| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,858,220 | A→G | 14.1% | F454F (TTT→TTC) | cysI ← | sulfite reductase, beta subunit, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,858,220 | 0 | A | G | 14.1% | 170.0 / 14.7 | 98 | F454F (TTT→TTC) | cysI | sulfite reductase, beta subunit, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (68/16); new base G (8/6); total (76/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.81e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
ACACGCATCACGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCGGGAATGACACGCAAGCCA > minE/1858155‑1858287 | acacGCATCACGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATaaaa > 1:1022499/1‑69 (MQ=255)acacGCATCACGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATaaaa > 1:2898307/1‑69 (MQ=255) cATCACGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGAc > 1:969183/1‑67 (MQ=255) cATCACGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGAc > 1:655431/1‑67 (MQ=255) cATCACGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGAc > 1:436653/1‑67 (MQ=255) cATCACGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGAc > 1:3106872/1‑67 (MQ=255) cATCACGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGAc > 1:1013922/1‑67 (MQ=255) cATCACGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGAc > 1:1529955/1‑67 (MQ=255) cATCACGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGAc > 1:1267921/1‑67 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATa > 1:2062211/1‑57 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCa > 1:2640986/1‑67 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:2358620/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:1557832/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:169640/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:1715641/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:1794595/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:1803687/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:187219/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:1993056/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:2220454/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:1164236/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:2235887/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:2588363/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:308484/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:3103606/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:1086609/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:3397217/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:382739/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:594556/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:1018447/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:1321720/1‑68 (MQ=255) aCGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAg > 1:1222489/1‑68 (MQ=255) atGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATGAAAGACGGCAGGAAAc > 1:2384524/1‑68 (MQ=255) atGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATGAAAGACGGCAGGAAAc > 1:489993/1‑68 (MQ=255) atGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATGAAAGACGGCAGGAAACg > 1:2307240/1‑69 (MQ=255) atGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATGAAAGACGGCAGGAAACg > 1:643944/1‑69 (MQ=255) atGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATGAAAGACGGCAGGAAACg > 1:638083/1‑69 (MQ=255) atGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATGAAAGACGGCAGGAAACg > 1:1312496/1‑69 (MQ=255) atGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATGAAAGACGGCAGGAAACg > 1:622181/1‑69 (MQ=255) atGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATGAAAGACGGCAGGAAACg > 1:1064851/1‑69 (MQ=255) cTCATCGCTGCCACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGctc > 1:1731873/1‑69 (MQ=255) cTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGctc > 1:1392550/1‑69 (MQ=255) cTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGctc > 1:493213/1‑69 (MQ=255) cTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGctc > 1:893251/1‑69 (MQ=255) cTCATCGCTGACACCATGTTTCGCCATTAAATTACCGATGTTGTCGATAAAAGACGGCAGGAAACGctc > 1:901196/1‑69 (MQ=255) cGCTGTCACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCt < 1:1186873/68‑1 (MQ=255) cGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCt < 1:138217/68‑1 (MQ=255) cGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCt < 1:2406365/68‑1 (MQ=255) cGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCt < 1:1288389/68‑1 (MQ=255) gCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACgg > 1:2970265/1‑51 (MQ=255) gCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGc > 1:3116001/1‑52 (MQ=255) gCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGc > 1:2226455/1‑52 (MQ=255) gCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTc > 1:954613/1‑69 (MQ=255) gCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTc > 1:2551486/1‑69 (MQ=255) gCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTc > 1:2923914/1‑69 (MQ=255) gCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTc > 1:2477825/1‑69 (MQ=255) gCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTc > 1:2807300/1‑69 (MQ=255) gCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTc > 1:3340825/1‑69 (MQ=255) gCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTc > 1:1908716/1‑69 (MQ=255) gCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTc > 1:1644307/1‑69 (MQ=255) aTGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATc > 1:3060024/1‑67 (MQ=255) aTGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATc > 1:467131/1‑67 (MQ=255) aTGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATc > 1:1940895/1‑67 (MQ=255) aTGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATc > 1:1483430/1‑67 (MQ=255) aTGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATc > 1:1523950/1‑67 (MQ=255) aTTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCaa < 1:1150830/69‑1 (MQ=255) aTTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCaa < 1:1547264/69‑1 (MQ=255) aTTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCaa < 1:2789868/69‑1 (MQ=255) ttAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCaa < 1:1201493/68‑1 (MQ=255) ttAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCaa < 1:1168807/68‑1 (MQ=255) ttAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCaa < 1:1613866/68‑1 (MQ=255) ttAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCaa < 1:2357077/68‑1 (MQ=255) aaaTTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTc > 1:2699017/1‑69 (MQ=255) aaaTTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTc > 1:53843/1‑69 (MQ=255) aaaTTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTc > 1:1882097/1‑69 (MQ=255) aaaTTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTc > 1:879571/1‑69 (MQ=255) aaaTTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTc > 1:1209628/1‑69 (MQ=255) aaaTTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTc > 1:1110587/1‑69 (MQ=255) aaaTTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTc > 1:3025582/1‑69 (MQ=255) ttATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCggg < 1:1225488/69‑1 (MQ=255) ttATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCggg < 1:110167/69‑1 (MQ=255) ttATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCggg < 1:2160916/69‑1 (MQ=255) ttATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCggg < 1:1019604/69‑1 (MQ=255) aTCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCGGGa > 1:2052327/1‑68 (MQ=255) gATGTTGTCGATGAAAGACGTCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCGGGAATGa < 1:1340396/69‑1 (MQ=255) gATGTTGTCGATGAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCGGGAATGa < 1:309711/69‑1 (MQ=255) gATGTTGTCGATGAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCGGGAATGa < 1:155428/69‑1 (MQ=255) gATGTTGTCGATGAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCGGGAATGa < 1:1629765/69‑1 (MQ=255) gATGTTGTCGATGAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCGGGAATGa < 1:2034935/69‑1 (MQ=255) gATGTTGTCGATGAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCGGGAATGa < 1:1454831/69‑1 (MQ=255) ttgtCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCggg < 1:2901769/60‑1 (MQ=255) tAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCTAGTCGGGAATGACACGCAAGcc > 1:2490360/1‑68 (MQ=255) tAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCGGGAATGACACGCa > 1:2900433/1‑64 (MQ=255) tAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCGGGAATGACACGCAAGCCa > 1:1719343/1‑69 (MQ=255) tAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCGGGAATGACACGCAAGCCa > 1:1714941/1‑69 (MQ=255) tAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCGGGAATGACACGCAAGCCa > 1:1137864/1‑69 (MQ=255) tAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCGGGAATGACACGCAAGCCa > 1:1733425/1‑69 (MQ=255) tAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCGGGAATGACACGCAAGCCa > 1:477983/1‑69 (MQ=255) tAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCGGGAATGACACGCAAGCCa > 1:2196522/1‑69 (MQ=255) tAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCGGGAATGACACGCAAGCCa > 1:1372485/1‑69 (MQ=255) | ACACGCATCACGATATGCTCATCGCTGACACCATGTTTCGCCATTAAATTATCGATGTTGTCGATAAAAGACGGCAGGAAACGCTCTGCTTCCGCCATCGCCAGCGGGCAAGTCGGGAATGACACGCAAGCCA > minE/1858155‑1858287 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |