| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,841,514 | G→T | 23.0% | V214V (GTC→GTA) | ispD ← | 4‑diphosphocytidyl‑2C‑methyl‑D‑erythritol synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,841,514 | 0 | G | T | 23.0% | 138.2 / 30.8 | 78 | V214V (GTC→GTA) | ispD | 4‑diphosphocytidyl‑2C‑methyl‑D‑erythritol synthase |
| Reads supporting (aligned to +/- strand): ref base G (43/17); new base T (9/9); total (52/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.75e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
ATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGAATCCGCAATATTCCAGCGCC > minE/1841447‑1841577 | aTGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGa > 1:145882/1‑69 (MQ=255)aTGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGa > 1:758886/1‑69 (MQ=255)aTGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGa > 1:2805568/1‑69 (MQ=255)aTGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGAGTGa > 1:630264/1‑69 (MQ=255) ttCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACttt < 1:133869/68‑1 (MQ=255) ttCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACttt < 1:1834461/68‑1 (MQ=255) ttCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACttt < 1:3030428/68‑1 (MQ=255) ttCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACttt < 1:572033/68‑1 (MQ=255) ttCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACttt < 1:656536/68‑1 (MQ=255) ttCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACttt < 1:2512018/68‑1 (MQ=255) ttCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACttt < 1:2313441/68‑1 (MQ=255) ttCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACttt < 1:861917/68‑1 (MQ=255) ttCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACttt < 1:2762430/68‑1 (MQ=255) ctcCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACcttga > 1:1222211/1‑66 (MQ=255) ctcCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGAt > 1:2697066/1‑69 (MQ=255) ctcCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGAt > 1:2775300/1‑69 (MQ=255) ctcCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGAt > 1:2838479/1‑69 (MQ=255) ctcCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGAt > 1:328644/1‑69 (MQ=255) ctcCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGAt > 1:1903170/1‑69 (MQ=255) ctcCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGAt > 1:520910/1‑69 (MQ=255) ctcCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGAt > 1:1928250/1‑69 (MQ=255) ctcCTGATGGATGGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGAt > 1:211163/1‑69 (MQ=255) ctcCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTatt > 1:1911508/1‑67 (MQ=255) ctcCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAAt > 1:2127385/1‑69 (MQ=255) ctcCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAAt > 1:2754830/1‑69 (MQ=255) ctcCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAAt > 1:1569500/1‑69 (MQ=255) ctcCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAAt > 1:715024/1‑69 (MQ=255) ctcCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAAt > 1:1199881/1‑69 (MQ=255) ctcCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAAt > 1:1032340/1‑69 (MQ=255) gatggatgGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTTATGt < 1:1934872/66‑1 (MQ=255) gatggatgGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGATGt < 1:3420620/66‑1 (MQ=255) gatggatgGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGATGt < 1:652165/66‑1 (MQ=255) gatggatgGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGATGt < 1:695253/66‑1 (MQ=255) gatggatgGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGATGt < 1:2220846/66‑1 (MQ=255) gatggatgGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGATGt < 1:1118960/66‑1 (MQ=255) gatggatgGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGATGt < 1:1629730/66‑1 (MQ=255) gatggatgGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGATGt < 1:173657/66‑1 (MQ=255) gatggatgGTTCGGGTGAGGTAAAATTCGGCCAGCGCCAAATCCTCCGGGCGCGTTACCTTGATGt < 1:1785901/66‑1 (MQ=255) gatggatgGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGt < 1:3056518/66‑1 (MQ=255) gatggatgGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGt < 1:1416965/66‑1 (MQ=255) gatggatgGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGt < 1:1696548/66‑1 (MQ=255) tggTTCGGGTGAGGTAAACCTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCa > 1:2091343/1‑68 (MQ=255) tggTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCa > 1:570844/1‑68 (MQ=255) tggTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCAc > 1:868597/1‑69 (MQ=255) tggTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCAc > 1:1759091/1‑69 (MQ=255) tggTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCAc > 1:2534456/1‑69 (MQ=255) tCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATg > 1:323935/1‑55 (MQ=255) tCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGtt > 1:2394605/1‑57 (MQ=255) tCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGtt > 1:1269606/1‑57 (MQ=255) tCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGtt > 1:2826722/1‑57 (MQ=255) aaaaCTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACg > 1:1609878/1‑55 (MQ=255) aaaaCTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCaa > 1:3092610/1‑67 (MQ=255) aaaaCTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCaa > 1:2153879/1‑67 (MQ=255) aaaaCTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCaa > 1:1534892/1‑67 (MQ=255) aaaaCTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCaa > 1:398428/1‑67 (MQ=255) aaaaCTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCaa > 1:824110/1‑67 (MQ=255) aaaaCTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCGTCGACCaa > 1:2112882/1‑67 (MQ=255) gCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCTCGGCCTTCGACCAACTGAGGATGGAATcc > 1:2431269/1‑68 (MQ=255) gCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGAATcc > 1:1632459/1‑68 (MQ=255) gCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGAATcc > 1:1988337/1‑68 (MQ=255) gCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGAATcc > 1:2415998/1‑68 (MQ=255) ccAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTg > 1:3118545/1‑55 (MQ=255) ccAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTg > 1:1343376/1‑55 (MQ=255) ccAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGAATCCGc > 1:1595181/1‑69 (MQ=255) ccAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGAATCCGc > 1:1434185/1‑69 (MQ=255) ccAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGAATCCGc > 1:1221459/1‑69 (MQ=255) aaTCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGAATCCGCAat < 1:2624463/69‑1 (MQ=255) aaTCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGAATCCGCAat < 1:2141002/69‑1 (MQ=255) aTCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGAATCCGCAata < 1:2516458/69‑1 (MQ=255) tCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGAATCCGCAatat > 1:1427505/1‑69 (MQ=255) tCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGAATCCGCAatat > 1:2206243/1‑69 (MQ=255) tCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGAATCCGCAatat > 1:287992/1‑69 (MQ=255) tCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGAATCCGCAatat > 1:464378/1‑69 (MQ=255) cTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGa > 1:1168806/1‑57 (MQ=255) cTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGAATCCGCAatat > 1:220520/1‑68 (MQ=255) cTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGAATCCGCAata > 1:930069/1‑67 (MQ=255) cgcgTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGAATCCGCAATATTCCAGCGcc < 1:1361208/69‑1 (MQ=255) cgcgTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGAATCCGCAATATTCCAGCGcc < 1:1214349/69‑1 (MQ=255) | ATGTATTCTCCTGATGGATGGTTCGGGTGAGGTAAAACTCGGCCAGTGCCAAATCTTCCGGGCGCGTGACTTTAATGTTATCCGCACGGCCTTCGACCAACTGAGGATGGAATCCGCAATATTCCAGCGCC > minE/1841447‑1841577 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |