Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 139,698 C→T 17.6% V157I (GTA→ATA) ‡ htrE ← predicted outer membrane usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE139,6980CT17.6% 211.5 / 27.4 129V157I (GTA→ATA) ‡htrEpredicted outer membrane usher protein
Reads supporting (aligned to +/- strand):  ref base C (62/44);  new base T (10/13);  total (72/57)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

Only 100 of 129 total aligned reads displayed.

GCCGCATTAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGG  >  minE/139637‑139765
                                                             |                                                                   
gCCGCATTAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGc                                                               >  1:2432812/1‑68 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                        >  1:1180834/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                        >  1:2823959/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                        >  1:2946808/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                        >  1:2347335/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                        >  1:3298814/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                        >  1:1213071/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                        >  1:36701/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                        <  1:2070033/69‑1 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                        >  1:1624846/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                        >  1:1905314/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                        >  1:1677882/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                        >  1:1706373/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                        <  1:100206/69‑1 (MQ=255)
        aaTTCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                      >  1:2277998/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAg                                                                 >  1:1267584/1‑58 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTg                                                            >  1:2939106/1‑63 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGaa                                                       >  1:1844352/1‑68 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                      >  1:1364024/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                      >  1:2611467/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                      >  1:2443457/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                      >  1:3224452/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                      >  1:2285136/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                      >  1:126543/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                      >  1:62521/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                      >  1:1071774/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                      >  1:1627833/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                      >  1:1027762/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                      >  1:90514/1‑69 (MQ=255)
           gCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTCGAGGAACGTc                                                   <  1:1053444/69‑1 (MQ=255)
            ccGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTc                                                   >  1:933159/1‑68 (MQ=255)
                       tAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACg                                       <  1:2359837/69‑1 (MQ=255)
                       tAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACg                                       <  1:1644478/69‑1 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCa                                           >  1:1352630/1‑63 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                     >  1:1346478/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                     >  1:1603957/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                     <  1:2229606/69‑1 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                     >  1:2221525/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                     >  1:2900362/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                     >  1:2052282/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                     >  1:1340056/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                     >  1:1947245/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                     >  1:3056430/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                     >  1:3239482/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                     >  1:3416418/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                     >  1:871359/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGt                                      >  1:2103966/1‑68 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGt                                      >  1:1158495/1‑68 (MQ=255)
                         aCGATGGATAAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGt                                      >  1:2943867/1‑68 (MQ=255)
                           gATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                    <  1:1818288/68‑1 (MQ=255)
                           gATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                    <  1:2735954/68‑1 (MQ=255)
                           gATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                    <  1:2649183/68‑1 (MQ=255)
                           gATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                    <  1:1512616/68‑1 (MQ=255)
                           gATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                    <  1:1930711/68‑1 (MQ=255)
                            aTGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                    <  1:1819848/67‑1 (MQ=255)
                            aTGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                    <  1:1424009/67‑1 (MQ=255)
                              ggATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                    <  1:2964844/65‑1 (MQ=255)
                              ggATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                    <  1:661862/65‑1 (MQ=255)
                              ggATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                    <  1:3035118/65‑1 (MQ=255)
                              ggATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                    <  1:78405/65‑1 (MQ=255)
                              ggATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                    <  1:2216036/65‑1 (MQ=255)
                              ggATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                    <  1:60607/65‑1 (MQ=255)
                              ggATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                    <  1:2654917/65‑1 (MQ=255)
                              ggATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                    <  1:2245822/65‑1 (MQ=255)
                              ggATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                    <  1:3301159/65‑1 (MQ=255)
                                 tCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACg                                       >  1:1881248/1‑59 (MQ=255)
                                 tCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACg                                       >  1:1737039/1‑59 (MQ=255)
                                 tCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGtt                              <  1:2715569/68‑1 (MQ=255)
                                 tCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGtt                              <  1:78475/68‑1 (MQ=255)
                                 tCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGtt                              <  1:811234/68‑1 (MQ=255)
                                  cAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGtt                              <  1:1273878/67‑1 (MQ=255)
                                    aCATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                    <  1:1129147/59‑1 (MQ=255)
                                      aTAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACg                          >  1:756191/1‑67 (MQ=255)
                                        aGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTATCGTCAt                      <  1:442784/69‑1 (MQ=255)
                                        aGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCAt                      <  1:467084/69‑1 (MQ=255)
                                         gTTTTGATAATTCTTCATAATCCATGCCTGTGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                     <  1:749256/69‑1 (MQ=255)
                                         gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                     <  1:1607980/69‑1 (MQ=255)
                                         gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                     <  1:3185350/69‑1 (MQ=255)
                                         gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                     <  1:2716367/69‑1 (MQ=255)
                                         gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                     <  1:2508012/69‑1 (MQ=255)
                                         gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                     <  1:1444888/69‑1 (MQ=255)
                                         gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                     <  1:1542301/69‑1 (MQ=255)
                                         gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                     <  1:3377166/69‑1 (MQ=255)
                                         gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                     <  1:1022105/69‑1 (MQ=255)
                                         gTTTTGATAATTCATCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                     <  1:2322446/69‑1 (MQ=255)
                                          ttttGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                     <  1:2030272/68‑1 (MQ=255)
                                               atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                >  1:3187997/2‑68 (MQ=255)
                                               atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                >  1:3069268/2‑68 (MQ=255)
                                               atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                >  1:2063249/2‑68 (MQ=255)
                                               atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                >  1:2896663/2‑68 (MQ=255)
                                               atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                >  1:2141195/2‑68 (MQ=255)
                                               atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                >  1:2365083/2‑68 (MQ=255)
                                                    ttttCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGaa           <  1:2434839/68‑1 (MQ=255)
                                                    ttttCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGaa           <  1:1530033/68‑1 (MQ=255)
                                                            aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg  <  1:1713788/69‑1 (MQ=255)
                                                            aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg  <  1:1226880/69‑1 (MQ=255)
                                                            aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg  <  1:559956/69‑1 (MQ=255)
                                                            aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg  <  1:2028433/69‑1 (MQ=255)
                                                            aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg  <  1:717558/69‑1 (MQ=255)
                                                            aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg  <  1:1014071/69‑1 (MQ=255)
                                                             |                                                                   
GCCGCATTAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGG  >  minE/139637‑139765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: