| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,874,131 | G→A | 30.7% | intergenic (+1275/+98) | ygcE → / ← ygcF | predicted kinase/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,874,131 | 0 | G | A | 30.7% | 75.8 / 37.2 | 81 | intergenic (+1275/+98) | ygcE/ygcF | predicted kinase/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (22/34); new base A (16/9); total (38/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.42e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATTTTCACTCCAGCGAAAAATTAATGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGAAGTCTTTACAGG > minE/1874067‑1874195 | aatTTTCACTCCAGCGAAAAATTAATGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATg > 1:2308117/1‑68 (MQ=255)aatTTTCACTCCAGCGAAAAATTAATGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATg > 1:3205161/1‑68 (MQ=255)aatTTTCACTCCAGCGAAAAATTAATGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATg > 1:3077076/1‑68 (MQ=255)aatTTTCACTCCAGCGAAAAATTAATGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATg > 1:2841105/1‑68 (MQ=255)aatTTTCACTCCAGCGAAAAATTAATGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATg > 1:2533588/1‑68 (MQ=255)aatTTTCACTCCAGCGAAAAATTAATGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATg > 1:2503430/1‑68 (MQ=255)aatTTTCACTCCAGCGAAAAATTAATGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATg > 1:1078955/1‑68 (MQ=255) ttCACTCCAGCGAAAAATTAATGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATGGGTaa < 1:2215007/69‑1 (MQ=255) ttCACTCCAGCGAAAAATTAATGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATGGGTaa < 1:2865661/69‑1 (MQ=255) cACTCCAGCGAAAAATTAACGCCACAGAATTTGTAGAGAATGATAATGATTAGTCCAGAATg > 1:101513/1‑62 (MQ=255) cACTCCAGCGAAAAATTAACGCCACAGAATTTGTAGAGAATGATAATGATTAGTCCAGAATg > 1:2119796/1‑62 (MQ=255) cACTCCAGCGAAAAATTAACGCCACAGAATTTGTAGAGAATGATAATGATTAGTCCAGAATg > 1:779209/1‑62 (MQ=255) cACTCCAGCGAAAAATTAACGCCACAGAATTTGTAGAGAATGATAATGATTAGTCCAGAATg > 1:2032227/1‑62 (MQ=255) cACTCCAGCGAAAAATTAACGCCACAGAATTTGTAGAGAATGATAATGATTAGTCCAGAATg > 1:2292082/1‑62 (MQ=255) cACTCCAGCGAAAAATTAACGCCACAGAATTTGTAGAGAATGATAATGATTAGTCCAGAATg > 1:930569/1‑62 (MQ=255) cACTCCAGCGAAAAATTAACGCCACAGAATTTGTAGAGAATGATAATGATTAGTCCAGAATg > 1:1877395/1‑62 (MQ=255) cACTCCAGCGAAAAATTAACGCCACAGAATTTGTAGAGAATGATAATGATTAGTCCAGAATg > 1:3006424/1‑62 (MQ=255) cACTCCAGCGAAAAATTAACGCCACAGAATTTGTAGAGAATGATAATGATTAGTCCAGAATg > 1:1628430/1‑62 (MQ=255) cACTCCAGCGAAAAATTAACGCCACAGAATTTGTAGAGAATGATAATGATTAGTCCAGAATg > 1:400698/1‑62 (MQ=255) cACTCCAGCGAAAAATTAACGCCACAGAATTTGTAGAGAATGATAATGATTAGTCCAGAATg > 1:3422958/1‑62 (MQ=255) cACTCCAGCGAAAAATTAACGCCACAGAATTTGTAGAGAATGATAATGATTAGTCCAGAATg > 1:1019957/1‑62 (MQ=255) cACTCCAGCGAAAAATTAACGCCACAGAATTTGTAGAGAATGATAATGATTAGTCCAGAATg > 1:947608/1‑62 (MQ=255) cACTCCAGCGAAAAATTAACGCCACAGAATTTGTAGAGAATGATAATGATTAGTCCAGAATg > 1:3141654/1‑62 (MQ=255) cACTCCAGCGAAAAATTAACGCCACAGAATTTGTAGAGAATGATAATGATTAGTCCAGAATg > 1:2723972/1‑62 (MQ=255) cACTCCAGCGAAAAATTAACGCCACAGAATTTGT‑‑AGAATGATAATGATTAGTCCAGAATg > 1:955096/1‑60 (MQ=255) cGAAAAATTAATGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATGGGTAAAATGgaggag < 1:453962/69‑1 (MQ=255) aaaTTAATGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGtttt < 1:1117312/69‑1 (MQ=255) aaaTTAATGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGtttt < 1:2480045/69‑1 (MQ=255) aaaTTAATGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGtttt < 1:2667964/69‑1 (MQ=255) aaaTTAATGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGtttt < 1:1993634/69‑1 (MQ=255) aaTTAATGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGtttt < 1:3095853/68‑1 (MQ=255) tAATGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCaga < 1:650210/69‑1 (MQ=255) tAATGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCaga < 1:285367/69‑1 (MQ=255) aaTGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCaga < 1:3371628/68‑1 (MQ=255) acaGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATGGGTAAAATGGAGGACTTTTCAGAGGCGaca < 1:3206970/69‑1 (MQ=255) tGTAGAGAATGCTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGtttt < 1:2744961/50‑1 (MQ=255) tAGAGAATGCTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAAtata < 1:2074045/68‑1 (MQ=255) tAGAGAATGCTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAAtata < 1:1356914/68‑1 (MQ=255) tAGAGAATGCTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAAtata < 1:1495003/68‑1 (MQ=255) tAGAGAATGCTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAAtata < 1:1695758/68‑1 (MQ=255) tAGAGAATGCTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAAtata < 1:1703746/68‑1 (MQ=255) tAGAGAATGCTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAAtata < 1:2117153/68‑1 (MQ=255) cTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGa > 1:2881611/1‑69 (MQ=255) cTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGa > 1:292865/1‑69 (MQ=255) cTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGa > 1:3120020/1‑69 (MQ=255) cTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGa > 1:2928788/1‑69 (MQ=255) cTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGa > 1:2328991/1‑69 (MQ=255) cTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGa > 1:1076224/1‑69 (MQ=255) cTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGa > 1:1698340/1‑69 (MQ=255) cTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGa > 1:1764491/1‑69 (MQ=255) aTGATTAGTCCAGAATGGGCAAAATGGAGG‑TTTTTCAGAGACGACAAAGAATATAAATGAGTAGAAGTc < 1:1061500/69‑1 (MQ=255) aTGATTAGTCCAGAATGGGCAAAATGGAGG‑TTTTTCAGAGACGACAAAGAATATAAATGAGTAGAAGTc < 1:141238/69‑1 (MQ=255) aTGATTAGTCCAGAATGGGCAAAATGGAGG‑TTTTTCAGAGACGACAAAGAATATAAATGAGTAGAAGTc < 1:1755942/69‑1 (MQ=255) aTGATTAGTCCAGAATGGGCAAAATGGAGG‑TTTTTCAGAGACGACAAAGAATATAAATGAGTAGAAGTc < 1:835502/69‑1 (MQ=255) aTGATTAGTCCAGAATGGGCAAAATGGAGG‑TTTTTCAGAGACGACAAAGAATATAAATGAGTAGAAGTc < 1:3383392/69‑1 (MQ=255) aTGATTAGTCCAGAATGGGCAAAATGGAGG‑TTTTTCAGAGACGACAAAGAATATAAATGAGTAGAAGTc < 1:2318221/69‑1 (MQ=255) aTGATTAGTCCAGAATGGGCAAAATGGAGG‑TTTTTCAGAGACGACAAAGAATATAAATGAGTAGAAGTc < 1:548170/69‑1 (MQ=255) aTGATTAGTCCAGAATGGGCAAAATGGAGG‑TTTTTCAGAGACGACAAAGAATATAAATGAGTAGAAGTc < 1:2479705/69‑1 (MQ=255) aTGATTAGTCCAGAATGGGCAAAATGGAGG‑TTTTTCAGAGACGACAAAGAATATAAATGAGTAGAAGTc < 1:2688319/69‑1 (MQ=255) gATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGAAGt < 1:1259453/67‑1 (MQ=255) gATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGAAGTc > 1:3050341/1‑68 (MQ=255) gATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGAAGTc > 1:1454054/1‑68 (MQ=255) gATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGAAGTc > 1:99323/1‑68 (MQ=255) aTTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGacaaac < 1:1004940/47‑1 (MQ=255) aTTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGacaaac < 1:1307781/47‑1 (MQ=255) aTTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGacaaac < 1:2940740/47‑1 (MQ=255) aTTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGacaaac < 1:139793/47‑1 (MQ=255) aTTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGacaaac < 1:2030069/47‑1 (MQ=255) aTTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGacaaac < 1:2384738/47‑1 (MQ=255) aTTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGacaaac < 1:2177358/47‑1 (MQ=255) aTTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGAAGTCtt > 1:2986228/1‑69 (MQ=255) aTTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGAAGTCtt > 1:896393/1‑69 (MQ=255) aTTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGAAGTCtt > 1:2323733/1‑69 (MQ=255) aTTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGAAGTCt > 1:2335982/1‑68 (MQ=255) tAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGAAGTCtt < 1:1000752/67‑1 (MQ=255) cccTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGAAGTCTTTACa < 1:796471/68‑1 (MQ=255) cccTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGAAGTCTTTACa < 1:2502946/68‑1 (MQ=255) ccTGGATGTGTAAAATGGAGTAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGAAGTCTTTACa < 1:3009893/67‑1 (MQ=255) ccTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGAAGTCTTTACa < 1:391953/67‑1 (MQ=255) ccTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGAAGTCTTTACAgg < 1:202501/69‑1 (MQ=255) ccTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGAAGTCTTTACAgg < 1:2528843/69‑1 (MQ=255) | AATTTTCACTCCAGCGAAAAATTAATGCCACAGAATTTGTAGAGAATGCTAATGATTAGCCCTGGATGGGTAAAATGGAGGAGTTTTCAGAGGCGACAAACAATATAAATGAGTAGAAGTCTTTACAGG > minE/1874067‑1874195 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |