| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,869,696 | G→T | 33.8% | T20T (ACC→ACA) | ygcW ← | predicted deoxygluconate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,869,696 | 0 | G | T | 33.8% | 159.4 / 99.6 | 142 | T20T (ACC→ACA) | ygcW | predicted deoxygluconate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (29/65); new base T (26/22); total (55/87) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.04e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01 | |||||||||||
Only 100 of 142 total aligned reads displayed.
AGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTT > minE/1869633‑1869759 | aGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGttt < 1:276526/68‑1 (MQ=255) cTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAc < 1:8236/69‑1 (MQ=255) cTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAc < 1:174440/69‑1 (MQ=255) cTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAc < 1:2258661/69‑1 (MQ=255) cTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAc < 1:2410810/69‑1 (MQ=255) cTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAc < 1:2517570/69‑1 (MQ=255) cTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAc < 1:2729739/69‑1 (MQ=255) cTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAc < 1:2881763/69‑1 (MQ=255) cTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAc < 1:3108313/69‑1 (MQ=255) cTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAc < 1:3178940/69‑1 (MQ=255) cTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAc < 1:1195339/69‑1 (MQ=255) cTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAc < 1:3414354/69‑1 (MQ=255) cTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTAc < 1:354276/69‑1 (MQ=255) tttGGCCAACGCATTGGCAAATGCCTGGCGTAAACCGATATTCCCACCGGTAAGAATTGCGGTTTTag < 1:2512514/68‑2 (MQ=255) cAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGTTTTACCTTTCa > 1:3172870/1‑68 (MQ=255) cAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGTTTTACCTTTCa > 1:3251363/1‑68 (MQ=255) cAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGTTTTACCTTTCa > 1:774826/1‑68 (MQ=255) cAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGTTTTACCTTTCa > 1:968747/1‑68 (MQ=255) cAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGTTTTACCTTTCa > 1:1718508/1‑68 (MQ=255) cAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGTTTTACCTTTCa > 1:1013997/1‑68 (MQ=255) cAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGTTTTACCTTTCa > 1:1285854/1‑68 (MQ=255) gCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGaaaaaa < 1:2546252/69‑1 (MQ=255) aaaTGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATc < 1:2681938/69‑1 (MQ=255) aaaTGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATc < 1:1095533/69‑1 (MQ=255) tGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGttt < 1:3062160/44‑1 (MQ=255) tGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGttt < 1:3280522/44‑1 (MQ=255) tGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGttt < 1:3380067/44‑1 (MQ=255) tGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGttt < 1:1153671/44‑1 (MQ=255) tGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGttt < 1:1716114/44‑1 (MQ=255) tGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGttt < 1:636841/44‑1 (MQ=255) tGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGttt < 1:811719/44‑1 (MQ=255) tGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCa > 1:2525530/1‑68 (MQ=255) tGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCa > 1:281005/1‑68 (MQ=255) tGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCa > 1:2198818/1‑68 (MQ=255) tGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTgag < 1:595607/69‑1 (MQ=255) gCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGttt < 1:2955121/38‑1 (MQ=38) gCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGttt < 1:141027/38‑1 (MQ=38) gCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGttt < 1:269612/38‑1 (MQ=38) gCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGttt < 1:2474533/38‑1 (MQ=38) gCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGttt < 1:2439262/38‑1 (MQ=38) gCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGttt < 1:1465006/38‑1 (MQ=38) gCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGttt < 1:2785283/38‑1 (MQ=38) gCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGttt < 1:2191763/38‑1 (MQ=38) gCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGttt < 1:2957521/38‑1 (MQ=38) gCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGttt < 1:300934/38‑1 (MQ=38) gCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGttt < 1:770039/38‑1 (MQ=38) gCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGttt < 1:2024994/38‑1 (MQ=38) gCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGttt < 1:1952804/38‑1 (MQ=38) gCCTAAACCGCTATTCCCGCCGGTAACAATTGCTGttt < 1:696060/38‑1 (MQ=38) cTAAACCGCTATTCCCACCTGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACg < 1:180449/69‑1 (MQ=255) cTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACg < 1:2809803/69‑1 (MQ=255) cTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACg < 1:1348384/69‑1 (MQ=255) cTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACg < 1:847274/69‑1 (MQ=255) cTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACg < 1:545412/69‑1 (MQ=255) cTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACg < 1:198034/69‑1 (MQ=255) tAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACg < 1:1796456/68‑1 (MQ=255) ccGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCAtt < 1:1593560/68‑1 (MQ=255) ccGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCAtt < 1:159672/68‑1 (MQ=255) ccGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCAtt < 1:2428406/68‑1 (MQ=255) ccGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCAtt < 1:2402209/68‑1 (MQ=255) ccGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCAtt < 1:1951722/68‑1 (MQ=255) ccGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCAtt < 1:1862522/68‑1 (MQ=255) ccGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCAtt < 1:604266/68‑1 (MQ=255) cTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTgaga < 1:2592337/69‑1 (MQ=255) cTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTgaga < 1:1251514/69‑1 (MQ=255) cTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTgaga < 1:2547645/69‑1 (MQ=255) cTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTgaga < 1:2326139/69‑1 (MQ=255) cTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTgaga < 1:172693/69‑1 (MQ=255) aTTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTgagag > 1:3103579/1‑68 (MQ=255) cccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTgaga > 1:1563391/1‑64 (MQ=255) cccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGAt < 1:1834008/67‑1 (MQ=255) cccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGAt < 1:471880/67‑1 (MQ=255) cccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTc > 1:579275/1‑69 (MQ=255) cccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTc > 1:1918813/1‑69 (MQ=255) cccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTc > 1:1208187/1‑69 (MQ=255) cccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTc > 1:127321/1‑69 (MQ=255) cccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTc > 1:2604058/1‑69 (MQ=255) cccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTc > 1:1507272/1‑69 (MQ=255) cccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTc > 1:3149574/1‑69 (MQ=255) ccACCGGTAACAATTGCGGTTTTACCTTTCAGTGAGAAAAAATCCATTGAGAACGCATTGAGAGAt < 1:2041014/66‑1 (MQ=255) cgccGGTAACAATTGCTGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGc > 1:3076169/3‑55 (MQ=255) cgccGGTAACAATTGCTGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCa > 1:2933150/3‑56 (MQ=255) cgccGGTAACAATTGCTGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCa > 1:1002073/3‑56 (MQ=255) cgccGGTAACAATTGCTGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCa > 1:2500512/3‑56 (MQ=255) cgccGGTAACAATTGCTGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCa > 1:1024519/3‑56 (MQ=255) cgccGGTAACAATTGCTGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCa > 1:2773127/3‑56 (MQ=255) cgccGGTAACAATTGCTGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCa > 1:717133/3‑56 (MQ=255) cgccGGTAACAATTGCTGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCa > 1:1822279/3‑56 (MQ=255) cgccGGTAACAATTGCTGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCa > 1:1278477/3‑56 (MQ=255) cgccGGTAACAATTGCTGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCa > 1:214435/3‑56 (MQ=255) cgccGGTAACAATTGCTGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCa > 1:406339/3‑56 (MQ=255) cgccGGTAACAATTGCTGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCa > 1:1934757/3‑56 (MQ=255) cgccGGTAACAATTGCTGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCa > 1:2070992/3‑56 (MQ=255) aTTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAg > 1:209514/1‑67 (MQ=255) aTTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGtt > 1:622606/1‑69 (MQ=255) aTTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGtt > 1:227696/1‑69 (MQ=255) aTTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGtt > 1:1431723/1‑69 (MQ=255) aTTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGtt > 1:3263821/1‑69 (MQ=255) aTTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGtt > 1:956367/1‑69 (MQ=255) aTTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGt > 1:1218968/1‑68 (MQ=255) | AGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTT > minE/1869633‑1869759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |