| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,858,376 | A→T | 24.7% | G402G (GGT→GGA) | cysI ← | sulfite reductase, beta subunit, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,858,376 | 0 | A | T | 24.7% | 145.6 / 46.9 | 93 | G402G (GGT→GGA) | cysI | sulfite reductase, beta subunit, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (35/35); new base T (18/5); total (53/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.77e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCATTCATTAACCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGTAATGCGGAAATCGCCTTTGTGGATCTT > minE/1858313‑1858432 | ggCATTCATTAACCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGc > 1:173308/1‑69 (MQ=255)ggCATTCATTAACCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGc < 1:2482406/69‑1 (MQ=255)ggCATTCATTAACCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGc < 1:1540381/69‑1 (MQ=255)ggCATTCATTAACCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGc < 1:1531970/69‑1 (MQ=255)ggCATTCATTAACCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGc < 1:364255/69‑1 (MQ=255)ggCATTCATTAACCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGc < 1:2825282/69‑1 (MQ=255)ggCATTCATTAACCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGc < 1:456912/69‑1 (MQ=255) gCATTCATTAACCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGc < 1:2183075/68‑1 (MQ=255) attcattAACCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCgat > 1:2713394/1‑69 (MQ=255) attcattAACCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCgat > 1:2030295/1‑69 (MQ=255) cattAACCCGCTCTCTTTGGCTATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCgatg > 1:329765/1‑67 (MQ=255) cattAACCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCgatg > 1:390553/1‑67 (MQ=255) cattAACCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCgatg > 1:2083996/1‑67 (MQ=255) cattAACCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCgatg > 1:1905422/1‑67 (MQ=255) cattAACCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCgatg > 1:2317484/1‑67 (MQ=255) cattAACCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCgatg > 1:519828/1‑67 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCgatgat > 1:1167329/1‑65 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCgatgat > 1:171466/1‑65 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCgatgat > 1:1670622/1‑65 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCgatgat > 1:158950/1‑65 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCgatgat > 1:338225/1‑65 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCgatgat > 1:905903/1‑65 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCgatgat > 1:1532232/1‑65 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCgatgat > 1:1485105/1‑65 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCgatgat > 1:81136/1‑65 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCgat > 1:871216/1‑62 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGa < 1:220515/69‑1 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGa > 1:461433/1‑69 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGa > 1:2543748/1‑69 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGa < 1:565222/69‑1 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGa < 1:723433/69‑1 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGa < 1:1037969/69‑1 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGa < 1:966399/69‑1 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGa > 1:1167940/1‑69 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGa < 1:1209235/69‑1 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGa < 1:796760/69‑1 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGa > 1:1343071/1‑69 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGa > 1:2637952/1‑69 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGa < 1:2848806/69‑1 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGa < 1:3201970/69‑1 (MQ=255) aaCCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGa < 1:1492565/69‑1 (MQ=255) tGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGATTCTGGTTCGc < 1:3195613/67‑1 (MQ=255) gCGATCTTGTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt < 1:1495303/68‑1 (MQ=255) gCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGa < 1:2004126/54‑1 (MQ=255) gCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGATTCTGGTTCGCCg < 1:2000730/67‑1 (MQ=255) gCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt < 1:2556841/68‑1 (MQ=255) gCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt < 1:2926435/68‑1 (MQ=255) gCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt < 1:1881407/68‑1 (MQ=255) gCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt < 1:1430509/68‑1 (MQ=255) gCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGCACACCGGCGATGATCAGATTCTGGTTCGCCGt < 1:457201/68‑1 (MQ=255) tcttctCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt < 1:786386/64‑1 (MQ=255) tcttctCGATCTTCGCTTTCTCGCTTTCCGGCACTCCGGCGATGATCAGATTc > 1:1802188/1‑53 (MQ=255) tcttctCGATCTTCGCTTTCTCGCTTTCCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATg > 1:3366582/1‑68 (MQ=255) tcttctCGATCTTCGCTTTCTCGCTTTCCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATg > 1:988154/1‑68 (MQ=255) tcttctCGATCTTCGCTTTCTCGCTTTCCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATg > 1:567480/1‑68 (MQ=255) tcttctCGATCTTCGCTTTCTCGCTTTCCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATg > 1:3214832/1‑68 (MQ=255) tcttctCGATCTTCGCTTTCTCGCTTTCCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATg > 1:569793/1‑68 (MQ=255) tcttctCGATCTTCGCTTTCTCGCTTTCCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATg > 1:699227/1‑68 (MQ=255) tcttctCGATCTTCGCTTTCTCGCTTTCCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATg > 1:979240/1‑68 (MQ=255) tcttctCGATCTTCGCTTTCTCGCTTTCCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATg > 1:3137671/1‑68 (MQ=255) tcttctCGATCTTCGCTTTCTCGCTTTCCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATg > 1:2069225/1‑68 (MQ=255) tcttctCGATCTTCGCTTTCTCGCTTTCCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATg > 1:1102506/1‑68 (MQ=255) tcttctCGATCTTCGCTTTCTCGCTTTCCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATg > 1:1154398/1‑68 (MQ=255) tcttctCGATCTTCGCTTTCTCGCTTTCCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATg > 1:2756697/1‑68 (MQ=255) tcttctCGATCTTCGCTTTCTCGCTTTCCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATg > 1:2157302/1‑68 (MQ=255) tcttctCGATCTTCGCTTTCTCGCTTTCCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATg > 1:131053/1‑68 (MQ=255) tcttctCGATCTTCGCTTTCTCGCTTTCCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATg > 1:2569412/1‑68 (MQ=255) tcttctCGATCTTCGCTTTCTCGCTTTCCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATg > 1:1386006/1‑68 (MQ=255) tcttctCGATCTTCGCTTTCTCGCTTTCCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATg > 1:1553003/1‑68 (MQ=255) cgctttcttgctttcCGGTACACCGTCGATGATCAGATTCTGGTTCGCCGt < 1:2452664/51‑1 (MQ=39) cgctttctggctttcCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt > 1:2013790/1‑51 (MQ=255) cgctttctcgctttcCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt < 1:1414499/51‑1 (MQ=255) cgctttctcgctttcCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt > 1:1443016/1‑51 (MQ=255) cgctttctcgctttcCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt < 1:710493/51‑1 (MQ=255) cgctttctcgctttcCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt > 1:1254684/1‑51 (MQ=255) cgctttctcgctttcCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt > 1:1079050/1‑51 (MQ=255) cgctttctcgctttcCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt > 1:3071614/1‑51 (MQ=255) cgctttctcgctttcCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt > 1:1584710/1‑51 (MQ=255) cgctttctcgctttcCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt > 1:2187576/1‑51 (MQ=255) cgctttctcgctttcCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt > 1:2240227/1‑51 (MQ=255) cgctttctcgctttcCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt < 1:3283095/51‑1 (MQ=255) cgctttctcgctttcCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt < 1:2819092/51‑1 (MQ=255) cgctttctcgctttcCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt > 1:2865766/1‑51 (MQ=255) cgctttctcgctttcCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGt > 1:3118677/1‑51 (MQ=255) cgctttctcgctttcCGGTACACCGGCGATGATCAGATTCTGGATCGCCGt < 1:1265128/51‑1 (MQ=255) tttctcgctttcCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATGCGGAAATCACCTTtgt < 1:394661/68‑1 (MQ=255) tttctcgctttcCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATGCGGAAATCACCTTtgt < 1:2307096/68‑1 (MQ=255) tttctcgctttcCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATGCGGAAATCACCTTtgt < 1:356476/68‑1 (MQ=255) tttctcgctttcCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATGCGGAAATCACCTTtgt < 1:788597/68‑1 (MQ=255) tttctcgctttcCGGCACTCCGGCGATGATCAGATTCTGGTTCGCCGTAATGCGGAAATCACCTTtgt < 1:1056363/68‑1 (MQ=255) ttctcgctttcCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGTAATGCGGAAATCGCCTTtgtg > 1:2635344/1‑68 (MQ=255) tttcCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGTAATGCGGAAATCGCCTTTGTGGATCtt < 1:70372/67‑1 (MQ=255) tttcCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGTAATGCGGAAATCGCCTTTGTGGATCtt < 1:1250261/67‑1 (MQ=255) | GGCATTCATTAACCCGCTCTCTTTGGCGATCTTCTCGATCTTCGCTTTCTCGCTTTCCGGTACACCGGCGATGATCAGATTCTGGTTCGCCGTAATGCGGAAATCGCCTTTGTGGATCTT > minE/1858313‑1858432 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |