| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,844,797 | G→A | 59.8% | D96D (GAC→GAT) | cysN ← | sulfate adenylyltransferase, subunit 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,844,797 | 0 | G | A | 59.8% | 31.7 / 57.7 | 82 | D96D (GAC→GAT) | cysN | sulfate adenylyltransferase, subunit 1 |
| Reads supporting (aligned to +/- strand): ref base G (16/17); new base A (35/14); total (51/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.06e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
TCGTGCCCTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGAGCC > minE/1844732‑1844851 | tCGTGCCCTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATc > 1:2291777/1‑68 (MQ=255)tCGTGCCCTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATc > 1:2037072/1‑68 (MQ=255)tCGTGCCCTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATc > 1:285476/1‑68 (MQ=255)tCGTGCCCTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATc > 1:2033244/1‑68 (MQ=255)tCGTGCCCTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATc > 1:2012579/1‑68 (MQ=255)tCGTGCCCTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATc > 1:2974800/1‑68 (MQ=255)tCGTGCCCTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATc > 1:2570858/1‑68 (MQ=255)tCGTGCCCTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATc > 1:2480871/1‑68 (MQ=255)tCGTGCCCTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATc > 1:666738/1‑68 (MQ=255)tCGTGCCCTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATc > 1:2971518/1‑68 (MQ=255)tCGTGCCCTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATc > 1:1180208/1‑68 (MQ=255)tCGTGCCCTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATc > 1:726758/1‑68 (MQ=255)tCGTGCCCTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATc > 1:2335718/1‑68 (MQ=255)tCGTGCCCTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATc > 1:2377717/1‑68 (MQ=255)tCGTGCCCTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATc > 1:878152/1‑68 (MQ=255) cGTGCCCTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCaa > 1:2865178/1‑69 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATc > 1:2308181/1‑63 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAAtag > 1:1041629/1‑66 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGt > 1:2887897/1‑69 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGt > 1:2471966/1‑69 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGt > 1:27478/1‑69 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGt > 1:2118874/1‑69 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGt > 1:2169801/1‑69 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGt > 1:2671011/1‑69 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGt > 1:2302887/1‑69 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGt > 1:242076/1‑69 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGt > 1:2922765/1‑69 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGt > 1:1006267/1‑69 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGt > 1:1113933/1‑69 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGt > 1:851247/1‑69 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGt > 1:381834/1‑69 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGt > 1:1555895/1‑69 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGt > 1:1547381/1‑69 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGt > 1:1194362/1‑69 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGt > 1:1464000/1‑69 (MQ=255) cccTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGt > 1:690198/1‑69 (MQ=255) ggCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCAATGGTGATGCCCTGTTc > 1:165683/1‑69 (MQ=255) ggCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCAATGGTGATGCCCTGTTc > 1:531122/1‑69 (MQ=255) aataaATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCAATTGt > 1:2238042/1‑51 (MQ=255) aataaATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCAATGGt > 1:3156448/1‑51 (MQ=255) aataaATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCAATGGt > 1:410587/1‑51 (MQ=255) aataaATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCAATGGt > 1:353037/1‑51 (MQ=255) aataaATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCAATGGt > 1:3239518/1‑51 (MQ=255) aaaTTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCaa < 1:72675/44‑1 (MQ=255) aaaTTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCaa < 1:3194934/44‑1 (MQ=255) aaaTTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCaa < 1:1019482/44‑1 (MQ=255) aaaTTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCaa < 1:1661965/44‑1 (MQ=255) aaaTTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCaa < 1:2210678/44‑1 (MQ=255) aaaTTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCaa < 1:1712495/44‑1 (MQ=255) aaaTTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCaa < 1:1922536/44‑1 (MQ=255) aaaTTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCaa < 1:2061794/44‑1 (MQ=255) aaaTTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCaa < 1:1984771/44‑1 (MQ=255) tttACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTg < 1:2901993/69‑1 (MQ=255) ttACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTg < 1:989999/68‑1 (MQ=255) ttACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTg < 1:1070175/68‑1 (MQ=255) ttACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTg < 1:1524828/68‑1 (MQ=255) ttACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTg < 1:2109447/68‑1 (MQ=255) ttACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGTGATg < 1:1571744/48‑1 (MQ=39) ttACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGTGATg < 1:698926/48‑1 (MQ=39) ttACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGTGATg < 1:1321044/48‑1 (MQ=39) ttACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGTGATg < 1:739054/48‑1 (MQ=39) ttACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGTGATg < 1:65939/48‑1 (MQ=39) ttACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGTGATg < 1:2510057/48‑1 (MQ=39) ttACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGTGATg < 1:1164686/48‑1 (MQ=39) ttACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGTGATg < 1:3365525/48‑1 (MQ=39) ttACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGTGATg < 1:2549087/48‑1 (MQ=39) ttACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGTGATg < 1:1865437/48‑1 (MQ=39) ttACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGTGATg < 1:3123852/48‑1 (MQ=39) ttACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGTGATg < 1:3062076/48‑1 (MQ=39) ttACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACATCAATAGTGATg < 1:2025240/48‑1 (MQ=39) ttACGCTTCTAGGTAGAGAAATAGCGGTAGGCCACATCAATAGTGATg < 1:1560726/48‑1 (MQ=255) aCGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTg < 1:398088/66‑1 (MQ=255) tAGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGAGcc < 1:1221712/69‑1 (MQ=255) aGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCaga > 1:2250083/1‑65 (MQ=255) aGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCaga > 1:2482173/1‑65 (MQ=255) aGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCaga > 1:950700/1‑65 (MQ=255) aGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCaga > 1:1178773/1‑65 (MQ=255) aGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCaga > 1:1369663/1‑65 (MQ=255) aGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCaga > 1:2194153/1‑65 (MQ=255) aGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCaga > 1:1874058/1‑65 (MQ=255) aGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCaga > 1:1874136/1‑65 (MQ=255) cGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTg < 1:1537350/44‑1 (MQ=255) | TCGTGCCCTGGGGTGTCGGCGATAATAAATTTACGCTTCTCGGTAGAGAAATAGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGAGCC > minE/1844732‑1844851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |