| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,862,627 | T→C | 59.6% | R163R (CGT→CGC) | ygcN → | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,862,627 | 0 | T | C | 59.6% | 38.6 / 57.1 | 84 | R163R (CGT→CGC) | ygcN | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base T (6/28); new base C (11/39); total (17/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGA > minE/1862562‑1862695 | gACGATATTCTTCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCGGAACGCCAc > 1:1130323/1‑69 (MQ=255)gACGATATTCTTCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCGGAACGCCAc > 1:518745/1‑69 (MQ=255)gACGATATTCTTCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCGGAACGCCAc > 1:47596/1‑69 (MQ=255)gACGATATTCTTCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCGGAACGCCAc > 1:411797/1‑69 (MQ=255)gACGATATTCTTCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCGGAACGCCAc > 1:34689/1‑69 (MQ=255)gACGATATTCTTCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCGGAACGCCAc > 1:3180566/1‑69 (MQ=255)gACGATATTCTTCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCGGAACGCCAc > 1:300835/1‑69 (MQ=255)gACGATATTCTTCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCGGAACGCCAc > 1:2832311/1‑69 (MQ=255)gACGATATTCTTCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCGGAACGCCAc > 1:2025698/1‑69 (MQ=255)gACGATATTCTTCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCGGAACGCCAc > 1:1851630/1‑69 (MQ=255)gACGATATTCTTCGCGCCCGTTATGTGGTGCTGGCAGAAGGTCCCAACAGCGTCCTGGCGGAACGCCAc > 1:3306068/1‑69 (MQ=255) aCGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCAc > 1:2020098/1‑68 (MQ=255) aCGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACg > 1:1260418/1‑69 (MQ=255) tatTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTTCCAACAGCGTCCTGGCTGAACGTCACGGGtt < 1:2251109/69‑1 (MQ=255) tatTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGtt < 1:3185280/69‑1 (MQ=255) tatTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGtt < 1:3308624/69‑1 (MQ=255) tatTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGtt < 1:422081/69‑1 (MQ=255) tatTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGtt < 1:668484/69‑1 (MQ=255) tatTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGtt < 1:202399/69‑1 (MQ=255) tatTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGtt < 1:3227736/69‑1 (MQ=255) tatTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGtt < 1:890137/69‑1 (MQ=255) aTGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCctgctg > 1:3038520/1‑69 (MQ=255) gCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGc < 1:1938974/69‑1 (MQ=255) gCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGc < 1:1828195/69‑1 (MQ=255) gCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGc < 1:3212437/69‑1 (MQ=255) gCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGc < 1:2929580/69‑1 (MQ=255) gCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGc < 1:2161446/69‑1 (MQ=255) gCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGc < 1:2859072/69‑1 (MQ=255) gCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGc < 1:1126123/69‑1 (MQ=255) ggTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGaa > 1:1709442/1‑57 (MQ=255) ggTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTg > 1:2462037/1‑69 (MQ=255) ggTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTg > 1:943649/1‑69 (MQ=255) aCAGCGTCCTGGCGGAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGTATCa < 1:2622979/69‑1 (MQ=255) aCAGCGTCCTGGCGGAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCa < 1:1702242/69‑1 (MQ=255) aCAGCGTCCTGGCGGAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCa < 1:291265/69‑1 (MQ=255) aCAGCGTCCTGGCGGAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCa < 1:2717702/69‑1 (MQ=255) aCAGCGTCCTGGCGGAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCa < 1:1345056/69‑1 (MQ=255) aCAGCGTCCTGGCGGAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCa < 1:2685995/69‑1 (MQ=255) aCAGCGTCCTGGCGGAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCa < 1:1687294/69‑1 (MQ=255) aCAGCGTCCTGGCGGAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCa < 1:1560701/69‑1 (MQ=255) aCAGCGTCCTGGCGGAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCa < 1:3001737/69‑1 (MQ=255) aCAGCGTCCTGGCGGAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCa < 1:2485553/69‑1 (MQ=255) aCAGCGTCCTGGCGGAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCa < 1:1267467/69‑1 (MQ=255) aCAGCGTCCTGGCGGAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCa < 1:490025/69‑1 (MQ=255) aCAGCGTCCTGGCGGAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCa < 1:518251/69‑1 (MQ=255) aCAGCGTCCTGGCGGAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCa < 1:318690/69‑1 (MQ=255) aCAGCGTCCTGGCGGAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCa < 1:1784578/69‑1 (MQ=255) aCAGCGTCCTGGCGGAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCa < 1:78140/69‑1 (MQ=255) aCAGCGTCCTGGCGGAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCa < 1:328103/69‑1 (MQ=255) gTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGc < 1:392250/47‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:1227467/56‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:3329199/56‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:123904/56‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:1611257/56‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:648411/56‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:667835/56‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:690400/56‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:787222/56‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:874700/56‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:971396/56‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:300028/56‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:1815512/56‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:1995556/56‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:2102695/56‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:2129870/56‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:2142763/56‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:2178821/56‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:2240795/56‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:2515880/56‑1 (MQ=255) ggAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:286277/56‑1 (MQ=255) gAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:2432717/56‑1 (MQ=255) gAACGCCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCATTGGGGATCaa < 1:2935360/56‑1 (MQ=255) cGTCACGGGTTAGTGTCTCGTCCTGCTGTCGAAGc < 1:435709/35‑1 (MQ=255) cGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGc < 1:1584100/35‑1 (MQ=255) cGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGc < 1:175124/35‑1 (MQ=255) cGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGc < 1:1801561/35‑1 (MQ=255) cGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGc < 1:3307882/35‑1 (MQ=255) cGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGc < 1:3311925/35‑1 (MQ=255) cGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGc < 1:1560221/35‑1 (MQ=255) cGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGc < 1:1463777/35‑1 (MQ=255) cGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGc < 1:554842/35‑1 (MQ=255) cGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGc < 1:2548939/35‑1 (MQ=255) cGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGc < 1:2370858/35‑1 (MQ=255) cGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGc < 1:2429395/35‑1 (MQ=255) tCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGa < 1:3371881/69‑1 (MQ=255) tCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGa < 1:796410/69‑1 (MQ=255) | GACGATATTCTCCGCGCCCGTTATGTGGTGCTGGCAGAAGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGA > minE/1862562‑1862695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |