| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 21,538 | T→C | 34.7% | L490L (CTT→CTC) | ileS → | isoleucyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 21,538 | 0 | T | C | 34.7% | 100.1 / 83.9 | 141 | L490L (CTT→CTC) | ileS | isoleucyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (43/49); new base C (11/38); total (54/87) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.13e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
Only 100 of 141 total aligned reads displayed.
CACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTC > minE/21472‑21601 | cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt < 1:1034988/69‑1 (MQ=255) cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt < 1:3254784/69‑1 (MQ=255) cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt < 1:2287326/69‑1 (MQ=255) cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt < 1:1302675/69‑1 (MQ=255) cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt < 1:3168679/69‑1 (MQ=255) cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt < 1:2910705/69‑1 (MQ=255) cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt < 1:459185/69‑1 (MQ=255) cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt < 1:1914394/69‑1 (MQ=255) cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt < 1:2948627/69‑1 (MQ=255) cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt < 1:1672705/69‑1 (MQ=255) tGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTTCATCCGCGTACCCTTGAACTg < 1:2546387/69‑1 (MQ=255) tgtACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTg > 1:628209/1‑64 (MQ=255) tgtACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGAt > 1:2726320/1‑66 (MQ=255) tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaag < 1:2264271/69‑1 (MQ=255) tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaag < 1:247922/69‑1 (MQ=255) tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGga > 1:651498/1‑67 (MQ=255) tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGga > 1:3314107/1‑67 (MQ=255) tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGga > 1:2410449/1‑67 (MQ=255) tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGga > 1:2048193/1‑67 (MQ=255) tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGga > 1:767999/1‑67 (MQ=255) aCCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaag < 1:500221/68‑1 (MQ=255) aCCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaag < 1:1378824/68‑1 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:2534389/1‑69 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:919145/1‑69 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:2847012/1‑69 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:3197474/1‑69 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:1860364/1‑69 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:3089427/1‑69 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:3069172/1‑69 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:1415621/1‑69 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:723023/1‑69 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:2494909/1‑69 (MQ=255) cACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGc > 1:2630671/1‑67 (MQ=255) cACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCaa > 1:2730249/1‑69 (MQ=255) cACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCaa > 1:1306761/1‑69 (MQ=255) cACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCaa > 1:618026/1‑69 (MQ=255) aCTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCaaa < 1:2205541/69‑1 (MQ=255) aCTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCaaa < 1:2338457/69‑1 (MQ=255) aCTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCaaa < 1:1049683/69‑1 (MQ=255) aCTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCaaa < 1:1826942/69‑1 (MQ=255) aCTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCaaa < 1:259517/69‑1 (MQ=255) aCTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCaaa < 1:2413054/69‑1 (MQ=255) aCTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCaaa < 1:139521/69‑1 (MQ=255) aCTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCaaa < 1:727758/69‑1 (MQ=255) gTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAAcg > 1:305712/1‑69 (MQ=255) ttCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCGAAAcgc > 1:1811084/1‑69 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:3253275/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:694268/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:586166/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:5036/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:2948022/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:796001/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:3425864/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:833902/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:3282154/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:979049/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:1309676/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:2072118/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:1423768/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:2229038/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:2278536/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:1234253/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:1470631/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:1689633/51‑1 (MQ=255) tCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:3103448/50‑1 (MQ=255) cGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAAcgcg > 1:1142295/1‑68 (MQ=255) cGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAAcgcg > 1:3391175/1‑68 (MQ=255) cGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAAcgcg > 1:183494/1‑68 (MQ=255) tGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGtt < 1:2097905/68‑1 (MQ=255) tGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGtt < 1:3417387/68‑1 (MQ=255) tGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGtt < 1:1447911/68‑1 (MQ=255) tGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGtt < 1:580221/68‑1 (MQ=255) tGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGtt < 1:1541474/68‑1 (MQ=255) tGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGtt < 1:2374859/68‑1 (MQ=255) tGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGtt < 1:2597201/68‑1 (MQ=255) acaAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:2546756/44‑1 (MQ=255) acaAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:2621284/44‑1 (MQ=255) caAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGtt < 1:2526980/64‑1 (MQ=255) aaaGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTc < 1:1441852/69‑1 (MQ=255) aaaGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTc < 1:3196549/69‑1 (MQ=255) aaaGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTc < 1:760468/69‑1 (MQ=255) aaaGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTc < 1:2905767/69‑1 (MQ=255) aaGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTc < 1:1679322/68‑1 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCTTTGAAGTTGa > 1:551225/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGttg > 1:1491624/1‑66 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:2053686/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:665643/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:2645525/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:3340560/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:2378297/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:2455100/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:2957925/1‑69 (MQ=255) aGACACAGAAGAACTGAATCCGCGTAATCTCGAACTGATGGAAGAAGTTGCAAAAcg > 1:438195/1‑57 (MQ=255) tGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCgt < 1:3365331/69‑1 (MQ=255) cgcgTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCa > 1:199037/1‑58 (MQ=255) cgcgTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGtggtgg > 1:332539/1‑68 (MQ=255) cgcgTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGtggtgg > 1:2382572/1‑68 (MQ=255) cgcgTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTggg > 1:1371620/1‑69 (MQ=255) cgcgTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTggg < 1:1003257/69‑1 (MQ=255) aCCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGAtctc < 1:886584/69‑1 (MQ=255) | CACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTC > minE/21472‑21601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |