Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,876,356 G→A 31.5% I356I (ATC→ATT)  eno ← enolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,876,3560GA31.5% 106.8 / 56.5 114I356I (ATC→ATT) enoenolase
Reads supporting (aligned to +/- strand):  ref base G (29/49);  new base A (12/24);  total (41/73)
Fisher's exact test for biased strand distribution p-value = 8.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 114 total aligned reads displayed.

TTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATA  >  minE/1876302‑1876422
                                                      |                                                                  
ttCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGt                                                      >  1:1445991/1‑69 (MQ=255)
               agagaTAACTGCAGTTTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:2400707/60‑1 (MQ=255)
               agagaTAACTGCAGTGTAGCCAGCGTCTTTGGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:905711/60‑1 (MQ=255)
               agagaTAACTGCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:2273455/60‑1 (MQ=255)
               agagaTAACTGCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:212237/60‑1 (MQ=255)
               agagaTAACTGCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:1878094/60‑1 (MQ=255)
               agagaTAACTGCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:1876623/60‑1 (MQ=255)
               agagaTAACTGCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:2583812/60‑1 (MQ=255)
               agagaTAACTGCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:1801120/60‑1 (MQ=255)
               agagaTAACTGCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:2395280/60‑1 (MQ=255)
               agagaTAACTGCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:2740610/60‑1 (MQ=255)
               agagaTAACTGCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:2893072/60‑1 (MQ=255)
               agagaTAACTGCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:2905728/60‑1 (MQ=255)
               agagaTAACTGCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:2301444/60‑1 (MQ=255)
               agagaTAACTGCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:3144923/60‑1 (MQ=255)
               agagaTAACTGCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:372551/60‑1 (MQ=255)
               agagaTAACTGCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:1344561/60‑1 (MQ=255)
               agagaTAACTGCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:1331990/60‑1 (MQ=255)
               agagaTAACTGCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:1257693/60‑1 (MQ=255)
               agagaTAACTGCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:766802/60‑1 (MQ=255)
               agagaTAACTGCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGCTTCGGt                                                <  1:438216/60‑1 (MQ=255)
               agagaTAACTCCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:2499520/60‑1 (MQ=255)
                gagaTAACTGCAGTGTAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGt                                                <  1:590673/59‑1 (MQ=255)
                 agaTAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCg                                      <  1:2049076/68‑1 (MQ=255)
                 agaTAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCg                                      <  1:597209/68‑1 (MQ=255)
                 agaTAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCg                                      <  1:804237/68‑1 (MQ=255)
                 agaTAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCg                                      <  1:1904490/68‑1 (MQ=255)
                     aaCTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCg                                      >  1:538399/1‑64 (MQ=255)
                     aaCTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTg                                 >  1:3166068/1‑69 (MQ=255)
                     aaCTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTg                                 >  1:848196/1‑69 (MQ=255)
                     aaCTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTg                                 >  1:1183545/1‑69 (MQ=255)
                             tgtAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGTCAGAGAACCGATCTGGTTGAAt                          >  1:3007449/1‑68 (MQ=255)
                             tgtAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGTCAGAGAACCGATCTGGTTGAAt                          >  1:2405456/1‑68 (MQ=255)
                             tgtAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGTCAGAGAACCGATCTGGTTGAAt                          >  1:2703956/1‑68 (MQ=255)
                             tgtAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGTCAGAGAACCGATCTGGTTGAAt                          >  1:2670739/1‑68 (MQ=255)
                             tgtAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGTCAGAGAACCGATCTGGTTGAAt                          >  1:928691/1‑68 (MQ=255)
                             tgtAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGTCAGAGAACCGATCTGGTTGAAt                          >  1:1007080/1‑68 (MQ=255)
                             tgtAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGTCAGAGAACCGATCTGGTTGAAt                          >  1:2154485/1‑68 (MQ=255)
                             tgtAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGTCAGAGAACCGATCTGGTTGAAt                          >  1:2133811/1‑68 (MQ=255)
                             tgtAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGTCAGAGAACCGATCTGGTTGAAt                          >  1:1095071/1‑68 (MQ=255)
                             tgtAGCCAGCGTCTTTCGCCATCTTAATAGCAGCCAGGGTTTCGGTCAGAGAACCGATCTGGTTGAAt                          >  1:101648/1‑68 (MQ=255)
                              gtAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAAttt                        <  1:627555/69‑1 (MQ=255)
                              gtAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAAttt                        <  1:296716/69‑1 (MQ=255)
                               tAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTg                       >  1:3335871/1‑69 (MQ=255)
                               tAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTg                       >  1:2853494/1‑69 (MQ=255)
                               tAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTg                       <  1:1001729/69‑1 (MQ=255)
                                    aGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAg                  <  1:23038/69‑1 (MQ=255)
                                    aGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAg                  <  1:2011525/69‑1 (MQ=255)
                                    aGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAg                  <  1:883792/69‑1 (MQ=255)
                                    aGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAg                  <  1:868922/69‑1 (MQ=255)
                                     gCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAg                  <  1:428262/68‑1 (MQ=255)
                                       aTCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAgaga                                          >  1:2570030/1‑42 (MQ=255)
                                       aTCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAgaga                                          >  1:3068952/1‑42 (MQ=255)
                                       aTCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAgaga                                          >  1:3149122/1‑42 (MQ=255)
                                       aTCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGa                >  1:2952894/1‑68 (MQ=255)
                                       aTCTTTCGACATCTTGATTGCAGCCAGAGTTTCGGTCAgaga                                          >  1:1810599/1‑42 (MQ=255)
                                          tttCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATgg             <  1:1691163/68‑1 (MQ=255)
                                          tttCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATgg             <  1:788746/68‑1 (MQ=255)
                                          tttCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATgg             <  1:3252607/68‑1 (MQ=255)
                                          tttCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGa            >  1:1623908/1‑69 (MQ=255)
                                              gCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGtt         <  1:589984/68‑1 (MQ=255)
                                              gCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGtt         <  1:2255828/68‑1 (MQ=255)
                                              gCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGtt         <  1:2532836/68‑1 (MQ=255)
                                                cATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGc      <  1:3083836/69‑1 (MQ=255)
                                                cATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGc      <  1:1387316/69‑1 (MQ=255)
                                                cATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGc      <  1:527949/69‑1 (MQ=255)
                                                cATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGc      <  1:3400363/69‑1 (MQ=255)
                                                cATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGc      <  1:613107/69‑1 (MQ=255)
                                                cATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGc      <  1:883622/69‑1 (MQ=255)
                                                cATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGc      <  1:2874266/69‑1 (MQ=255)
                                                cATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGc      <  1:2581464/69‑1 (MQ=255)
                                                  tCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGc      <  1:3079907/67‑1 (MQ=255)
                                                   cTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGAt   <  1:789202/69‑1 (MQ=255)
                                                   cTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGAt   <  1:1009434/69‑1 (MQ=255)
                                                   cTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGAt   <  1:1192875/69‑1 (MQ=255)
                                                   cTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGAt   <  1:79773/69‑1 (MQ=255)
                                                   cTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGAt   <  1:32477/69‑1 (MQ=255)
                                                   cTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGAt   <  1:419921/69‑1 (MQ=255)
                                                   cTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGAt   <  1:1406289/69‑1 (MQ=255)
                                                   cTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGAt   <  1:3304738/69‑1 (MQ=255)
                                                   cTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGAt   <  1:1483505/69‑1 (MQ=255)
                                                   cTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGAt   <  1:2569472/69‑1 (MQ=255)
                                                   cTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGAt   <  1:1665121/69‑1 (MQ=255)
                                                   cTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGAt   <  1:2778501/69‑1 (MQ=255)
                                                   cTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCAAt   <  1:421538/69‑1 (MQ=255)
                                                    ttGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAg                  >  1:740786/1‑53 (MQ=255)
                                                    ttGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAg                  >  1:1069396/1‑53 (MQ=255)
                                                    ttGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGc      >  1:915965/1‑65 (MQ=255)
                                                    ttGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGc      >  1:1571478/1‑65 (MQ=255)
                                                    ttGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGa    >  1:2162921/1‑67 (MQ=255)
                                                    ttGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGAt   <  1:2183059/68‑1 (MQ=255)
                                                    ttGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGAt   <  1:1781604/68‑1 (MQ=255)
                                                    ttGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATa  >  1:781148/1‑69 (MQ=255)
                                                    ttGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATa  >  1:2595503/1‑69 (MQ=255)
                                                    ttGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATa  >  1:3046917/1‑69 (MQ=255)
                                                    ttGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATa  >  1:1847757/1‑69 (MQ=255)
                                                    ttGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATa  >  1:1133409/1‑69 (MQ=255)
                                                    ttGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATa  >  1:1133747/1‑69 (MQ=255)
                                                    ttGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATa  >  1:35409/1‑69 (MQ=255)
                                                    ttGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTAGATCAGGATGGAGTTAGCGATa  >  1:159495/1‑69 (MQ=255)
                                                      |                                                                  
TTCGCCAGAACGGTGAGAGATAACTGCAGTGTAGCCAGCATCTTTCGCCATCTTGATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATA  >  minE/1876302‑1876422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑