Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,841,493 T→C 42.6% A221A (GCA→GCG ispD ← 4‑diphosphocytidyl‑2C‑methyl‑D‑erythritol synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,841,4930TC42.6% 19.4 / 56.5 75A221A (GCA→GCGispD4‑diphosphocytidyl‑2C‑methyl‑D‑erythritol synthase
Reads supporting (aligned to +/- strand):  ref base T (31/12);  new base C (23/9);  total (54/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

CAATTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAA  >  minE/1841436‑1841546
                                                         |                                                     
cAATTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCt                                              >  1:878949/1‑67 (MQ=255)
cAATTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCt                                              >  1:2777025/1‑67 (MQ=255)
cAATTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCt                                              >  1:840747/1‑67 (MQ=255)
cAATTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCt                                              >  1:2474971/1‑67 (MQ=255)
  aTTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATc                                               >  1:267424/1‑64 (MQ=255)
  aTTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATc                                               >  1:1991012/1‑64 (MQ=255)
  aTTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATc                                               >  1:202976/1‑64 (MQ=255)
  aTTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATc                                               >  1:1837430/1‑64 (MQ=255)
  aTTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATc                                               >  1:379844/1‑64 (MQ=255)
  aTTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATc                                               >  1:649195/1‑64 (MQ=255)
  aTTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATc                                               >  1:2459188/1‑64 (MQ=255)
  aTTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATc                                               >  1:3318173/1‑64 (MQ=255)
  aTTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATc                                               >  1:25846/1‑64 (MQ=255)
  aTTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATc                                               >  1:1560011/1‑64 (MQ=255)
  aTTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATc                                               >  1:1289065/1‑64 (MQ=255)
  aTTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATc                                               >  1:2823016/1‑64 (MQ=255)
  aTTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATc                                               >  1:2964404/1‑64 (MQ=255)
  aTTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATc                                               >  1:3031172/1‑64 (MQ=255)
           aTGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGa                                 >  1:758886/1‑69 (MQ=255)
           aTGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGa                                 >  1:145882/1‑69 (MQ=255)
           aTGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGa                                 >  1:2805568/1‑69 (MQ=255)
           aTGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGAGTGa                                 >  1:630264/1‑69 (MQ=255)
                ttCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACttt                             <  1:133869/68‑1 (MQ=255)
                ttCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACttt                             <  1:861917/68‑1 (MQ=255)
                ttCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACttt                             <  1:2762430/68‑1 (MQ=255)
                ttCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACttt                             <  1:572033/68‑1 (MQ=255)
                ttCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACttt                             <  1:1834461/68‑1 (MQ=255)
                ttCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACttt                             <  1:2313441/68‑1 (MQ=255)
                ttCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACttt                             <  1:3030428/68‑1 (MQ=255)
                ttCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACttt                             <  1:2512018/68‑1 (MQ=255)
                ttCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACttt                             <  1:656536/68‑1 (MQ=255)
                  ctcCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACcttga                           >  1:1222211/1‑66 (MQ=255)
                  ctcCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGAt                          >  1:328644/1‑69 (MQ=255)
                  ctcCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGAt                          >  1:2775300/1‑69 (MQ=255)
                  ctcCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGAt                          >  1:2697066/1‑69 (MQ=255)
                  ctcCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGAt                          >  1:2838479/1‑69 (MQ=255)
                  ctcCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGAt                          >  1:520910/1‑69 (MQ=255)
                  ctcCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGAt                          >  1:211163/1‑69 (MQ=255)
                  ctcCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGAt                          >  1:1903170/1‑69 (MQ=255)
                  ctcCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGAt                          >  1:1928250/1‑69 (MQ=255)
                  ctcCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTatt                          >  1:1911508/1‑67 (MQ=255)
                  ctcCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAAt                          >  1:1199881/1‑69 (MQ=255)
                  ctcCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAAt                          >  1:715024/1‑69 (MQ=255)
                  ctcCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAAt                          >  1:1569500/1‑69 (MQ=255)
                  ctcCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAAt                          >  1:2127385/1‑69 (MQ=255)
                  ctcCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAAt                          >  1:2754830/1‑69 (MQ=255)
                  ctcCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAAt                          >  1:1032340/1‑69 (MQ=255)
                       gatggatgGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTTATGt                        <  1:1934872/66‑1 (MQ=255)
                       gatggatgGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGATGt                        <  1:695253/66‑1 (MQ=255)
                       gatggatgGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGATGt                        <  1:652165/66‑1 (MQ=255)
                       gatggatgGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGATGt                        <  1:3420620/66‑1 (MQ=255)
                       gatggatgGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGATGt                        <  1:1118960/66‑1 (MQ=255)
                       gatggatgGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGATGt                        <  1:2220846/66‑1 (MQ=255)
                       gatggatgGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGATGt                        <  1:1629730/66‑1 (MQ=255)
                       gatggatgGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGATGt                        <  1:173657/66‑1 (MQ=255)
                       gatggatgGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGATGt                        <  1:1785901/66‑1 (MQ=255)
                       gatggatgGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGt                        <  1:3056518/66‑1 (MQ=255)
                       gatggatgGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGt                        <  1:1416965/66‑1 (MQ=255)
                       gatggatgGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGt                        <  1:1696548/66‑1 (MQ=255)
                             tggTTCGGGTGAGGTAAACCTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCa                >  1:2091343/1‑68 (MQ=255)
                             tggTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCa                >  1:570844/1‑68 (MQ=255)
                             tggTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCAc               >  1:868597/1‑69 (MQ=255)
                             tggTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCAc               >  1:2534456/1‑69 (MQ=255)
                             tggTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCAc               >  1:1759091/1‑69 (MQ=255)
                                 tCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATg                         >  1:323935/1‑55 (MQ=255)
                                 tCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGtt                       >  1:1269606/1‑57 (MQ=255)
                                 tCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGtt                       >  1:2394605/1‑57 (MQ=255)
                                 tCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGtt                       >  1:2826722/1‑57 (MQ=255)
                                            aaaaCTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACg              >  1:1609878/1‑55 (MQ=255)
                                            aaaaCTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCaa  >  1:2153879/1‑67 (MQ=255)
                                            aaaaCTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCaa  >  1:398428/1‑67 (MQ=255)
                                            aaaaCTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCaa  >  1:824110/1‑67 (MQ=255)
                                            aaaaCTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCaa  >  1:1534892/1‑67 (MQ=255)
                                            aaaaCTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCaa  >  1:3092610/1‑67 (MQ=255)
                                            aaaaCTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCGTCGACCaa  >  1:2112882/1‑67 (MQ=255)
                                                         |                                                     
CAATTCGCATTATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAA  >  minE/1841436‑1841546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: