  1:177665/67‑1 (MQ=255)
                                                             |                                                                  
TCACATAAAATAACACACAATGTTAATTTATGTGAATCAGATCACCATACCGTTATCTTCCAGCGCTTATATTCACAATATCAAACAAAATATCACTTAAATTAACAAGGAGAGCAGATGAAAACGGG  >  minE/1830978‑1831105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: