| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,844,848 | A→T | 38.0% | A79A (GCT→GCA) | cysN ← | sulfate adenylyltransferase, subunit 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,844,848 | 0 | A | T | 38.0% | 35.6 / 51.3 | 61 | A79A (GCT→GCA) | cysN | sulfate adenylyltransferase, subunit 1 |
| Reads supporting (aligned to +/- strand): ref base A (21/16); new base T (9/14); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.44e-01 | |||||||||||
TAGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCTTACTGTCGTTATGCAGCGATGAGAGCTG > minE/1844783‑1844913 | tAGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGAGcc < 1:1221712/69‑1 (MQ=255) aGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCaga > 1:950700/1‑65 (MQ=255) aGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCaga > 1:1178773/1‑65 (MQ=255) aGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCaga > 1:1369663/1‑65 (MQ=255) aGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCaga > 1:2482173/1‑65 (MQ=255) aGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCaga > 1:2250083/1‑65 (MQ=255) aGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCaga > 1:2194153/1‑65 (MQ=255) aGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCaga > 1:1874136/1‑65 (MQ=255) aGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCaga > 1:1874058/1‑65 (MQ=255) catCAATAGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCtttt > 1:1174150/3‑69 (MQ=255) catCAATAGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCtttt > 1:906691/3‑69 (MQ=255) catCAATAGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCtttt > 1:5442/3‑69 (MQ=255) catCAATAGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCtttt > 1:2955002/3‑69 (MQ=255) catCAATAGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCtttt > 1:2761820/3‑69 (MQ=255) catCAATAGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCtttt > 1:2063146/3‑69 (MQ=255) catCAATAGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCtttt > 1:1835966/3‑69 (MQ=255) catCAATAGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCttt > 1:3222408/3‑68 (MQ=255) catCAATAGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCttt > 1:1212396/3‑68 (MQ=255) tGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCGCCCTGGGTGc > 1:247796/1‑69 (MQ=255) tGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCGCCCTGGGTGc > 1:79926/1‑69 (MQ=255) tGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGa > 1:803481/1‑69 (MQ=255) tGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGa > 1:2349684/1‑69 (MQ=255) tGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGa > 1:2709132/1‑69 (MQ=255) tGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGa > 1:791345/1‑69 (MQ=255) tGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGa > 1:3336765/1‑69 (MQ=255) tGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTAGCCCTGGGTGCCGTGa > 1:2459123/1‑69 (MQ=255) tGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCAAGCTTTTCGCCCTGGGTGCCGTGa > 1:637024/1‑69 (MQ=255) cgcgCTCAGCTTGCAGGCTGTCCACCAGCAGTGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCt < 1:1282454/69‑1 (MQ=255) cgcgCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCt < 1:1830639/69‑1 (MQ=255) cgcgCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCt < 1:2724333/69‑1 (MQ=255) cgcgCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCt < 1:3244068/69‑1 (MQ=255) cgcgCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCt < 1:3232152/69‑1 (MQ=255) cgcgCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCt < 1:3050109/69‑1 (MQ=255) cgcgCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCt < 1:2397155/69‑1 (MQ=255) cgcgCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCt < 1:1569383/69‑1 (MQ=255) cgcgCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCt < 1:1616205/69‑1 (MQ=255) cgcgCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCt < 1:748010/69‑1 (MQ=255) cgcgCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCt < 1:1111117/69‑1 (MQ=255) cgcgCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCt < 1:2011180/69‑1 (MQ=255) cgcgCTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCt < 1:1724171/69‑1 (MQ=255) cgcgCTCAGCTTGCAGGCCGTCCACCAGCAGCGCCAGATCCAGCTTTTCGCGCTGGGTGCCGTGACGCt > 1:3211313/1‑69 (MQ=255) cgcgCTCAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCt > 1:1393266/1‑69 (MQ=255) cgcgCTCAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCt > 1:1940645/1‑69 (MQ=255) cTCAGCTTGCAGGCCGTCCACCAGCAGTGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCt < 1:46085/65‑1 (MQ=255) cAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCg > 1:2623163/1‑44 (MQ=255) cAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCGc > 1:862251/1‑45 (MQ=255) cAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCGc > 1:1175534/1‑45 (MQ=255) cAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCGc > 1:975061/1‑45 (MQ=255) cAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCGc > 1:3065283/1‑45 (MQ=255) cAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCGc > 1:296034/1‑45 (MQ=255) cAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCGc > 1:2767874/1‑45 (MQ=255) cAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCGc > 1:1336835/1‑45 (MQ=255) cAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCGc > 1:2158239/1‑45 (MQ=255) cAGCTTGCAGGCCGTCCACCAGAAGAGCCAGATCCAGCtttt > 1:2733041/1‑42 (MQ=255) gcagAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCTTACTGTCGTTATGCAGCGATGAGAGCt < 1:3322669/69‑1 (MQ=255) gcagAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCTTACTGTCGTTATGCAGCGATGAGAGCt < 1:3169178/69‑1 (MQ=255) gcagAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCTTACTGTCGTTATGCAGCGATGAGAGCt < 1:2617374/69‑1 (MQ=255) gcagAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCTTACTGTCGTTATGCAGCGATGAGAGCt < 1:2035362/69‑1 (MQ=255) gcagAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCTTACTGTCGTTATGCAGCGATGAGAGCt < 1:2174954/69‑1 (MQ=255) gcagAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCTTACTGTCGTTATGCAGCGATGAGAGCt < 1:892319/69‑1 (MQ=255) gcagAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCTTACTGTCGTTATGCAGCGATGAGAGCt < 1:80657/69‑1 (MQ=255) cagAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCTTACTGTCGTTATGCAGCGATGAGAGCt < 1:2978651/68‑1 (MQ=255) cagAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCTTACTGTCGTTATGCAGCGATGAGAGCTg < 1:179311/69‑1 (MQ=255) cagAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCTTACTGTCGTTATGCAGCGATGAGAGCTg < 1:20583/69‑1 (MQ=255) cagAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCTTACTGTCGTTATGCAGCGATGAGAGCTg < 1:1630359/69‑1 (MQ=255) cagAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCTTACTGTCGTTATGCAGCGATGAGAGCTg < 1:2141874/69‑1 (MQ=255) cagAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCTTACTGTCGTTATGCAGCGATGAGAGCTg < 1:2172865/69‑1 (MQ=255) cagAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCTTACTGTCGTTATGCAGCGATGAGAGCTg < 1:667541/69‑1 (MQ=255) cagAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCTTACTGTCGTTATGCAGCGATGAGAGCTg < 1:520810/69‑1 (MQ=255) | TAGCGGTAGGCCACGTCAATGGTGATGCCCTGTTCGCGCTCAGCTTGCAGGCCGTCCACCAGCAGAGCCAGATCCAGCTTTTCGCCCTGGGTGCCGTGACGCTTACTGTCGTTATGCAGCGATGAGAGCTG > minE/1844783‑1844913 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |