Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,866,978	A→G	58.6%	Y125Y (TAT→TAC)	ygcS ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,866,978	0	A	G	58.6%	12.9 / 26.2	41	Y125Y (TAT→TAC)	ygcS	predicted transporter
Reads supporting (aligned to +/- strand):  ref base A (6/11);  new base G (15/9);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

TGCGCCCAGCAAAATACCGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCG  >  minE/1866912‑1867036                                                                   |                                                           tGCGCCCAGCAAAATACCGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACGTACTGaata                                                          <  1:36284/69‑1 (MQ=255) tGCGCCCAGCAAAATACCGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGaata                                                          <  1:2798831/69‑1 (MQ=255)     cccAGCAAAATACCGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATc                                                       <  1:853749/68‑1 (MQ=255)     cccAGCAAAATACCGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATc                                                       <  1:2811866/68‑1 (MQ=255)     cccAGCAAAATACCGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATc                                                       <  1:2638915/68‑1 (MQ=255)                aCCGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGAcc                                           <  1:948176/69‑1 (MQ=255)                 ccGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGAcc                                           <  1:826935/68‑1 (MQ=255)                 ccGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCa                                          >  1:3263341/1‑69 (MQ=255)                            gcgcGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCaa                               <  1:918306/69‑1 (MQ=255)                            gcgcGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCaa                               <  1:763999/69‑1 (MQ=255)                            gcgcGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCaa                               <  1:2628499/69‑1 (MQ=255)                                       aTTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCATAcc                                           <  1:2163560/46‑1 (MQ=255)                                             ccagcAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATaa              <  1:2087004/69‑1 (MQ=255)                                             ccagcAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATaa              <  1:2554001/69‑1 (MQ=255)                                                 cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                               <  1:3045009/48‑1 (MQ=255)                                                 cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                               <  1:3045742/48‑1 (MQ=255)                                                 cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                               <  1:127298/48‑1 (MQ=255)                                                 cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                               <  1:3333534/48‑1 (MQ=255)                                                 cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                               <  1:377890/48‑1 (MQ=255)                                                 cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                               <  1:969913/48‑1 (MQ=255)                                                 cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                               <  1:1219052/48‑1 (MQ=255)                                                 cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                               <  1:1946538/48‑1 (MQ=255)                                                 cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                               <  1:2401428/48‑1 (MQ=255)                                                      tgtgACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATAAGATGc         >  1:2759461/1‑65 (MQ=255)                                                      tgtgACCTACTGAATAATCGCCTCCCAGAACAATGCCAATCAAAATGCGCAGTCCAATAAGATGc         >  1:355379/1‑65 (MQ=255)                                                         gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:2047434/1‑69 (MQ=255)                                                         gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:1332504/1‑69 (MQ=255)                                                         gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:1629384/1‑69 (MQ=255)                                                         gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:1716159/1‑69 (MQ=255)                                                         gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:1886477/1‑69 (MQ=255)                                                         gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:736913/1‑69 (MQ=255)                                                         gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:624562/1‑69 (MQ=255)                                                         gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:398331/1‑69 (MQ=255)                                                         gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:3025677/1‑69 (MQ=255)                                                         gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:2685982/1‑69 (MQ=255)                                                         gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:3303100/1‑69 (MQ=255)                                                         gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:32124/1‑69 (MQ=255)                                                         gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:31245/1‑69 (MQ=255)                                                         gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:2929913/1‑69 (MQ=255)                                                         gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:3040807/1‑69 (MQ=255)                                                          aCCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCg  >  1:753435/1‑68 (MQ=255)                                                          aCCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCg  >  1:2920595/1‑68 (MQ=255)                                                          aCCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGACCAATAAGATGc         >  1:766893/1‑61 (MQ=255)                                                                   |                                                           TGCGCCCAGCAAAATACCGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCG  >  minE/1866912‑1867036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑