Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,866,978 A→G 58.6% Y125Y (TAT→TAC ygcS ← predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,866,9780AG58.6% 12.9 / 26.2 41Y125Y (TAT→TACygcSpredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (6/11);  new base G (15/9);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

TGCGCCCAGCAAAATACCGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCG  >  minE/1866912‑1867036
                                                                  |                                                          
tGCGCCCAGCAAAATACCGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACGTACTGaata                                                          <  1:36284/69‑1 (MQ=255)
tGCGCCCAGCAAAATACCGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGaata                                                          <  1:2798831/69‑1 (MQ=255)
    cccAGCAAAATACCGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATc                                                       <  1:853749/68‑1 (MQ=255)
    cccAGCAAAATACCGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATc                                                       <  1:2811866/68‑1 (MQ=255)
    cccAGCAAAATACCGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATc                                                       <  1:2638915/68‑1 (MQ=255)
               aCCGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGAcc                                           <  1:948176/69‑1 (MQ=255)
                ccGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGAcc                                           <  1:826935/68‑1 (MQ=255)
                ccGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCa                                          >  1:3263341/1‑69 (MQ=255)
                           gcgcGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCaa                               <  1:918306/69‑1 (MQ=255)
                           gcgcGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCaa                               <  1:763999/69‑1 (MQ=255)
                           gcgcGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCaa                               <  1:2628499/69‑1 (MQ=255)
                                      aTTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCATAcc                                           <  1:2163560/46‑1 (MQ=255)
                                            ccagcAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATaa              <  1:2087004/69‑1 (MQ=255)
                                            ccagcAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATaa              <  1:2554001/69‑1 (MQ=255)
                                                cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                               <  1:3045009/48‑1 (MQ=255)
                                                cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                               <  1:3045742/48‑1 (MQ=255)
                                                cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                               <  1:127298/48‑1 (MQ=255)
                                                cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                               <  1:3333534/48‑1 (MQ=255)
                                                cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                               <  1:377890/48‑1 (MQ=255)
                                                cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                               <  1:969913/48‑1 (MQ=255)
                                                cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                               <  1:1219052/48‑1 (MQ=255)
                                                cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                               <  1:1946538/48‑1 (MQ=255)
                                                cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                               <  1:2401428/48‑1 (MQ=255)
                                                     tgtgACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATAAGATGc         >  1:2759461/1‑65 (MQ=255)
                                                     tgtgACCTACTGAATAATCGCCTCCCAGAACAATGCCAATCAAAATGCGCAGTCCAATAAGATGc         >  1:355379/1‑65 (MQ=255)
                                                        gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:2047434/1‑69 (MQ=255)
                                                        gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:1332504/1‑69 (MQ=255)
                                                        gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:1629384/1‑69 (MQ=255)
                                                        gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:1716159/1‑69 (MQ=255)
                                                        gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:1886477/1‑69 (MQ=255)
                                                        gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:736913/1‑69 (MQ=255)
                                                        gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:624562/1‑69 (MQ=255)
                                                        gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:398331/1‑69 (MQ=255)
                                                        gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:3025677/1‑69 (MQ=255)
                                                        gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:2685982/1‑69 (MQ=255)
                                                        gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:3303100/1‑69 (MQ=255)
                                                        gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:32124/1‑69 (MQ=255)
                                                        gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:31245/1‑69 (MQ=255)
                                                        gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:2929913/1‑69 (MQ=255)
                                                        gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg  >  1:3040807/1‑69 (MQ=255)
                                                         aCCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCg  >  1:753435/1‑68 (MQ=255)
                                                         aCCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCg  >  1:2920595/1‑68 (MQ=255)
                                                         aCCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGACCAATAAGATGc         >  1:766893/1‑61 (MQ=255)
                                                                  |                                                          
TGCGCCCAGCAAAATACCGCGATGGCGGCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCG  >  minE/1866912‑1867036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: