| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,868,124 | T→C | 31.9% | S259S (TCA→TCG) | ygcU ← | predicted FAD containing dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,868,124 | 0 | T | C | 31.9% | 83.8 / 51.3 | 100 | S259S (TCA→TCG) | ygcU | predicted FAD containing dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (21/47); new base C (14/18); total (35/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 106 total aligned reads displayed.
TTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGTTTTCATGTCTTCCAGGA > minE/1868063‑1868191 | ttCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATTCAAACGAGCGATTgacggac > 1:3293625/1‑69 (MQ=255)ttCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTgacggac < 1:2030593/69‑1 (MQ=255)ttCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTgacggac < 1:1791895/69‑1 (MQ=255)ttCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTgacggac > 1:3269741/1‑69 (MQ=255)ttCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTgacggac > 1:129776/1‑69 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATAc < 1:808494/69‑1 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGAt > 1:1667815/1‑67 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATa > 1:64794/1‑68 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATa > 1:3400975/1‑68 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATAc > 1:1724484/1‑69 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATAc > 1:2633777/1‑69 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATAc > 1:3334906/1‑69 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATAc > 1:1435777/1‑69 (MQ=255) tCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATAc > 1:288316/1‑69 (MQ=255) aaTGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATACCCTTcc > 1:849025/1‑68 (MQ=255) aaTGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATACCCTTcc > 1:2181702/1‑68 (MQ=255) aaTGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATACCCTTcc > 1:2660618/1‑68 (MQ=255) aaTGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATACCCTTcc > 1:2039051/1‑68 (MQ=255) aaTGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATACCCTTcc > 1:1755929/1‑68 (MQ=255) aaTGGGTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGATACCCTTcc > 1:1756132/1‑68 (MQ=255) gggTGAAGTGTTGTGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:190496/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:940085/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:1859393/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:1879192/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:1933360/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:2496363/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:2720634/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:2766762/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:1657497/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:3238198/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:1449635/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:1440234/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:528403/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:594872/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:1163617/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:1141345/56‑1 (MQ=255) gggTGAAGTGTTGGGTGCCATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:939438/56‑1 (MQ=255) ggTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCAt < 1:3156726/69‑1 (MQ=255) ggTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCAt < 1:3248891/69‑1 (MQ=255) ggTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCAt < 1:258105/69‑1 (MQ=255) ggTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCAt < 1:1041147/69‑1 (MQ=255) ggTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCAt < 1:1733276/69‑1 (MQ=255) gtgtTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGAttt < 1:1025267/68‑1 (MQ=255) gtgtTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGAttt < 1:864324/68‑1 (MQ=255) gtgtTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGAttt < 1:1399360/68‑1 (MQ=255) gtgtTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGAttt < 1:1666437/68‑1 (MQ=255) gtgtTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGAttt < 1:197698/68‑1 (MQ=255) gtgtTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGAttt < 1:2740489/68‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:1612596/59‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:1268295/59‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:532619/59‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:499281/59‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:1556149/59‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:1569941/59‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:2972676/59‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:1724855/59‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:2134115/59‑1 (MQ=255) gggTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:2076471/59‑1 (MQ=255) ccATCTTCAGCATCATACAAACGAGCAATCGACGGACGa < 1:2007406/39‑1 (MQ=25) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACGATGATTTCACGCAGGATGTTGa > 1:2097888/1‑68 (MQ=255) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGaa > 1:3264592/1‑69 (MQ=255) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGaa > 1:1912391/1‑69 (MQ=255) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGaa > 1:3419303/1‑69 (MQ=255) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGaa > 1:1340929/1‑69 (MQ=255) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGaa > 1:536647/1‑69 (MQ=255) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGaa > 1:1337979/1‑69 (MQ=255) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGaa > 1:1627341/1‑69 (MQ=255) ttCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGaa > 1:2938566/1‑69 (MQ=255) tCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGtt > 1:1071885/1‑69 (MQ=255) aTACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGTATGTTGAAGCCGGtttt < 1:1409217/69‑1 (MQ=255) aTACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGtttt < 1:3171389/69‑1 (MQ=255) aTACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGtttt < 1:903286/69‑1 (MQ=255) aTACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGtttt < 1:2508707/69‑1 (MQ=255) aTACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGtttt > 1:3267699/1‑69 (MQ=255) aTACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGtttt < 1:2873935/69‑1 (MQ=255) aTACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGtttt < 1:1422480/69‑1 (MQ=255) aTACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGtttt < 1:3335708/69‑1 (MQ=255) aTACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGtttt < 1:337983/69‑1 (MQ=255) aTACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGtttt < 1:917492/69‑1 (MQ=255) aTACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGtttt < 1:923436/69‑1 (MQ=255) aTACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGtttt < 1:2785187/69‑1 (MQ=255) aTACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGtttt < 1:376705/69‑1 (MQ=255) aTACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGtttt < 1:435041/69‑1 (MQ=255) aTACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGtttt < 1:2740488/69‑1 (MQ=255) tACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGtttt < 1:1938993/68‑1 (MQ=255) aCAAACGAGCGATTGACGGACGATACCCTTCCACCATg < 1:1992067/38‑1 (MQ=255) cAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGTTTTCa > 1:2378655/1‑68 (MQ=255) cAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGTTTTCa > 1:207877/1‑68 (MQ=255) cAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGTTTTCAt > 1:1134834/1‑69 (MQ=255) cAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGTTTTCAt > 1:2832647/1‑69 (MQ=255) cAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGTTTTCAt > 1:2799459/1‑69 (MQ=255) cGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGTTTTCATGTCt < 1:1082294/69‑1 (MQ=255) aGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGtttt < 1:2067117/60‑1 (MQ=255) aTTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGTTTTCATGTCTTCCAgg < 1:1666549/69‑1 (MQ=255) ttGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGTTTTCATGTCTTCCAGGa < 1:456159/69‑1 (MQ=255) ttGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGTTTTCATGTCTTCCAGGa < 1:3405740/69‑1 (MQ=255) ttGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGTTTTCATGTCTTCCAGGa < 1:2175394/69‑1 (MQ=255) ttGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGTTTTCATGTCTTCCAGGa < 1:2435504/69‑1 (MQ=255) ttGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGTTTTCATGTCTTCCAGGa < 1:246446/69‑1 (MQ=255) ttGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGTTTTCATGTCTTCCAGGa < 1:912893/69‑1 (MQ=255) | TTCCGTCGGCAAAATGGGTGAAGTGTTGGGTGCCATCTTCAGCGTCATACAAACGAGCGATTGACGGACGATACCCTTCCACCATGATTTCACGCAGGATGTTGAAGCCGGTTTTCATGTCTTCCAGGA > minE/1868063‑1868191 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |