Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 139,702 G→T 17.8% A155A (GCC→GCA htrE ← predicted outer membrane usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE139,7020GT17.8% 272.2 / 36.3 128A155A (GCC→GCAhtrEpredicted outer membrane usher protein
Reads supporting (aligned to +/- strand):  ref base G (61/44);  new base T (10/13);  total (71/57)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

Only 100 of 132 total aligned reads displayed.

GCCGCATTAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAAT  >  minE/139637‑139770
                                                                 |                                                                    
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                             <  1:100206/69‑1 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                             >  1:1706373/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                             >  1:1905314/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                             >  1:2232853/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                             >  1:1624846/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                             >  1:268126/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                             >  1:2823959/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                             >  1:2907534/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                             >  1:1281586/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                             >  1:3298814/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                             >  1:1213071/1‑69 (MQ=255)
      ttAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGa                                                             >  1:1180834/1‑69 (MQ=255)
        aaTTCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                           >  1:2277998/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAg                                                                      >  1:1267584/1‑58 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTg                                                                 >  1:2939106/1‑63 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGaa                                                            >  1:3014777/1‑68 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                           >  1:126543/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                           >  1:90514/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                           >  1:1027762/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                           >  1:1627833/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                           >  1:2285136/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                           >  1:2376289/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                           >  1:2443457/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                           >  1:2611467/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                           >  1:1071774/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                           >  1:654654/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                           >  1:1364024/1‑69 (MQ=255)
        aaTGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAAc                                                           >  1:62521/1‑69 (MQ=255)
           gCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTCGAGGAACGTc                                                        <  1:1053444/69‑1 (MQ=255)
            ccGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTc                                                        >  1:933159/1‑68 (MQ=255)
                       tAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACg                                            <  1:2359837/69‑1 (MQ=255)
                       tAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACg                                            <  1:1648354/69‑1 (MQ=255)
                       tAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACg                                            <  1:1644478/69‑1 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCa                                                >  1:2778558/1‑63 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCa                                                >  1:1352630/1‑63 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                          >  1:1340056/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                          >  1:1529896/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                          >  1:2285845/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                          >  1:1603957/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                          <  1:2229606/69‑1 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                          >  1:2221525/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                          >  1:3416418/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                          >  1:1947245/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                          >  1:3239482/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                          >  1:2052282/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGtt                                          >  1:871359/1‑69 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGt                                           >  1:2103966/1‑68 (MQ=255)
                         aCGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGt                                           >  1:1158495/1‑68 (MQ=255)
                         aCGATGGATAAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGt                                           >  1:2943867/1‑68 (MQ=255)
                           gATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                         <  1:3380809/68‑1 (MQ=255)
                           gATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                         <  1:2649183/68‑1 (MQ=255)
                           gATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                         <  1:1512616/68‑1 (MQ=255)
                           gATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                         <  1:1818288/68‑1 (MQ=255)
                           gATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                         <  1:1932299/68‑1 (MQ=255)
                            aTGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                         <  1:1819848/67‑1 (MQ=255)
                              ggATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                         <  1:2964844/65‑1 (MQ=255)
                              ggATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                         <  1:60607/65‑1 (MQ=255)
                              ggATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                         <  1:3286438/65‑1 (MQ=255)
                              ggATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                         <  1:3035118/65‑1 (MQ=255)
                              ggATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                         <  1:78405/65‑1 (MQ=255)
                              ggATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                         <  1:2654917/65‑1 (MQ=255)
                              ggATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATc                                      >  1:138425/1‑68 (MQ=255)
                                 tCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACg                                            >  1:1881248/1‑59 (MQ=255)
                                 tCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACg                                            >  1:1737039/1‑59 (MQ=255)
                                 tCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGtt                                   <  1:78475/68‑1 (MQ=255)
                                 tCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGtt                                   <  1:811234/68‑1 (MQ=255)
                                 tCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGtt                                   <  1:2715569/68‑1 (MQ=255)
                                    aCATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTg                                         <  1:1129147/59‑1 (MQ=255)
                                      aTAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACg                               >  1:756191/1‑67 (MQ=255)
                                        aGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTATCGTCAt                           <  1:442784/69‑1 (MQ=255)
                                        aGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCAt                           <  1:467084/69‑1 (MQ=255)
                                        aGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCAt                           <  1:2103968/69‑1 (MQ=255)
                                         gTTTTGATAATTCTTCATAATCCATGCCTGTGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                          <  1:749256/69‑1 (MQ=255)
                                         gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                          <  1:3377166/69‑1 (MQ=255)
                                         gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                          <  1:2716367/69‑1 (MQ=255)
                                         gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                          <  1:1022105/69‑1 (MQ=255)
                                         gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                          <  1:3185350/69‑1 (MQ=255)
                                         gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                          <  1:205781/69‑1 (MQ=255)
                                         gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                          <  1:1607980/69‑1 (MQ=255)
                                         gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                          <  1:1444888/69‑1 (MQ=255)
                                         gTTTTGATAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                          <  1:1542301/69‑1 (MQ=255)
                                         gTTTTGATAATTCATCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTa                          <  1:2322446/69‑1 (MQ=255)
                                               atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                     >  1:3187997/2‑68 (MQ=255)
                                               atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                     >  1:2063249/2‑68 (MQ=255)
                                               atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                     >  1:2141195/2‑68 (MQ=255)
                                               atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                     >  1:2365083/2‑68 (MQ=255)
                                               atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                     >  1:558154/2‑68 (MQ=255)
                                               atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                     >  1:2474370/2‑68 (MQ=255)
                                               atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                     >  1:2896663/2‑68 (MQ=255)
                                               atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                     >  1:3069268/2‑68 (MQ=255)
                                               atAATTCTTCATAATCCATGCCTGGGGGACATCGATATCCAGGCGTTGATCGTTAACATCGTAACGaa                     >  1:3082853/2‑68 (MQ=255)
                                                    ttttCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGaa                <  1:2434839/68‑1 (MQ=255)
                                                            aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg       <  1:1713788/69‑1 (MQ=255)
                                                            aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg       <  1:717558/69‑1 (MQ=255)
                                                            aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg       <  1:1256283/69‑1 (MQ=255)
                                                            aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg       <  1:2028433/69‑1 (MQ=255)
                                                            aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg       <  1:1861666/69‑1 (MQ=255)
                                                            aCCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAggg       <  1:1014071/69‑1 (MQ=255)
                                                                 ggCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAAt  >  1:1346114/1‑69 (MQ=255)
                                                                 ggCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAAt  >  1:1825668/1‑69 (MQ=255)
                                                                 |                                                                    
GCCGCATTAATGCCGTTTTCCCATAACGATGGATCAACATAGTTTTGGTAATTTTTCATTACCCAGGCTTGAGGAACGTCTATATCCAGACGTTGATCGTTAACGTCATAACGAACAGAAGCCTGAGGGATAAT  >  minE/139637‑139770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: