| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,868,106 | G→A | 35.0% | D265D (GAC→GAT) | ygcU ← | predicted FAD containing dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,868,106 | 0 | G | A | 35.0% | 70.5 / 51.7 | 94 | D265D (GAC→GAT) | ygcU | predicted FAD containing dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (19/42); new base A (15/18); total (34/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAA > minE/1868047‑1868168 | aaGATCAGCACGCTTTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACaa > 1:1304997/1‑68 (MQ=255)aaGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACaa > 1:996027/1‑68 (MQ=255)aaGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACaa > 1:1648400/1‑68 (MQ=255)aaGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACaa > 1:2309456/1‑68 (MQ=255)aaGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACaa > 1:1041845/1‑68 (MQ=255) gATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACg < 1:2655580/69‑1 (MQ=255) gATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACg < 1:1602330/69‑1 (MQ=255) gATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACg < 1:2148352/69‑1 (MQ=255) aTCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACg < 1:781782/68‑1 (MQ=255) cAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGc < 1:1110001/69‑1 (MQ=255) aGCACGCATTTTCCGTCGGCTAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCg < 1:2341394/69‑1 (MQ=255) aGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCg < 1:1113053/69‑1 (MQ=255) aGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCg < 1:549324/69‑1 (MQ=255) aGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCg < 1:3430558/69‑1 (MQ=255) aGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCg < 1:1861492/69‑1 (MQ=255) aGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCg < 1:886414/69‑1 (MQ=255) aGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCg < 1:3135438/69‑1 (MQ=255) aGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCg < 1:1169390/69‑1 (MQ=255) aGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCg < 1:2598264/69‑1 (MQ=255) aGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGGCATACAAACGAGCg < 1:1068662/69‑1 (MQ=255) ttCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATTCAAACGAGCGATTgacggac > 1:3293625/1‑69 (MQ=255) ttCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTgacggac < 1:2030593/69‑1 (MQ=255) ttCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTgacggac > 1:3269741/1‑69 (MQ=255) ttCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTgacggac > 1:129776/1‑69 (MQ=255) ttCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTgacggac < 1:1791895/69‑1 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGcatcat > 1:1727114/1‑43 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATAc < 1:808494/69‑1 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACa > 1:1545746/1‑46 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGAt > 1:1667815/1‑67 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATa > 1:3400975/1‑68 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATa > 1:64794/1‑68 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATAc > 1:1724484/1‑69 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATAc > 1:1435777/1‑69 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATAc > 1:3334906/1‑69 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATAc > 1:288316/1‑69 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATAc > 1:2633777/1‑69 (MQ=255) aaTGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATACCCTTcc > 1:1756132/1‑68 (MQ=255) aaTGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATACCCTTcc > 1:2039051/1‑68 (MQ=255) aaTGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATACCCTTcc > 1:849025/1‑68 (MQ=255) aaTGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATACCCTTcc > 1:1755929/1‑68 (MQ=255) aaTGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATACCCTTcc > 1:2660618/1‑68 (MQ=255) aaTGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATACCCTTcc > 1:2181702/1‑68 (MQ=255) gggTGAAGTGTTGTGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:190496/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACg < 1:334349/40‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:1163617/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:2496363/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:1449635/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:940085/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:939438/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:1141345/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:2720634/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:2766762/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:3238198/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:1440234/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:1657497/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:1933360/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:1879192/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:1859393/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:594872/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:528403/56‑1 (MQ=255) ggTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCAt < 1:3156726/69‑1 (MQ=255) ggTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCAt < 1:3248891/69‑1 (MQ=255) ggTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCAt < 1:1041147/69‑1 (MQ=255) ggTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCAt < 1:258105/69‑1 (MQ=255) ggTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCAt < 1:1733276/69‑1 (MQ=255) gtgtTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGAttt < 1:1025267/68‑1 (MQ=255) gtgtTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGAttt < 1:2740489/68‑1 (MQ=255) gtgtTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGAttt < 1:99338/68‑1 (MQ=255) gtgtTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGAttt < 1:864324/68‑1 (MQ=255) gtgtTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGAttt < 1:1399360/68‑1 (MQ=255) gtgtTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGAttt < 1:1666437/68‑1 (MQ=255) gtgtTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGAttt < 1:197698/68‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:2134115/59‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:2972676/59‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:1268295/59‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:2076471/59‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:1552123/59‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:1724855/59‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:499281/59‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:1556149/59‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:532619/59‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:1569941/59‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:1612596/59‑1 (MQ=255) ccATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:2007406/39‑1 (MQ=25) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACGATGATTTCACGCAGGATGTTGa > 1:2097888/1‑68 (MQ=255) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGaa > 1:1340929/1‑69 (MQ=255) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGaa > 1:1627341/1‑69 (MQ=255) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGaa > 1:1337979/1‑69 (MQ=255) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGaa > 1:2938566/1‑69 (MQ=255) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGaa > 1:2387373/1‑69 (MQ=255) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGaa > 1:536647/1‑69 (MQ=255) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGaa > 1:3419303/1‑69 (MQ=255) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGaa > 1:1908528/1‑69 (MQ=255) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGaa > 1:1912391/1‑69 (MQ=255) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGaa > 1:3264592/1‑69 (MQ=255) | AAGATCAGCACGCATTTTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAA > minE/1868047‑1868168 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |