| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,864,396 | A→G | 53.5% | intergenic (+143/+5) | ygcP → / ← ygcQ | predicted anti‑terminator regulatory protein/predicted flavoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,864,396 | 0 | A | G | 53.5% | 14.7 / 84.0 | 101 | intergenic (+143/+5) | ygcP/ygcQ | predicted anti‑terminator regulatory protein/predicted flavoprotein |
| Reads supporting (aligned to +/- strand): ref base A (28/19); new base G (17/37); total (45/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
CTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAAC > minE/1864333‑1864463 | cTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGt < 1:2072008/69‑1 (MQ=255)cTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGt < 1:2395648/69‑1 (MQ=255)cTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGt < 1:2912711/69‑1 (MQ=255) tGACGCGTCTTATCAGGCCTACAAGGCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGt < 1:1526401/67‑1 (MQ=255) tCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGt < 1:201574/60‑1 (MQ=255) tCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCaa > 1:1119705/1‑69 (MQ=255) tCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCaa > 1:163106/1‑69 (MQ=255) tCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTCGCaa > 1:34184/1‑69 (MQ=255) gcttACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGTTATTGGCAATCTGCGTg < 1:3305050/65‑1 (MQ=39) gcttACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGTTATTGGCAATCTGCGTg < 1:3198353/65‑1 (MQ=39) gcttACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGTTATTGGCAATCTGCGTg < 1:3418769/65‑1 (MQ=39) gcttACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGTTATTGGCAATCTGCGTg < 1:408249/65‑1 (MQ=39) gcttACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGTTATTGGCAATCTGCGTg < 1:2295269/65‑1 (MQ=39) gcttACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGTTATTGGCAATCTGCGTg < 1:2224978/65‑1 (MQ=39) gcttACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGTTATTGGCAATCTGCGTg < 1:737936/65‑1 (MQ=39) gcttACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGTTATTGGCAATCTGCGTg < 1:1891487/65‑1 (MQ=39) gcttACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGTTATTGGCAATCTGCGTg < 1:168049/65‑1 (MQ=39) gcttACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGTTATTGGCAATCTGCGTg < 1:1663745/65‑1 (MQ=39) gcttACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGTTATTGGCAATCTGCGTg < 1:868925/65‑1 (MQ=39) gcttACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGTTATTGGCAATCTGCGTg < 1:1235142/65‑1 (MQ=39) gcttACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGTTATTGGCAATCTGCGTg < 1:1292005/65‑1 (MQ=39) gCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTgg > 1:2246534/1‑57 (MQ=255) gCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGa > 1:2907971/1‑69 (MQ=255) gCCTACAAGTCCCCAGGACAGAACCGTAGGCAGGATCATCCGTCAACACGTTATTGGCAATCTGCGTg > 1:2798431/1‑68 (MQ=39) tACAAGTCCCCCGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGt > 1:1240182/1‑48 (MQ=37) tACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAgcac > 1:2931851/1‑46 (MQ=37) tACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACg > 1:1863301/1‑47 (MQ=37) tACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACg > 1:2372804/1‑47 (MQ=37) tACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGt > 1:2404741/1‑48 (MQ=37) tACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGt > 1:1896610/1‑48 (MQ=37) tACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGt > 1:2043992/1‑48 (MQ=37) tACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGt > 1:136285/1‑48 (MQ=37) tACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGt > 1:368684/1‑48 (MQ=37) tACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGt > 1:3248181/1‑48 (MQ=37) tACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGt > 1:1059245/1‑48 (MQ=37) tACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGt > 1:1066549/1‑48 (MQ=37) tACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGt > 1:1880086/1‑48 (MQ=37) tACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGt > 1:1444037/1‑48 (MQ=37) tACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGt > 1:779225/1‑48 (MQ=37) tACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGt > 1:1020653/1‑48 (MQ=37) tACAAGTCCCCAGCCCAGAACCGTAGGCAGGATCATCCGGCAGCACGt > 1:1552910/1‑48 (MQ=37) cAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGtt > 1:2040633/1‑69 (MQ=255) cAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGtt > 1:2106305/1‑69 (MQ=255) cAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGtt > 1:120448/1‑69 (MQ=255) cAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGtt > 1:1213930/1‑69 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTc > 1:2583770/1‑67 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTc > 1:2680231/1‑67 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTc > 1:1268342/1‑67 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTc > 1:2872290/1‑67 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTc > 1:797418/1‑67 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTc > 1:3087446/1‑67 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTc > 1:1543623/1‑67 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTc > 1:1154854/1‑67 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTc > 1:819908/1‑67 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTc > 1:3248637/1‑67 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTc > 1:1752308/1‑67 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTc > 1:1869118/1‑67 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTc > 1:1222702/1‑67 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTc > 1:1208304/1‑67 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCAc < 1:2444988/69‑1 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCAc < 1:420090/69‑1 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCAc < 1:1320493/69‑1 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCAc < 1:3002172/69‑1 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCAc < 1:2453431/69‑1 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCAc < 1:3138813/69‑1 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCAc < 1:670544/69‑1 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCAc < 1:355455/69‑1 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCAc < 1:2040145/69‑1 (MQ=255) cccAGGCCAGAACCGTAGGCAGGATCATCAGGCAACAAGTTATTGGCAATCTGCGTGAATGTTTGTCAc < 1:1996002/69‑1 (MQ=255) ggCAGGATCATCCGGCAGCACGTTATTGGCAATCTGCGTg < 1:108476/40‑1 (MQ=255) atcatcCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCAc < 1:411184/46‑1 (MQ=255) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:40271/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:348437/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:1015649/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:642610/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:481892/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:636345/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:577430/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:2453896/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:141190/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:1336512/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:1549966/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:1586277/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:1768963/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:1797959/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:2290904/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:3393730/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:246156/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:2864809/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:301706/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:3154777/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:3250986/47‑1 (MQ=38) tcatcCGGCAGCACGTTATTGGCAATCTGCGTGAATGGTTGTCACCa < 1:3324237/47‑1 (MQ=38) catcCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGt > 1:733558/1‑41 (MQ=255) catcCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCa > 1:3211494/1‑68 (MQ=255) gCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGtcatc < 1:565027/68‑1 (MQ=255) gCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGtcatc < 1:1057203/68‑1 (MQ=255) gCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGtcatc < 1:1322834/68‑1 (MQ=255) aaCACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAAc < 1:2695859/69‑1 (MQ=255) aaCACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAAc < 1:792311/69‑1 (MQ=255) | CTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGTTATTGGCAATCTGCGTGAATGTTTGTCACCAGTGCCTCCAGCACCACTTTCCAGTCATCAAC > minE/1864333‑1864463 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |