Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,867,002	A→G	52.2%	I117I (ATT→ATC)	ygcS ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,867,002	0	A	G	52.2%	0.8 / 36.9	46	I117I (ATT→ATC)	ygcS	predicted transporter
Reads supporting (aligned to +/- strand):  ref base A (17/5);  new base G (15/9);  total (32/14)
Fisher's exact test for biased strand distribution p-value = 3.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

GCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCGTGGTGGCAAAAAATTGTAAAAACGAAGCAAGCGT  >  minE/1866939‑1867070                                                                |                                                                     gcgcGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCaa                                                                 <  1:2628499/69‑1 (MQ=255) gcgcGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCaa                                                                 <  1:918306/69‑1 (MQ=255) gcgcGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCaa                                                                 <  1:763999/69‑1 (MQ=255)                  ccagcAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATaa                                                <  1:2554001/69‑1 (MQ=255)                  ccagcAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATaa                                                <  1:2087004/69‑1 (MQ=255)                      cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                                                                 <  1:969913/48‑1 (MQ=255)                      cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                                                                 <  1:2401428/48‑1 (MQ=255)                      cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                                                                 <  1:3045009/48‑1 (MQ=255)                      cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                                                                 <  1:3045742/48‑1 (MQ=255)                      cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                                                                 <  1:1946538/48‑1 (MQ=255)                      cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                                                                 <  1:377890/48‑1 (MQ=255)                      cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                                                                 <  1:3333534/48‑1 (MQ=255)                      cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                                                                 <  1:127298/48‑1 (MQ=255)                      cAAGGTGTGACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCaa                                                                 <  1:1219052/48‑1 (MQ=255)                           tgtgACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATAAGATGc                                           >  1:2759461/1‑65 (MQ=255)                           tgtgACCTACTGAATAATCGCCTCCCAGAACAATGCCAATCAAAATGCGCAGTCCAATAAGATGc                                           >  1:355379/1‑65 (MQ=255)                              gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg                                    >  1:31245/1‑69 (MQ=255)                              gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg                                    >  1:32124/1‑69 (MQ=255)                              gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg                                    >  1:3040807/1‑69 (MQ=255)                              gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg                                    >  1:3025677/1‑69 (MQ=255)                              gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg                                    >  1:3303100/1‑69 (MQ=255)                              gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg                                    >  1:398331/1‑69 (MQ=255)                              gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg                                    >  1:2929913/1‑69 (MQ=255)                              gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg                                    >  1:624562/1‑69 (MQ=255)                              gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg                                    >  1:2685982/1‑69 (MQ=255)                              gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg                                    >  1:736913/1‑69 (MQ=255)                              gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg                                    >  1:2047434/1‑69 (MQ=255)                              gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg                                    >  1:1886477/1‑69 (MQ=255)                              gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg                                    >  1:1716159/1‑69 (MQ=255)                              gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg                                    >  1:1629384/1‑69 (MQ=255)                              gACCGACTGAGTAATCGCCTCCCAGACCAATGCCGATCAAAATGCGCAATCCAATAAGATGCTCTGGCg                                    >  1:1332504/1‑69 (MQ=255)                               aCCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCg                                    >  1:2920595/1‑68 (MQ=255)                               aCCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCg                                    >  1:753435/1‑68 (MQ=255)                               aCCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGACCAATAAGATGc                                           >  1:766893/1‑61 (MQ=255)                                                         cAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCGTGGTGGCAAAAAATTGTAAAAACGaa          >  1:75548/1‑68 (MQ=255)                                                         cAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCGTGGTGGCAAAAAATTGTAAAAACGaa          >  1:2942039/1‑68 (MQ=255)                                                         cAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCGTGGTGGCAAAAAATTGTAAAAACGaa          >  1:3329655/1‑68 (MQ=255)                                                         cAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCGTGGTGGCAAAAAATTGTAAAAACGaa          >  1:3232896/1‑68 (MQ=255)                                                         cAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCGTGGTGGCAAAAAATTGTAAAAACGaa          >  1:3062901/1‑68 (MQ=255)                                                         cAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCGTGGTGGCAAAAAATTGTAAAAACGaa          >  1:2611993/1‑68 (MQ=255)                                                         cAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCGTGGTGGCAAAAAATTGTAAAAACGaa          >  1:2273900/1‑68 (MQ=255)                                                         cAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCGTGGTGGCAAAAAATTGTAAAAACGaa          >  1:1325534/1‑68 (MQ=255)                                                         cAATGCCAAT‑AAAATGCGCAGTCCAATAAGATGCTCTGGCGTGGTGGCAAAAAATTGTAAAAACGaa          >  1:110874/1‑67 (MQ=255)                                                              ccAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCGTGGTGGCAAAAAATTGTAAAAACGaagcaag     >  1:363230/1‑68 (MQ=255)                                                              ccAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCGTGGTGGCAAAAAATTGTAAAAACGaagcaag     >  1:100723/1‑68 (MQ=255)                                                              ccAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCGTGGTGGCAAAAAATTGTAAAAACGaagcaag     >  1:2174914/1‑68 (MQ=255)                                                                aaTCAAAATGCGCAGTCCAATAAGATGCTCTGGCGTGGTGGCAAAAAATTGTAAAAACGAAGCAAGCGt  <  1:3223848/69‑1 (MQ=255)                                                                aaTCAAAATGCGCAGTCCAATAAGATGCTCTGGCGTGGTGGCAAAAAATTGTAAAAACGAAGCAAGCGt  <  1:287767/69‑1 (MQ=255)                                                                aaTCAAAATGCGCAGTCCAATAAGATGCTCTGGCGTGGTGGCAAAAAATTGTAAAAACGAAGCAAGCGt  <  1:2612953/69‑1 (MQ=255)                                                                aaTCAAAATGCGCAGTCCAATAAGATGCTCTGGCGTGGGGGCAAATAATTGTAAAAACGAAGCAAGCGt  <  1:1312487/69‑1 (MQ=255)                                                                |                                                                     GCGCGGGGAAAATTCAGCCAGCAAGGTGTGACCTACTGAATAATCGCCTCCCAGACCAATGCCAATCAAAATGCGCAGTCCAATAAGATGCTCTGGCGTGGTGGCAAAAAATTGTAAAAACGAAGCAAGCGT  >  minE/1866939‑1867070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑