| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,841,416 | A→G | 40.3% | H10H (CAT→CAC) | ispF ← | 2C‑methyl‑D‑erythritol 2,4‑cyclodiphosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,841,416 | 0 | A | G | 40.3% | 46.8 / 80.8 | 114 | H10H (CAT→CAC) | ispF | 2C‑methyl‑D‑erythritol 2,4‑cyclodiphosphate synthase |
| Reads supporting (aligned to +/- strand): ref base A (51/17); new base G (36/10); total (87/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 114 total aligned reads displayed.
AATCCTTTTTCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAA > minE/1841354‑1841481 | aaTCCTTTTTCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTAc > 1:1998791/1‑68 (MQ=255)aaTCCTTTTTCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTAc > 1:2962663/1‑68 (MQ=255)aaTCCTTTTTCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTAc > 1:2069536/1‑68 (MQ=255) ccTTTTTCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGt > 1:1625241/1‑67 (MQ=255) ccTTTTTCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTc > 1:513510/1‑68 (MQ=255) ccTTTTTCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTc > 1:2469650/1‑68 (MQ=255) ccTTTTTCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTc > 1:1927777/1‑68 (MQ=255) ccTTTTTCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTc > 1:2259945/1‑68 (MQ=255) ccTTTTTCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTc > 1:2579784/1‑68 (MQ=255) ccTTTTTCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTc > 1:3212695/1‑68 (MQ=255) ccTTTTTCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTc > 1:1068656/1‑68 (MQ=255) ttCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAAcc > 1:1236328/1‑69 (MQ=255) ttCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAAcc > 1:709233/1‑69 (MQ=255) ttCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAAcc > 1:2315977/1‑69 (MQ=255) ttCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAAcc > 1:1481950/1‑69 (MQ=255) ttCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAAcc > 1:1843217/1‑69 (MQ=255) ttCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAAcc > 1:2824566/1‑69 (MQ=255) ttCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAAc > 1:2931120/1‑68 (MQ=255) tGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAAtt > 1:1826023/1‑68 (MQ=255) tGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAAtt > 1:400634/1‑68 (MQ=255) tGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAAtt > 1:3155079/1‑68 (MQ=255) tGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAAtt > 1:110879/1‑68 (MQ=255) tGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAAtt > 1:1098323/1‑68 (MQ=255) tGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAAtt > 1:1066940/1‑68 (MQ=255) agCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAAtt > 1:656031/2‑68 (MQ=255) gCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCg > 1:1848008/1‑69 (MQ=255) gCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCg > 1:175550/1‑69 (MQ=255) gCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCg > 1:763268/1‑69 (MQ=255) gCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCg > 1:3101190/1‑69 (MQ=255) tACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCAt < 1:424418/69‑1 (MQ=255) tACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCAt < 1:420436/69‑1 (MQ=255) tACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCAt < 1:410906/69‑1 (MQ=255) tACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCAt < 1:2373069/69‑1 (MQ=255) tACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCAt < 1:2940818/69‑1 (MQ=255) tACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCAt < 1:342167/69‑1 (MQ=255) tACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCAt < 1:3247672/69‑1 (MQ=255) tACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCAt < 1:2186285/69‑1 (MQ=255) tACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCAt < 1:685286/69‑1 (MQ=255) tACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCAt < 1:1374248/69‑1 (MQ=255) tACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCAt < 1:943144/69‑1 (MQ=255) tACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCAt < 1:1130097/69‑1 (MQ=255) tACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCAt < 1:2026098/69‑1 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAAcc > 1:2017572/1‑51 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCaa > 1:3184086/1‑59 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCa > 1:942512/1‑58 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCa > 1:1701847/1‑65 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCAt > 1:333817/1‑66 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCAt > 1:3216929/1‑66 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCAt > 1:3203449/1‑66 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCAt > 1:1237564/1‑66 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCAt > 1:3018998/1‑66 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCAt > 1:378544/1‑66 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCAt > 1:1240149/1‑66 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCAt > 1:3336571/1‑66 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCAt > 1:2937575/1‑66 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCAt > 1:878895/1‑66 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCAt > 1:83877/1‑66 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCAt > 1:2407419/1‑66 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCAt > 1:2424792/1‑66 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCAt > 1:2441422/1‑66 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCAt > 1:1975654/1‑66 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCAt > 1:1917304/1‑66 (MQ=255) gCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTAGCAt > 1:2516168/1‑66 (MQ=255) aTTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTga > 1:1783897/1‑68 (MQ=255) aTTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTga > 1:864127/1‑68 (MQ=255) aTTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTg > 1:2833578/1‑67 (MQ=255) ccTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATg > 1:832747/1‑51 (MQ=255) ccTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTgatggatg > 1:1843739/1‑68 (MQ=255) ccTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTgatggatg > 1:1238512/1‑68 (MQ=255) ccTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTgatggatg > 1:473463/1‑68 (MQ=255) ccTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTgatggatg > 1:3376052/1‑68 (MQ=255) ccTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGatggatgg > 1:1060907/1‑69 (MQ=255) ccTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGatggatgg > 1:612779/1‑69 (MQ=255) ccTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGatggatgg > 1:966233/1‑69 (MQ=255) ccTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGatggatgg > 1:2339973/1‑69 (MQ=255) ccTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGatggatgg > 1:1103151/1‑69 (MQ=255) ccTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGatggatgg > 1:1700727/1‑69 (MQ=255) ccTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGatggatgg > 1:2010209/1‑69 (MQ=255) ccTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGatggatgg > 1:2608249/1‑69 (MQ=255) ccTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGatggatgg > 1:2644691/1‑69 (MQ=255) ccTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGatggatgg > 1:295480/1‑69 (MQ=255) cGCCAAATGCGTGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCggg < 1:646117/68‑1 (MQ=255) cGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCggg < 1:405430/68‑1 (MQ=255) cGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCggg < 1:2921337/68‑1 (MQ=255) cGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCggg < 1:2906338/68‑1 (MQ=255) cGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCggg < 1:2631083/68‑1 (MQ=255) cGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCggg < 1:369058/68‑1 (MQ=255) cGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCggg < 1:1142450/68‑1 (MQ=255) cGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCggg < 1:2104164/68‑1 (MQ=255) cGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCggg < 1:2036288/68‑1 (MQ=255) cGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCgg > 1:3349100/1‑67 (MQ=255) cGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCgg > 1:138177/1‑67 (MQ=255) cGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCgg > 1:156380/1‑67 (MQ=255) cGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCgg > 1:3262624/1‑67 (MQ=255) cGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCgg > 1:3057240/1‑67 (MQ=255) cGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCgg > 1:2170253/1‑67 (MQ=255) cGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCgg > 1:2193460/1‑67 (MQ=255) cGCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTAGCATTATGTATTCTCCTGATGGATGGTTCgg > 1:1340719/1‑67 (MQ=255) gCCAAAGGCGTGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCggg < 1:3318343/67‑1 (MQ=255) ggCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTaa < 1:3007635/69‑1 (MQ=255) | AATCCTTTTTCGTAAGGAATGCGTACGCCACCAATGATAATTGGGCCTTCACCGCCAAAGGCATGTACGTCAAAACCGTGTCCAATTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAA > minE/1841354‑1841481 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |