| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,833,617 | T→G | 39.1% | R120R (CGT→CGG) | ygbL → | predicted class II aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,833,617 | 0 | T | G | 39.1% | 42.5 / 86.6 | 92 | R120R (CGT→CGG) | ygbL | predicted class II aldolase |
| Reads supporting (aligned to +/- strand): ref base T (33/23); new base G (14/22); total (47/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.71e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTGGTGC > minE/1833549‑1833666 | cATTTGCACAGCACATGGTCGCCGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGt > 1:2636582/1‑69 (MQ=255)cATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGt > 1:2569153/1‑69 (MQ=255)cATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGt > 1:1845958/1‑69 (MQ=255) tttGCACAGCTCATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTc < 1:2962585/68‑1 (MQ=255) tttGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTc < 1:2468656/68‑1 (MQ=255) tGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCg > 1:347206/1‑68 (MQ=255) tGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCg > 1:2236570/1‑68 (MQ=255) tGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCg > 1:2293553/1‑68 (MQ=255) tGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCg > 1:3187310/1‑68 (MQ=255) tGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCg > 1:3237513/1‑68 (MQ=255) tGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCg > 1:2964227/1‑68 (MQ=255) tGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCg > 1:1872370/1‑68 (MQ=255) tGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCg > 1:231448/1‑68 (MQ=255) tGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCg > 1:2412061/1‑68 (MQ=255) tGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCg > 1:1740306/1‑68 (MQ=255) tGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCg > 1:2480582/1‑68 (MQ=255) tGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCg > 1:500979/1‑68 (MQ=255) tGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCg > 1:622045/1‑68 (MQ=255) tGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCg > 1:2485871/1‑68 (MQ=255) tGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCg > 1:2620063/1‑68 (MQ=255) tGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCg > 1:875469/1‑68 (MQ=255) tGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCg > 1:917567/1‑68 (MQ=255) tGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCg > 1:94353/1‑68 (MQ=255) tGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAG‑AACGTTATTCGTCCGtt > 1:574535/1‑69 (MQ=255) cacagcacaTGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGtt < 1:424461/68‑1 (MQ=255) cacagcacaTGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGtt < 1:509250/68‑1 (MQ=255) cacaTGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTc < 1:2892908/59‑1 (MQ=255) gTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACgt > 1:1957614/1‑69 (MQ=255) gTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACg > 1:2646170/1‑68 (MQ=255) cGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGtt < 1:3129939/55‑1 (MQ=255) gACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACgtggt < 1:3262000/69‑1 (MQ=255) gACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACgtggt < 1:705814/69‑1 (MQ=255) gACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACgtggt < 1:3315265/69‑1 (MQ=255) gACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACgtggt < 1:1164163/69‑1 (MQ=255) gACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACgtggt < 1:1547313/69‑1 (MQ=255) gACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACgtggt < 1:3375573/69‑1 (MQ=255) gACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACgtggt < 1:694165/69‑1 (MQ=255) tCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGtt < 1:2906665/44‑1 (MQ=255) cctggATGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:3037899/66‑1 (MQ=255) cctggAAGGGCTGGACAGCAATAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATggg < 1:2356289/66‑1 (MQ=255) cctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:2680150/66‑1 (MQ=255) cctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:2701986/66‑1 (MQ=255) cctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:703097/66‑1 (MQ=255) cctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:625190/66‑1 (MQ=255) cctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:3348477/66‑1 (MQ=255) cctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:3269779/66‑1 (MQ=255) cctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:3021804/66‑1 (MQ=255) cctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:1808342/66‑1 (MQ=255) cctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:1024304/66‑1 (MQ=255) cctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:1042625/66‑1 (MQ=255) cctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:1291244/66‑1 (MQ=255) cctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:1303097/66‑1 (MQ=255) cctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:1921900/66‑1 (MQ=255) cctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:20687/66‑1 (MQ=255) cctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:2223427/66‑1 (MQ=255) cctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:2227366/66‑1 (MQ=255) cctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:2310292/66‑1 (MQ=255) cctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:2487909/66‑1 (MQ=255) ctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:565849/65‑1 (MQ=255) ctggAAGGGCTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:2993796/65‑1 (MQ=255) cTGGACAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATggg < 1:3139358/56‑1 (MQ=255) aCAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATGGGTAATGTCCCGCTGGTg > 1:2012609/1‑68 (MQ=255) aCAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATGGGTAATGTCCCGCTGGTGc > 1:2946377/1‑69 (MQ=255) aCAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATGGGTAATGTCCCGCTGGTGc > 1:1273327/1‑69 (MQ=255) aCAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATGGGTAATGTCCCGCTGGTGc > 1:1340701/1‑69 (MQ=255) aCAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATGGGTAATGTCCCGCTGGTGc > 1:3376086/1‑69 (MQ=255) aCAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATGGGTAATGTCCCGCTGGTGc > 1:1217105/1‑69 (MQ=255) aCAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATGGGTAATGTCCCGCTGGTGc > 1:1778365/1‑69 (MQ=255) aCAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATGGGTAATGTCCCGCTGGTGc > 1:1005487/1‑69 (MQ=255) aCAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATGGGTAATGTCCCGCTGGTGc > 1:1116314/1‑69 (MQ=255) aCAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATGGGTAATGTCCCGCTGGTGc > 1:3166675/1‑69 (MQ=255) aCAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATGGGTAATGTCCCGCTGGTGc > 1:2570212/1‑69 (MQ=255) aCAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATGGGTAATGTCCCGCTGGTGc > 1:2920044/1‑69 (MQ=255) aCAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGATGGGTAATGTCCCGCTGGTGc > 1:2413124/1‑69 (MQ=255) aCAGCAATAACGTTATTCGGCCGTTCACACCATACGTGGTGATGCGGAT‑GGTAATGTCCCGCTGGTGc > 1:1245140/1‑68 (MQ=255) cagcagCAACGTTATTCGTCCGTTCACACCATACgt < 1:3212025/36‑1 (MQ=255) cagcagCAACGTTATTCGTCCGTTCACACCATACgt < 1:113613/36‑1 (MQ=255) cagcagCAACGTTATTCGTCCGTTCACACCATACgt < 1:3109926/36‑1 (MQ=255) cagcagCAACGTTATTCGTCCGTTCACACCATACgt < 1:1511492/36‑1 (MQ=255) cagcagCAACGTTATTCGTCCGTTCACACCATACgt < 1:699993/36‑1 (MQ=255) cagcagCAACGTTATTCGTCCGTTCACACCATACgt < 1:1243445/36‑1 (MQ=255) cagcagCAACGTTATTCGTCCGTTCACACCATACgt < 1:404869/36‑1 (MQ=255) cagcagCAACGTTATTCGTCCGTTCACACCATACgt < 1:1284490/36‑1 (MQ=255) cagcagCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTGGTGc > 1:2688603/1‑68 (MQ=255) cagcagCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTGGTGc > 1:515412/1‑68 (MQ=255) cagcagCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTGGTGc > 1:2748745/1‑68 (MQ=255) cagcagCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTGGTGc > 1:2803514/1‑68 (MQ=255) cagcagCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTGGTGc > 1:391886/1‑68 (MQ=255) cagcagCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTGGTGc > 1:1378116/1‑68 (MQ=255) cagcagCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTGGTGc > 1:3083613/1‑68 (MQ=255) gTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTgg > 1:635886/1‑55 (MQ=255) gTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTgg > 1:1590473/1‑55 (MQ=255) gTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTgg > 1:2840932/1‑55 (MQ=255) gTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTgg > 1:2531642/1‑55 (MQ=255) gTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTgg > 1:2288808/1‑55 (MQ=255) | CATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCTGGACAGCAGCAACGTTATTCGTCCGTTCACACCATACGTGGTGATGCGGATGGGAAATGTCCCGCTGGTGC > minE/1833549‑1833666 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |